PMID- 30639448
OWN - NLM
STAT- MEDLINE
DCOM- 20200615
LR  - 20200615
IS  - 1873-6300 (Electronic)
IS  - 0891-0618 (Linking)
VI  - 96
DP  - 2019 Mar
TI  - Transient receptor potential melastatin-3 in the rat sensory ganglia of the 
      trigeminal, glossopharyngeal and vagus nerves.
PG  - 116-125
LID - S0891-0618(18)30155-8 [pii]
LID - 10.1016/j.jchemneu.2019.01.005 [doi]
AB  - Transient receptor potential melastatin-3 (TRPM3) is a nonselective cation 
      channel, has permeability of Ca(2+), and probably participates in thermosensitive 
      nociception. In this study, immunohistochemistry for TRPM3 was conducted in the 
      rat trigeminal, glossopharyngeal and vagal sensory ganglia. 
      TRPM3-immunoreactivity was expressed by half of sensory neurons in the trigeminal 
      (TG), petrosal (PG) and jugular ganglia (JG), and by about 80% of sensory neurons 
      in the nodose ganglion (NG). They mostly had small to medium-sized cell bodies. A 
      trichrome immunofluorescence method showed co-existence of TRPM3 with TRP 
      vanilloid 1 (TRPV1) and calcitonin gene-related peptide (CGRP). Approximately 70% 
      of TRPM3-immunoreactive (-IR) neurons contained TRPV1-immunoreactivity in all the 
      examined ganglia. More than 40% of TRPM3-IR neurons exhibited 
      CGRP-immunoreactivity in the TG, PG and JG. Only a few sensory neurons 
      co-expressed TRPM3- and CGRP-immunoreactivity in the NG. In addition, more than 
      40% of TRPM3-IR neurons bound to isolectin B4 in all the examined ganglia. By 
      combination of retrograde tracing method and immunohistochemistry, half of TG 
      neurons innervating the facial skin and incisive papilla expressed 
      TRPM3-immunoreactivity whereas approximately 20% of those innervating the tooth 
      pulp contained TRPM3-immunoreactivity. Co-expression of TRPM3-immunoreactivity 
      with TRPV1- or CGRP-immunoreactivity was common among cutaneous and papillary TG 
      neurons but not among pulpal TG neurons. More than 60% of PG and JG neurons 
      innervating the external ear canal skin and circumvallate papilla contained 
      TRPM3-immunoreactivity. Co-expression of TRPM3 with TRPV1 or CGRP was common 
      among PG and JG neurons innervating the external ear canal skin. However, a 
      smaller number of TRPM3-IR neurons co-expressing TRPV1- or CGRP-immunoreactivity 
      innervate the circumvallate papilla in the PG. The present study suggests that 
      expression of TRPM3 and its co-existence with TRPV1 and CGRP in sensory neurons 
      depend on the variety of their peripheral targets in the trigeminal, 
      glossopharyngeal and vagal nervous systems.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Yajima, Takehiro
AU  - Yajima T
AD  - Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku 
      University, Sendai 980-8575, Japan; Liaison Center for Innovative Dentistry, 
      Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan. 
      Electronic address: t.yajima@dent.tohoku.ac.jp.
FAU - Sato, Tadasu
AU  - Sato T
AD  - Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku 
      University, Sendai 980-8575, Japan.
FAU - Shimazaki, Kenichiro
AU  - Shimazaki K
AD  - Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku 
      University, Sendai 980-8575, Japan.
FAU - Ichikawa, Hiroyuki
AU  - Ichikawa H
AD  - Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku 
      University, Sendai 980-8575, Japan.
LA  - eng
PT  - Journal Article
DEP - 20190110
PL  - Netherlands
TA  - J Chem Neuroanat
JT  - Journal of chemical neuroanatomy
JID - 8902615
RN  - 0 (TRPM Cation Channels)
RN  - 0 (TRPM3 protein, rat)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (Trpv1 protein, rat)
RN  - JHB2QIZ69Z (Calcitonin Gene-Related Peptide)
SB  - IM
MH  - Animals
MH  - Calcitonin Gene-Related Peptide/metabolism
MH  - Face/*innervation
MH  - Ganglia, Sensory/cytology/*metabolism
MH  - Male
MH  - Nociception/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - TRPM Cation Channels/*metabolism
MH  - TRPV Cation Channels/metabolism
OTO - NOTNLM
OT  - CGRP
OT  - Immunohistochemistry
OT  - Rat
OT  - Sensory ganglion
OT  - TRPM3
OT  - TRPV1
EDAT- 2019/01/15 06:00
MHDA- 2020/06/17 06:00
CRDT- 2019/01/15 06:00
PHST- 2018/09/20 00:00 [received]
PHST- 2019/01/08 00:00 [revised]
PHST- 2019/01/09 00:00 [accepted]
PHST- 2019/01/15 06:00 [pubmed]
PHST- 2020/06/17 06:00 [medline]
PHST- 2019/01/15 06:00 [entrez]
AID - S0891-0618(18)30155-8 [pii]
AID - 10.1016/j.jchemneu.2019.01.005 [doi]
PST - ppublish
SO  - J Chem Neuroanat. 2019 Mar;96:116-125. doi: 10.1016/j.jchemneu.2019.01.005. Epub 
      2019 Jan 10.