PMID- 30629447
OWN - NLM
STAT- MEDLINE
DCOM- 20200528
LR  - 20200528
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 18
IP  - 3
DP  - 2019 Mar 1
TI  - Proteomics Analysis Reveals That Structural Proteins of the Virion Core and 
      Involved in Gene Expression Are the Main Source for HLA Class II Ligands in 
      Vaccinia Virus-Infected Cells.
PG  - 900-911
LID - 10.1021/acs.jproteome.8b00595 [doi]
AB  - Protective cellular and humoral immune responses require previous recognition of 
      viral antigenic peptides complexed with human leukocyte antigen (HLA) class II 
      molecules on the surface of the antigen presenting cells. The HLA class 
      II-restricted immune response is important for the control and the clearance of 
      poxvirus infection including vaccinia virus (VACV), the vaccine used in the 
      worldwide eradication of smallpox. In this study, a mass spectrometry analysis 
      was used to identify VACV ligands bound to HLA-DR and -DP class II molecules 
      present on the surface of VACV-infected cells. Twenty-six naturally processed 
      viral ligands among the tens of thousands of cell peptides bound to HLA class II 
      proteins were identified. These viral ligands arose from 19 parental VACV 
      proteins: A4, A5, A18, A35, A38, B5, B13, D1, D5, D7, D12, D13, E3, E8, H5, I2, 
      I3, J2, and K2. The majority of these VACV proteins yielded one HLA ligand and 
      were generated mainly, but not exclusively, by the classical HLA class II antigen 
      processing pathway. Medium-sized and abundant proteins from the virion core 
      and/or involved in the viral gene expression were the major source of VACV 
      ligands bound to HLA-DR and -DP class II molecules. These findings will help to 
      understand the effectiveness of current poxvirus-based vaccines and will be 
      important in the design of new ones.
FAU - Lorente, Elena
AU  - Lorente E
FAU - Martin-Galiano, Antonio J
AU  - Martin-Galiano AJ
FAU - Barnea, Eilon
AU  - Barnea E
AD  - Department of Biology , Technion-Israel Institute of Technology , 32000 Haifa , 
      Israel.
FAU - Barriga, Alejandro
AU  - Barriga A
FAU - Palomo, Concepcion
AU  - Palomo C
FAU - Garcia-Arriaza, Juan
AU  - Garcia-Arriaza J
AD  - Department of Molecular and Cellular Biology , Centro Nacional de Biotecnologia , 
      Consejo Superior de Investigaciones Cientificas (CSIC), 28049 Madrid , Spain.
FAU - Mir, Carmen
AU  - Mir C
FAU - Lauzurica, Pilar
AU  - Lauzurica P
FAU - Esteban, Mariano
AU  - Esteban M
AD  - Department of Molecular and Cellular Biology , Centro Nacional de Biotecnologia , 
      Consejo Superior de Investigaciones Cientificas (CSIC), 28049 Madrid , Spain.
FAU - Admon, Arie
AU  - Admon A
AD  - Department of Biology , Technion-Israel Institute of Technology , 32000 Haifa , 
      Israel.
FAU - Lopez, Daniel
AU  - Lopez D
AUID- ORCID: 0000-0003-0268-5878
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190122
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Ligands)
RN  - 0 (Viral Proteins)
RN  - 0 (Viral Structural Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Cells, Cultured
MH  - Gene Expression
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Humans
MH  - *Ligands
MH  - Mass Spectrometry
MH  - Poxviridae/immunology
MH  - Proteomics/*methods
MH  - Vaccinia/immunology
MH  - Vaccinia virus/*chemistry
MH  - Viral Proteins/immunology
MH  - *Viral Structural Proteins/immunology
MH  - Viral Vaccines
MH  - Virion/*chemistry
OTO - NOTNLM
OT  - HLA
OT  - T cells
OT  - antigen processing
OT  - bioinformatics tools
OT  - cellular immune response
OT  - immunoproteomics
OT  - mass spectrometry
OT  - peptides
OT  - vaccine
OT  - virus
EDAT- 2019/01/11 06:00
MHDA- 2020/05/29 06:00
CRDT- 2019/01/11 06:00
PHST- 2019/01/11 06:00 [pubmed]
PHST- 2020/05/29 06:00 [medline]
PHST- 2019/01/11 06:00 [entrez]
AID - 10.1021/acs.jproteome.8b00595 [doi]
PST - ppublish
SO  - J Proteome Res. 2019 Mar 1;18(3):900-911. doi: 10.1021/acs.jproteome.8b00595. 
      Epub 2019 Jan 22.