PMID- 30635013
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 1479-5876 (Electronic)
IS  - 1479-5876 (Linking)
VI  - 17
IP  - 1
DP  - 2019 Jan 11
TI  - Single cell sequencing reveals gene expression signatures associated with bone 
      marrow stromal cell subpopulations and time in culture.
PG  - 23
LID - 10.1186/s12967-018-1766-2 [doi]
LID - 23
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs) are a heterogeneous population that 
      participates in wound healing, immune modulation and tissue regeneration. Next 
      generation sequencing was used to analyze transcripts from single BMSCs in order 
      to better characterize BMSC subpopulations. METHODS: Cryopreserved passage 2 
      BMSCs from one healthy subject were cultured through passage 10. The 
      transcriptomes of bulk BMSCs from designated passages were analyzed with 
      microarrays and RNA sequencing (RNA-Seq). For some passages, single BMSCs were 
      separated using microfluidics and their transcriptomes were analyzed by RNA-Seq. 
      RESULTS: Transcriptome analysis by microarray and RNA-Seq of unseparated BMSCs 
      from passages 2, 4, 6, 8, 9 and 10 yielded similar results; both data sets 
      grouped passages 4 and 6 and passages 9 and 10 together and genes differentially 
      expressed among these early and late passage BMSCs were similar. 3D Diffusion map 
      visualization of single BMSCs from passages 3, 4, 6, 8 and 9 clustered passages 3 
      and 9 into two distinct groups, but there was considerable overlap for passages 
      4, 6 and 8 cells. Markers for early passage, FGFR2, and late passage BMSCs, PLAT, 
      were able to identify three subpopulations within passage 3 BMSCs; one that 
      expressed high levels of FGFR2 and low levels of PLAT; one that expressed low 
      levels of FGFR2 and high levels of PLAT and one that expressed intermediate 
      levels of FGFR2 and low levels of PLAT. CONCLUSIONS: Single BMSCs can be 
      separated by microfluidics and their transcriptome analyzed by next generation 
      sequencing. Single cell analysis of early passage BMSCs identified a 
      subpopulation of cells expressing high levels of FGFR2 that might include 
      skeletal stem cells.
FAU - Liu, Shutong
AU  - Liu S
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
FAU - Stroncek, David F
AU  - Stroncek DF
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA. dstroncek@cc.nih.gov.
FAU - Zhao, Yingdong
AU  - Zhao Y
AD  - Biometric Research Program, Division of Cancer Treatment and Diagnosis, National 
      Cancer Institute, NIH, Bethesda, MD, USA.
FAU - Chen, Victoria
AU  - Chen V
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
FAU - Shi, Rongye
AU  - Shi R
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
FAU - Chen, Jinguo
AU  - Chen J
AD  - Center for Human Immunology, Autoimmunity and Inflammation, National Institute of 
      Allergy and Infectious Diseases, NIH, Bethesda, MD, USA.
FAU - Ren, Jiaqiang
AU  - Ren J
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
FAU - Liu, Hui
AU  - Liu H
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
FAU - Bae, Hee Joon
AU  - Bae HJ
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
FAU - Highfill, Steven L
AU  - Highfill SL
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
FAU - Jin, Ping
AU  - Jin P
AD  - Cell Processing Section, Department of Transfusion Medicine, Clinical Center, 
      National Institutes of Health (NIH), 10 Center Drive-MSC-1184, Building 10, Room 
      3C720, Bethesda, MD, 20892-1184, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
DEP - 20190111
PL  - England
TA  - J Transl Med
JT  - Journal of translational medicine
JID - 101190741
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/metabolism
MH  - Cells, Cultured
MH  - Cluster Analysis
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - Mesenchymal Stem Cells/*metabolism
MH  - *Sequence Analysis, RNA
MH  - *Single-Cell Analysis
MH  - Time Factors
MH  - Transcriptome/genetics
PMC - PMC6330466
OTO - NOTNLM
OT  - Bone marrow stromal cells
OT  - FGFR2
OT  - Next generation sequencing
OT  - PLAT
OT  - Single cell next generation sequencing
EDAT- 2019/01/13 06:00
MHDA- 2020/04/09 06:00
PMCR- 2019/01/11
CRDT- 2019/01/13 06:00
PHST- 2018/11/06 00:00 [received]
PHST- 2018/12/31 00:00 [accepted]
PHST- 2019/01/13 06:00 [entrez]
PHST- 2019/01/13 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/01/11 00:00 [pmc-release]
AID - 10.1186/s12967-018-1766-2 [pii]
AID - 1766 [pii]
AID - 10.1186/s12967-018-1766-2 [doi]
PST - epublish
SO  - J Transl Med. 2019 Jan 11;17(1):23. doi: 10.1186/s12967-018-1766-2.