PMID- 30644486
OWN - NLM
STAT- MEDLINE
DCOM- 20190906
LR  - 20190906
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 19
IP  - 4
DP  - 2019 Feb 12
TI  - A microfluidic sensor for detecting chlorophenols using cross-linked enzyme 
      aggregates (CLEAs).
PG  - 634-640
LID - 10.1039/c8lc01065j [doi]
AB  - Chlorophenols have a strong medicinal smell and can be detected by the human nose 
      at parts-per-million levels. Therefore, continuous monitoring of chlorophenols in 
      water supplies is highly important. Herein, we reported a microfluidic sensor 
      which can be used to detect 2,4-dichlorophenol (2,4-DCP) in real time with a 
      limit of detection of around 0.1 ppm. The microfluidic sensor is a membrane-less 
      galvanic cell which consists of two laminar flows running in parallel inside a 
      straight channel. The sensor measures the potential difference between a solution 
      containing 2,4-DCP and a reference solution containing acetate buffer. In a 
      continuous-flow mode, the cell potential is proportional to the concentration of 
      2,4-DCP. To render specificity for the sensor, we incorporate a pre-treatment 
      section where the incoming solution containing 2,4-DCP is split into two streams. 
      One of the streams is brought into contact with cross-linked laccase aggregates 
      (which catalyzes the hydrolysis of 2,4-DCP) and the second stream is taken as a 
      reference solution. By comparing the potential difference between the two 
      streams, we can determine the concentration of 2,4-DCP with high specificity. The 
      microfluidic sensor platform is potentially useful for real-time detection of 
      micropollutants present in aquatic systems with high sensitivity and specificity.
FAU - Ho, Wing Fat
AU  - Ho WF
AD  - Department of Chemical and Biomolecular Engineering, National University of 
      Singapore, 4 Engineering Drive 4, 117585 Singapore. cheyk@nus.edu.sg.
FAU - Nguyen, Le Truc
AU  - Nguyen LT
FAU - Yang, Kun-Lin
AU  - Yang KL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Chlorophenols)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Protein Aggregates)
RN  - EC 1.10.3.2 (Laccase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Chlorophenols/*analysis/metabolism
MH  - Electrodes
MH  - Enzymes, Immobilized/chemistry/metabolism
MH  - Laccase/chemistry/*metabolism
MH  - *Microfluidic Analytical Techniques/instrumentation
MH  - Oxidation-Reduction
MH  - Particle Size
MH  - Protein Aggregates
MH  - Surface Properties
MH  - Trametes/enzymology
EDAT- 2019/01/16 06:00
MHDA- 2019/09/07 06:00
CRDT- 2019/01/16 06:00
PHST- 2019/01/16 06:00 [pubmed]
PHST- 2019/09/07 06:00 [medline]
PHST- 2019/01/16 06:00 [entrez]
AID - 10.1039/c8lc01065j [doi]
PST - ppublish
SO  - Lab Chip. 2019 Feb 12;19(4):634-640. doi: 10.1039/c8lc01065j.