PMID- 30631350
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1687-708X (Print)
IS  - 1687-7098 (Electronic)
IS  - 1687-708X (Linking)
VI  - 2018
DP  - 2018
TI  - Effects of UVC Irradiation on Growth and Apoptosis of Scedosporium apiospermum 
      and Lomentospora prolificans.
PG  - 3748594
LID - 10.1155/2018/3748594 [doi]
LID - 3748594
AB  - Scedosporium apiospermum and Lomentospora prolificans are important fungal 
      species isolated from immunocompromised patients. Previous studies have 
      demonstrated that these filamentous fungi exist as saprophytes in the soil and 
      showed the highest minimum inhibitory concentration to several drugs. We aimed to 
      examine how UVC affects the S. apiospermum and L. prolificans by investigating 
      the role of UVC on growth, induction of apoptosis by ethidium bromide 
      (EB)/acridine orange (AO) staining, and transcriptomic study of caspase 
      recruitment domain family, member 9 (CARD-9) gene. Our studies showed that 15 
      minutes of exposure to UVC light effectively increased reduction in both 
      organisms and caused changes in colony morphology, color, and hyphal growth 
      pattern. After 15 min of UVC irradiation, apoptotic cells were quantitated by 
      EB/AO staining, and the percentage of apoptosis was 96.06% in S. apiospermum and 
      28.30% in L. prolificans. CARD-9 gene expression results confirmed that apoptosis 
      was induced in S. apiospermum and L. prolificans after UVC treatment and that S. 
      apiospermum showed a higher expression of apoptosis signaling than L. 
      prolificans. Our study explored the effects of UVC in the inactivation of S. 
      apiospermum and L. prolificans. We hope that our data is useful to other 
      researchers in future studies.
FAU - Muangkaew, Watcharamat
AU  - Muangkaew W
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand.
FAU - Suwanmanee, San
AU  - Suwanmanee S
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand.
FAU - Singkum, Pantira
AU  - Singkum P
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand.
FAU - Pumeesat, Potjaman
AU  - Pumeesat P
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand.
FAU - Luplertlop, Natthanej
AU  - Luplertlop N
AUID- ORCID: 0000-0001-7964-5298
AD  - Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol 
      University, Bangkok, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20181202
PL  - Egypt
TA  - Interdiscip Perspect Infect Dis
JT  - Interdisciplinary perspectives on infectious diseases
JID - 101496545
PMC - PMC6304556
EDAT- 2019/01/12 06:00
MHDA- 2019/01/12 06:01
PMCR- 2018/12/02
CRDT- 2019/01/12 06:00
PHST- 2018/08/04 00:00 [received]
PHST- 2018/11/21 00:00 [accepted]
PHST- 2019/01/12 06:00 [entrez]
PHST- 2019/01/12 06:00 [pubmed]
PHST- 2019/01/12 06:01 [medline]
PHST- 2018/12/02 00:00 [pmc-release]
AID - 10.1155/2018/3748594 [doi]
PST - epublish
SO  - Interdiscip Perspect Infect Dis. 2018 Dec 2;2018:3748594. doi: 
      10.1155/2018/3748594. eCollection 2018.