PMID- 30639397
OWN - NLM
STAT- MEDLINE
DCOM- 20190910
LR  - 20190910
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 698
DP  - 2019 Apr 17
TI  - Acetylcholine suppresses the increase of glia fibrillary acidic protein 
      expression via acetylcholine receptors in cAMP-induced astrocytic differentiation 
      of rat C6 glioma cells.
PG  - 146-153
LID - S0304-3940(19)30026-6 [pii]
LID - 10.1016/j.neulet.2019.01.020 [doi]
AB  - Astrocytes, the most common glial cells in the central nervous system, maintain 
      neuronal functions and have roles in neurological diseases. Acetylcholine (ACh) 
      is one of the most essential neurotransmitters, and ACh receptor (AChR) ligands 
      were recently reported to influence astrocyte functions. However, the functions 
      of ACh, the only endogenous agonist of AChR, in astrocytogenesis and in the 
      expression of astrocytic marker genes have not been known. We previously 
      demonstrated that the inhibition of acetylcholine esterase (AChE) suppressed the 
      differentiation of rat glioma C6 cells, an astrocyte differentiation model, and 
      we observed a suppressive effect of ACh agonists on astrocyte differentiation. 
      Our present study revealed that in the cAMP-induced differentiation of C6 cells, 
      an AChR antagonist alleviated the expression of glia fibrillary acidic protein 
      (GFAP) that had been suppressed by dichlorvos (DDVP), an organophosphate and an 
      AChE inhibitor. Our findings also demonstrated a direct effect of ACh on the GFAP 
      expression, and that muscarinic AChR is involved in the suppressive effect of ACh 
      on the GFAP expression in differentiation-induced C6 cells. This is the first 
      report indicating that ACh the only endogenous agonist for AChRs functions as a 
      mediator of astrocyte differentiation.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Ozawa, Aisa
AU  - Ozawa A
AD  - Laboratory of Anatomy II, Department of Veterinary Medicine, School of Veterinary 
      Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 
      252-5201, Japan. Electronic address: a-ozawa@azabu-u.ac.jp.
FAU - Kadowaki, Erina
AU  - Kadowaki E
AD  - Laboratory of Anatomy II, Department of Veterinary Medicine, School of Veterinary 
      Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 
      252-5201, Japan.
FAU - Horio, Tomoyo
AU  - Horio T
AD  - Laboratory of Anatomy II, Department of Veterinary Medicine, School of Veterinary 
      Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 
      252-5201, Japan.
FAU - Sakaue, Motoharu
AU  - Sakaue M
AD  - Laboratory of Anatomy II, Department of Veterinary Medicine, School of Veterinary 
      Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara, Kanagawa, 
      252-5201, Japan. Electronic address: sakaue@azabu-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190109
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Cholinergic Agents)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Receptors, Cholinergic)
RN  - E0399OZS9N (Cyclic AMP)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/metabolism/*pharmacology
MH  - Animals
MH  - Astrocytes/*drug effects/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Line, Tumor
MH  - Cholinergic Agents/pharmacology
MH  - Cyclic AMP/metabolism
MH  - Glial Fibrillary Acidic Protein/*metabolism
MH  - Glioma/drug therapy/metabolism
MH  - Intermediate Filaments/metabolism
MH  - Neuroglia/drug effects/metabolism
MH  - Rats
MH  - Receptors, Cholinergic/*drug effects/metabolism
OTO - NOTNLM
OT  - Acethylcholine
OT  - Astrocyte
OT  - Differentiation
OT  - GFAP
OT  - Muscarinic acetylcholine receptor
OT  - Prolifelation
EDAT- 2019/01/15 06:00
MHDA- 2019/09/11 06:00
CRDT- 2019/01/15 06:00
PHST- 2018/08/24 00:00 [received]
PHST- 2018/12/10 00:00 [revised]
PHST- 2019/01/09 00:00 [accepted]
PHST- 2019/01/15 06:00 [pubmed]
PHST- 2019/09/11 06:00 [medline]
PHST- 2019/01/15 06:00 [entrez]
AID - S0304-3940(19)30026-6 [pii]
AID - 10.1016/j.neulet.2019.01.020 [doi]
PST - ppublish
SO  - Neurosci Lett. 2019 Apr 17;698:146-153. doi: 10.1016/j.neulet.2019.01.020. Epub 
      2019 Jan 9.