PMID- 30638362
OWN - NLM
STAT- MEDLINE
DCOM- 20190604
LR  - 20190604
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 11
IP  - 5
DP  - 2019 Feb 6
TI  - Astrocyte Viability and Functionality in Spatially Confined Microcavitation Zone.
PG  - 4889-4899
LID - 10.1021/acsami.8b21410 [doi]
AB  - Blast-induced traumatic brain injury (bTBI) can result in cell/tissue damage and 
      lead to clinical and neuropsychiatric symptoms. Shock waves from a blast 
      propagate through the brain and initiate cascades of mechanical and physiological 
      events that can adversely affect the brain function. Although studies using 
      animal models and brain slices have shown macroscale changes in the brain tissue 
      in response to blast, systematic elucidation of coupling mechanisms is currently 
      lacking. One mechanism that has been postulated and demonstrated repeatedly is 
      the blast-induced generation and subsequent collapse of micron-size bubbles 
      (i.e., microcavitation). Using a custom-designed exposure system, we have 
      previously reported that upon collapsing of microbubbles, astrocytes exhibited 
      changes in the cell viability, cellular biomechanics, production of reactive 
      oxygen species, and activation of apoptotic signaling pathways. In this paper, we 
      have applied microfabrication techniques and seeded astrocytes in a spatially 
      controlled manner to determine the extent of cell damage from the site of the 
      collapse of microbubbles. Such a novel experimental design is proven to 
      facilitate our effort to examine the altered cell viability and functionality by 
      monitoring the transient calcium spiking activity in real-time. We now report 
      that the effect of microcavitation depends on the distance from which cells are 
      seeded, and the cell functionality assessed by calcium dynamics is significantly 
      diminished in the cells located within  approximately 800 mum of the collapsing microbubbles. 
      Both calcium influx across the cell membrane via N-type calcium channels and 
      intracellular calcium store are altered in response to microcavitation. Finally, 
      the FDA-approved poloxamer 188 (P188) was used to reconstitute the compromised 
      cell membrane and restore the cell's reparative capability. This finding may lead 
      to a feasible treatment for partially mitigating the tissue damage associated 
      with bTBI.
FAU - Chen, Bo
AU  - Chen B
AD  - Department of Bioengineering , University of Texas at Arlington , Arlington , 
      Texas 76019 , United States.
FAU - Tjahja, Jessica
AU  - Tjahja J
AD  - Department of Bioengineering , University of Texas at Arlington , Arlington , 
      Texas 76019 , United States.
FAU - Malla, Sameep
AU  - Malla S
AD  - Department of Bioengineering , University of Texas at Arlington , Arlington , 
      Texas 76019 , United States.
FAU - Liebman, Caleb
AU  - Liebman C
AD  - Department of Bioengineering , University of Texas at Arlington , Arlington , 
      Texas 76019 , United States.
FAU - Cho, Michael
AU  - Cho M
AUID- ORCID: 0000-0001-5695-7037
AD  - Department of Bioengineering , University of Texas at Arlington , Arlington , 
      Texas 76019 , United States.
LA  - eng
PT  - Journal Article
DEP - 20190125
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 106392-12-5 (Poloxamer)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - *Astrocytes/cytology/physiology/radiation effects
MH  - Blast Injuries/*physiopathology
MH  - Brain Injuries, Traumatic/*physiopathology
MH  - Calcium/metabolism
MH  - Calcium Signaling/physiology/radiation effects
MH  - Cell Line
MH  - *Cell Survival/physiology/radiation effects
MH  - Cytological Techniques
MH  - High-Energy Shock Waves
MH  - Mice
MH  - Microbubbles
MH  - *Models, Biological
MH  - Particle Size
MH  - Poloxamer/chemistry
OTO - NOTNLM
OT  - astrocytes
OT  - blast-induced traumatic brain injury (bTBI)
OT  - calcium dynamics
OT  - microcavitation
OT  - microfabrication
OT  - poloxamer P188
EDAT- 2019/01/15 06:00
MHDA- 2019/06/05 06:00
CRDT- 2019/01/15 06:00
PHST- 2019/01/15 06:00 [pubmed]
PHST- 2019/06/05 06:00 [medline]
PHST- 2019/01/15 06:00 [entrez]
AID - 10.1021/acsami.8b21410 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2019 Feb 6;11(5):4889-4899. doi: 
      10.1021/acsami.8b21410. Epub 2019 Jan 25.