PMID- 30644104
OWN - NLM
STAT- MEDLINE
DCOM- 20191212
LR  - 20191217
IS  - 1943-3670 (Electronic)
IS  - 0022-3492 (Linking)
VI  - 90
IP  - 7
DP  - 2019 Jul
TI  - Periodontal ligament-derived mesenchymal stem cells modulate neutrophil responses 
      via paracrine mechanisms.
PG  - 747-755
LID - 10.1002/JPER.18-0220 [doi]
AB  - BACKGROUND: Mesenchymal stem cells differentiate into distinct mesenchymal cell 
      lineages and regulate the immune response. The aim of this study was to determine 
      whether periodontal ligament-derived mesenchymal stem cells (PDLSCs) have the 
      ability to modulate neutrophil responses via paracrine mechanisms. METHODS: 
      CD105-enriched PDLSCs were seeded for 24 h and challenged with Porphyromonas 
      gingivalis total protein extract (PgPE) (0 or 2 ug/mL) for 3 h. Cells were then 
      washed and further cultured for 18 h and the supernatants were collected and 
      stored. Next, neutrophil-differentiated human promyelocytic leukemia HL-60 cells 
      (HL60D) were treated with PDLSCs supernatants and HL-60D activation and 
      functional responses were determined. RESULTS: PgPE treatment induced higher 
      secretion of inflammatory markers and chemokines by PDLSCs, including RANTES, 
      eotaxin, interferon (IFN)-gamma- inducible protein 10 (IP-10), monocyte 
      chemoattractant protein-1 (MCP-1), IFN-gamma, interleukin (IL)-6, IL-8 and IL-1ra 
      (P < 0.05). HL-60D recruitment rate was increased by 4.7 +/- 1.09-fold when exposed 
      to PgPE-treated PDLSCs supernatants. PgPE-treated PDLSCs supernatants promoted a 
      1.78 +/- 1.04-fold increase in the production of intracellular reactive oxygen 
      species (ROS) by PMA-stimulated HL-60D, whereas PgPE-untreated PDLSCs 
      supernatants led to a 16% reduction in intracellular ROS. In sharp contrast, 
      neither PgPE-untreated nor PgPE-treated PDLSCs supernatants altered tumor 
      necrosis factor (TNF)-alpha and IL-1beta secretion by HL-60D cells. CONCLUSION: 
      Together, these findings suggest an important role of PDLSCs in the recognition 
      of P. gingivalis, paracrine recruitment and activation of antimicrobial 
      mechanisms in innate immune cells, without interfering in cytokine responses.
CI  - (c) 2019 American Academy of Periodontology.
FAU - Misawa, Monica Yuri Orita
AU  - Misawa MYO
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
FAU - Silverio Ruiz, Karina Gonzales
AU  - Silverio Ruiz KG
AD  - Division of Periodontics, Department of Prosthodontics and Periodontics, 
      Piracicaba Dental School, University of Campinas, Piracicaba, Sao Paulo, Brazil.
FAU - Nociti, Francisco Humberto Jr
AU  - Nociti FH Jr
AD  - Division of Periodontics, Department of Prosthodontics and Periodontics, 
      Piracicaba Dental School, University of Campinas, Piracicaba, Sao Paulo, Brazil.
FAU - Albiero, Mayra Laino
AU  - Albiero ML
AD  - Division of Periodontics, Department of Prosthodontics and Periodontics, 
      Piracicaba Dental School, University of Campinas, Piracicaba, Sao Paulo, Brazil.
FAU - Saito, Miki Taketomi
AU  - Saito MT
AD  - Division of Periodontics, Department of Prosthodontics and Periodontics, 
      Piracicaba Dental School, University of Campinas, Piracicaba, Sao Paulo, Brazil.
FAU - Nobrega Stipp, Rafael
AU  - Nobrega Stipp R
AD  - Department of Oral Diagnosis, Piracicaba Dental School, University of Campinas, 
      Piracicaba, Sao Paulo, Brazil.
FAU - Condino-Neto, Antonio
AU  - Condino-Neto A
AD  - Department of Immunology, Institute of Biomedical Sciences, University of Sao 
      Paulo, Sao Paulo, Sao Paulo, Brazil.
FAU - Holzhausen, Marinella
AU  - Holzhausen M
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
FAU - Palombo, Henrique
AU  - Palombo H
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
FAU - Villar, Cristina Cunha
AU  - Villar CC
AD  - Division of Periodontics, Department of Stomatology, School of Dentistry, 
      University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil.
AD  - Department of Periodontics, UTHSC, San Antonio Dental School, San Antonio, TX, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190212
PL  - United States
TA  - J Periodontol
JT  - Journal of periodontology
JID - 8000345
SB  - IM
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - Neutrophils
MH  - Osteogenesis
MH  - *Periodontal Ligament
OTO - NOTNLM
OT  - cellular biology
OT  - inflammation
OT  - innate immune responses
OT  - neutrophils
OT  - reactive oxygen species
EDAT- 2019/01/16 06:00
MHDA- 2019/12/18 06:00
CRDT- 2019/01/16 06:00
PHST- 2018/04/09 00:00 [received]
PHST- 2018/11/03 00:00 [revised]
PHST- 2018/11/06 00:00 [accepted]
PHST- 2019/01/16 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/01/16 06:00 [entrez]
AID - 10.1002/JPER.18-0220 [doi]
PST - ppublish
SO  - J Periodontol. 2019 Jul;90(7):747-755. doi: 10.1002/JPER.18-0220. Epub 2019 Feb 
      12.