PMID- 30628827
OWN - NLM
STAT- MEDLINE
DCOM- 20191125
LR  - 20191125
IS  - 1802-9973 (Electronic)
IS  - 0862-8408 (Linking)
VI  - 68
IP  - 2
DP  - 2019 Apr 30
TI  - The H19/let-7 feedback loop contributes to developmental dysplasia and 
      dislocation of the hip.
PG  - 275-284
AB  - Developmental dysplasia and dislocation of the hip (DDH) is the most common type 
      of lower limb deformity in pediatric orthopedics. The mechanism of the signaling 
      pathway has been studied in depth. However, the role of epigenetic regulation, 
      such as lncRNA, is still far from clear. In this study, we successfully 
      established a rat model of DDH and demonstrated that H19 was down-regulated in 
      the development of DDH. Further, we constructed H19 knockdown (KD) and 
      overexpression chondrocytes. H19 KD suppressed the proliferation of normal 
      chondrocytes, while overexpression of H19 promoted cell proliferation of DDH 
      chondrocytes. Finally, we revealed that H19 bound to let-7 and inhibited its 
      function, acting as a competing endogenous RNA. Down-regulation of H19 is closely 
      associated with DDH progression and H19 is an important epigenetic factor that 
      regulates the proliferation of chondrocytes. H19 may thus be a potential clinical 
      marker for DDH diagnosis and treatment.
FAU - Ning, Bo
AU  - Ning B
AD  - Department of Pediatric Orthopaedics, Children's Hospital of Anhui Medical 
      university, Hefei, Anhui, China. sunjun507@126.com.
FAU - Jin, Rui
AU  - Jin R
FAU - Wang, Dahui
AU  - Wang D
FAU - Sun, Jun
AU  - Sun J
LA  - eng
PT  - Journal Article
DEP - 20190110
PL  - Czech Republic
TA  - Physiol Res
JT  - Physiological research
JID - 9112413
RN  - 0 (H19 long non-coding RNA)
RN  - 0 (MIRNLET7 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Chondrocytes/physiology
MH  - Epigenesis, Genetic/physiology
MH  - Feedback, Physiological/physiology
MH  - Female
MH  - Hip Dislocation, Congenital/*genetics/*metabolism
MH  - MicroRNAs/*genetics/*metabolism
MH  - Pregnancy
MH  - RNA, Long Noncoding/*genetics/*metabolism
MH  - Rats
MH  - Rats, Wistar
EDAT- 2019/01/11 06:00
MHDA- 2019/11/26 06:00
CRDT- 2019/01/11 06:00
PHST- 2019/01/11 06:00 [pubmed]
PHST- 2019/11/26 06:00 [medline]
PHST- 2019/01/11 06:00 [entrez]
AID - 933920 [pii]
AID - 10.33549/physiolres.933920 [doi]
PST - ppublish
SO  - Physiol Res. 2019 Apr 30;68(2):275-284. doi: 10.33549/physiolres.933920. Epub 
      2019 Jan 10.