PMID- 30642008
OWN - NLM
STAT- MEDLINE
DCOM- 20190401
LR  - 20200225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 2
DP  - 2019 Jan 11
TI  - Anti-Allergic and Anti-Inflammatory Effects of Kuwanon G and Morusin on MC/9 Mast 
      Cells and HaCaT Keratinocytes.
LID - 10.3390/molecules24020265 [doi]
LID - 265
AB  - Atopic dermatitis (AD) is a prevalent chronic inflammatory skin disease. The use 
      of immunomodulatory corticosteroids in AD treatment causes adverse side effects. 
      Therefore, novel natural anti-inflammatory therapeutics are needed. The aim of 
      the present study was to investigate the anti-allergic and anti-inflammatory 
      activities of kuwanon G and morusin. To investigate the effect of kuwanon G and 
      morusin on skin inflammation, enzyme-linked immunosorbent assays (ELISA) to 
      quantitate secreted (RANTES/CCL5), thymus- and activation-regulated chemokine 
      (TARC/CCL17), and macrophage-derived chemokine (MDC/CCL22) were performed, 
      followed by Western blotting to measure the phosphorylation of signal transducer 
      and activator of transcription 1 (STAT1) and nuclear transcription factor-kappaB 
      (NF-kappaB) p65 in tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma 
      (IFN-gamma)-stimulated HaCaT keratinocytes. In order to evaluate the anti-allergic 
      effects, ELISA to quantify histamine and leukotriene C(4) (LTC(4)) production and 
      Western blotting to measure 5-lipoxygenase (5-LO) activation were performed using 
      PMA and A23187-stimulated MC/9 mast cells. Kuwanon G reduced the release of 
      RANTES/CCL5, TARC/CCL17, and MDC/CCL22 via down-regulation of STAT1 and NF-kappaB p65 
      signaling in TNF-alpha and IFN-gamma-stimulated HaCaT keratinocytes. Kuwanon G also 
      inhibited histamine production and 5-LO activation in PMA and A23187-stimulated 
      MC/9 mast cells. Morusin inhibited RANTES/CCL5 and TARC/CCL17 secretion via the 
      suppression of STAT1 and NF-kappaB p65 phosphorylation in TNF-alpha and IFN-gamma-stimulated 
      HaCaT keratinocytes, and the release of histamine and LTC(4) by suppressing 5-LO 
      activation in PMA and A23187-stimulated MC/9 mast cells. Kuwanon G and morusin 
      are potential anti-inflammatory mediators for the treatment of allergic and 
      inflammatory skin diseases such as AD.
FAU - Jin, Seong Eun
AU  - Jin SE
AD  - Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 
      Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea. noellajin@kiom.re.kr.
FAU - Ha, Hyekyung
AU  - Ha H
AUID- ORCID: 0000-0002-7326-6366
AD  - Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 
      Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea. hkha@kiom.re.kr.
FAU - Shin, Hyeun-Kyoo
AU  - Shin HK
AD  - Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 
      Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea. hkshin@kiom.re.kr.
FAU - Seo, Chang-Seob
AU  - Seo CS
AUID- ORCID: 0000-0002-8156-446X
AD  - Herbal Medicine Research Division, Korea Institute of Oriental Medicine, 1672 
      Yuseong-daero, Yuseong-gu, Daejeon 34054, Korea. csseo0914@kiom.re.kr.
LA  - eng
GR  - K17251/Korea Institute of Oriental Medicine/
GR  - K18241/Korea Institute of Oriental Medicine/
PT  - Journal Article
DEP - 20190111
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Anti-Allergic Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Biomarkers)
RN  - 0 (Chemokines)
RN  - 0 (Flavonoids)
RN  - 0 (NF-kappa B)
RN  - 0 (STAT1 Transcription Factor)
RN  - 0 (STAT1 protein, human)
RN  - 62596-29-6 (morusin)
RN  - 75629-19-5 (kuwanon G)
SB  - IM
MH  - Anti-Allergic Agents/chemistry/*pharmacology
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Biomarkers
MH  - Cell Line
MH  - Chemokines/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Flavonoids/chemistry/*pharmacology
MH  - Humans
MH  - Keratinocytes/*drug effects/*metabolism
MH  - Mast Cells/*drug effects/*metabolism
MH  - Molecular Structure
MH  - NF-kappa B/metabolism
MH  - Phosphorylation
MH  - STAT1 Transcription Factor/metabolism
PMC - PMC6359505
OTO - NOTNLM
OT  - Kuwanon G
OT  - anti-allergy
OT  - anti-skin inflammation
OT  - morusin
COIS- The authors declare no conflict of interest.
EDAT- 2019/01/16 06:00
MHDA- 2019/04/02 06:00
PMCR- 2019/01/11
CRDT- 2019/01/16 06:00
PHST- 2018/11/22 00:00 [received]
PHST- 2018/12/24 00:00 [revised]
PHST- 2019/01/09 00:00 [accepted]
PHST- 2019/01/16 06:00 [entrez]
PHST- 2019/01/16 06:00 [pubmed]
PHST- 2019/04/02 06:00 [medline]
PHST- 2019/01/11 00:00 [pmc-release]
AID - molecules24020265 [pii]
AID - molecules-24-00265 [pii]
AID - 10.3390/molecules24020265 [doi]
PST - epublish
SO  - Molecules. 2019 Jan 11;24(2):265. doi: 10.3390/molecules24020265.