PMID- 30644238 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220331 IS - 2008-076X (Print) IS - 2008-0778 (Electronic) IS - 2008-0778 (Linking) VI - 13 IP - 1 DP - 2019 Apr TI - The Association between TNF-alpha Gene Polymorphisms and Endometriosis in An Iranian Population. PG - 6-11 LID - 10.22074/ijfs.2019.5542 [doi] AB - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) is an important cytokine in acute inflammatory response to infective factors. Based on investigation in different populations, it is thought that this response increases in patients with endometriosis due to the presence of cytokines such as TNF-alpha. This study aimed to examine the association of four TNF-alpha polymorphisms, namely -238G/A, -308G/A, -857C/T and -863C/A, with susceptibility to endometriosis in an Iranian population. MATERIALS AND METHODS: We recruited 150 women with endometriosis and 150 women without endometriosis in this case-control study and collected 4 ml of blood from all subjects. After DNA extraction, the polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: The allele frequency of TNF-alpha -863C/A in the case and control groups showed a significant difference [odds ratios (OR)=0.64, 95% confidence interval (CI)=0.41-0.99, P=0.047] but the result is not significant when Adjusting for multiple testing (P=0.188). No significant difference in the allele frequencies of -238G/A (OR=1.07, 95% CI=0.51-2.25, P=0.862), -308G/A (OR=0.79, 95% CI=0.43-1.45, P=0.438) and -857C/T (OR=1.03, 95% CI=0.66- 1.61, P=0.887) was observed. We adjusted all four polymorphism genotypes by age and body mass index (BMI), however, no significant difference was detected. There was an association between the case and control and BMI when adjusting by age (OR=1.082, 95% CI=1.009-1.162, P=0.028). CONCLUSION: For the first time the association of the four polymorphisms in the promoter region of the TNF-alpha gene with endometriosis has been conducted in women of Iranian origin. The present research reveals the -863 A allele may play a role in incidence of endometriosis among Iranian women. Development of endometriosis among those people with -863 A allele seems low. According to the results, the current study indicates that there might be a correlation between BMI and progression of endometriosis. CI - Copyright(c) by Royan Institute. All rights reserved. FAU - Babaabasi, Babak AU - Babaabasi B AD - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. AD - Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran. FAU - Ahani, Ali AU - Ahani A AD - Mendel Medical Genetics Laboratory, Tehran, Iran. Electronic Address: ali.ahani@gmail.com. FAU - Sadeghi, Faegheh AU - Sadeghi F AD - Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran. FAU - Bashizade-Fakhar, Haniyeh AU - Bashizade-Fakhar H AD - Department of Laboratory Science, Chalous Branch, Islamic Azad University, Chalous, Iran. FAU - Khorram Khorshid, Hamid Reza AU - Khorram Khorshid HR AD - Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran. LA - eng PT - Journal Article DEP - 20190106 PL - Iran TA - Int J Fertil Steril JT - International journal of fertility & sterility JID - 101487941 PMC - PMC6334017 OTO - NOTNLM OT - Body Mass Index OT - Endometriosis OT - Polymorphisms OT - Restriction Fragment Length Polymorphism OT - TNF-alpha COIS- The authors declare no conflicts of interest. EDAT- 2019/01/16 06:00 MHDA- 2019/01/16 06:01 PMCR- 2019/04/01 CRDT- 2019/01/16 06:00 PHST- 2018/04/21 00:00 [received] PHST- 2018/07/04 00:00 [accepted] PHST- 2019/01/16 06:00 [entrez] PHST- 2019/01/16 06:00 [pubmed] PHST- 2019/01/16 06:01 [medline] PHST- 2019/04/01 00:00 [pmc-release] AID - 10.22074/ijfs.2019.5542 [doi] PST - ppublish SO - Int J Fertil Steril. 2019 Apr;13(1):6-11. doi: 10.22074/ijfs.2019.5542. Epub 2019 Jan 6.