PMID- 30640558 OWN - NLM STAT- MEDLINE DCOM- 20200629 LR - 20200629 IS - 1479-487X (Electronic) IS - 0959-3330 (Linking) VI - 41 IP - 18 DP - 2020 Jul TI - A novel dicationic Quinoline-Carzole fluorescent probe: preparation and labelling of Microthrix parvicella. PG - 2393-2399 LID - 10.1080/09593330.2019.1567606 [doi] AB - A novel dicationic Quinoline-Carbazole fluorescent probe with hydrophobic long-chain alkane was designed and synthesized based on the property of Microthrix parvicella (M. parvicella) in situ utilizing long-chain fatty acids (LCFA) in the activated sludge system. (1)H NMR spectrum, ultraviolet-visible (UV-Vis) absorption spectra, and fluorescent spectra analysis demonstrated that the probe was successfully obtained. The probe had a large stokes-shift ranging from 102 to 144 nm in different solvents, which were benefit for the fluorescent labelling properties. The labelling experiment indicated that the prepared probe could absorb onto the surface of M. parvicella through hydrophobic bond. Much stronger yellow fluorescence of M. parvicella was observed at the concentration of 1.0 x 10(-5 )mol/L when compared with the zooglea, which makes it easy to distinguish M. parvicella from the zooglea. In addition, the photostability of the probe was also investigated, and the result showed that the probe was quite stable in a long period of time. All the results indicated that the prepared probe was suitable for the labelling of M. parvicella. FAU - Fei, Xuening AU - Fei X AD - School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, People's Republic of China. AD - Tianjin Engineering Technology Center of Chemical Wastewater Source Reduction and Recycling, Tianjin Chengjian University, Tianjin, People's Republic of China. AD - School of Science, Tianjin Chengjian University, Tianjin, People's Republic of China. AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, People's Republic of China. FAU - Xing, Yanjun AU - Xing Y AD - School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, People's Republic of China. FAU - Zhang, Buqing AU - Zhang B AD - School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin, People's Republic of China. FAU - Zhu, Sen AU - Zhu S AD - Tianjin Engineering Technology Center of Chemical Wastewater Source Reduction and Recycling, Tianjin Chengjian University, Tianjin, People's Republic of China. AD - School of Science, Tianjin Chengjian University, Tianjin, People's Republic of China. FAU - Liu, Lijuan AU - Liu L AD - Tianjin Engineering Technology Center of Chemical Wastewater Source Reduction and Recycling, Tianjin Chengjian University, Tianjin, People's Republic of China. AD - School of Science, Tianjin Chengjian University, Tianjin, People's Republic of China. LA - eng PT - Journal Article DEP - 20190130 PL - England TA - Environ Technol JT - Environmental technology JID - 9884939 RN - 0 (Fluorescent Dyes) RN - 0 (Quinolines) RN - 0 (Sewage) SB - IM MH - *Actinobacteria MH - Fluorescent Dyes MH - Hydrophobic and Hydrophilic Interactions MH - *Quinolines MH - Sewage OTO - NOTNLM OT - Microthrix parvicella OT - Characterization OT - Dicationic Quinoline-Carbazole OT - fluorescent probe OT - labelling EDAT- 2019/01/15 06:00 MHDA- 2020/07/01 06:00 CRDT- 2019/01/15 06:00 PHST- 2019/01/15 06:00 [pubmed] PHST- 2020/07/01 06:00 [medline] PHST- 2019/01/15 06:00 [entrez] AID - 10.1080/09593330.2019.1567606 [doi] PST - ppublish SO - Environ Technol. 2020 Jul;41(18):2393-2399. doi: 10.1080/09593330.2019.1567606. Epub 2019 Jan 30.