PMID- 30644695
OWN - NLM
STAT- MEDLINE
DCOM- 20190912
LR  - 20190912
IS  - 1735-1502 (Print)
IS  - 1735-1502 (Linking)
VI  - 17
IP  - 6
DP  - 2018 Dec 2
TI  - Elevated Expression of Tim-3 and PD-1 Immune Checkpoint Receptors on T-CD4+ 
      Lymphocytes of Patients with Asthma.
PG  - 517-525
AB  - Asthma is a chronic disorder of the airways characterized by reversible airflow 
      obstruction, inflammation and bronchial hyperresponsiveness. Different immune 
      cells and molecules have been attributed to involve in pathogenesis of asthma. In 
      the current case-control study, the expression of T cell Ig and mucin 
      domain-containing molecule-3 (Tim-3) and programmed death-1 (PD-1) was studied on 
      CD4+ T cells of patients with asthma and normal controls. The frequency of 
      Tim-3+/PD-1+/CD4+ T cells was determined by a three color flow cytometry method 
      in 37 patients with asthma and 32 healthy controls. To evaluate the Th1/Th2 
      ratio, peripheral blood mononuclear cells were isolated from all samples and 
      stimulated with phorbol 12- myristate 13- acetate ( PMA)/ionomycin for 18 h. 
      IFN-gamma) and Interleukin-4 (IL-4) were measured in culture supernatants by-(ELISA). 
      Serum total immunoglobulin E (IgE) was also measured in all samples. Significant 
      increase in percentage and absolute count of Tim-3+/PD-1+/CD4+, Tim-3+/CD4+ and 
      PD-1+/CD4+ T cells was found in asthmatic patients compared to healthy controls 
      (p=0.02 and p=0.003, respectively). The IFN-gamma/IL-4 ratio (Th1/Th2 ratio) was 
      significantly higher in healthy controls than that of asthmatic patients 
      (p=0.029). Our data regarding the increased expression of PD-1 and Tim-3 on CD4+ 
      T cells of patients with asthma suggest the potential roles of these immune 
      checkpoint receptors in immune dys-regulation of asthma.
FAU - Mosayebian, Ali
AU  - Mosayebian A
AD  - Department of Immunology, School of Medicine, Mazandaran University of Medical 
      Sciences, Sari, Iran. ali.mosayebian@gmail.com.
FAU - Koohini, Zohreh
AU  - Koohini Z
AD  - Department of Immunology, School of Medicine, Mazandaran University of Medical 
      Sciences, Sari, Iran. z.koohini@gmail.com.
FAU - Hossein-Nataj, Hadi
AU  - Hossein-Nataj H
AD  - Department of Immunology, School of Medicine, Mazandaran University of Medical 
      Sciences, Sari, Iran. hadi.nataj2000@gmail.com.
FAU - Abediankenari, Saeid
AU  - Abediankenari S
AD  - Department of Immunology, School of Medicine, Mazandaran University of Medical 
      Sciences, Sari, Iran AND Immunogenetics Research Center, School of Medicine, 
      Mazandaran University of Medical Sciences, Sari, Iran. abedianlab@yahoo.co.uk.
FAU - Abedi, Siavash
AU  - Abedi S
AD  - Department of Internal Medicine, Pulmonary and Critical Care Division, Mazandaran 
      University of Medical Sciences, Sari, Iran. siavash.abedi0@gmail.com.
FAU - Asgarian-Omran, Hossein
AU  - Asgarian-Omran H
AD  - Department of Immunology, School of Medicine, Mazandaran University of Medical 
      Sciences, Sari, Iran AND Immunogenetics Research Center, School of Medicine, 
      Mazandaran University of Medical Sciences, Sari, Iran. asgarianhossein@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20181202
PL  - Iran
TA  - Iran J Allergy Asthma Immunol
JT  - Iranian journal of allergy, asthma, and immunology
JID - 101146178
RN  - 0 (HAVCR2 protein, human)
RN  - 0 (Hepatitis A Virus Cellular Receptor 2)
RN  - 0 (IL4 protein, human)
RN  - 0 (PDCD1 protein, human)
RN  - 0 (Programmed Cell Death 1 Receptor)
RN  - 207137-56-2 (Interleukin-4)
SB  - IM
MH  - Adult
MH  - Asthma/*immunology
MH  - CD4-Positive T-Lymphocytes/*metabolism
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Female
MH  - Flow Cytometry
MH  - Hepatitis A Virus Cellular Receptor 2/*metabolism
MH  - Humans
MH  - Immunophenotyping
MH  - Interleukin-4/metabolism
MH  - Male
MH  - Middle Aged
MH  - Programmed Cell Death 1 Receptor/*metabolism
MH  - Up-Regulation
OTO - NOTNLM
OT  - Asthma
OT  - Interferon-gamma
OT  - Interleukin-4
OT  - PD-1
OT  - Tim-3
EDAT- 2019/01/16 06:00
MHDA- 2019/09/13 06:00
CRDT- 2019/01/16 06:00
PHST- 2017/12/04 00:00 [received]
PHST- 2018/05/01 00:00 [accepted]
PHST- 2019/01/16 06:00 [entrez]
PHST- 2019/01/16 06:00 [pubmed]
PHST- 2019/09/13 06:00 [medline]
PST - epublish
SO  - Iran J Allergy Asthma Immunol. 2018 Dec 2;17(6):517-525.