PMID- 30648255
OWN - NLM
STAT- MEDLINE
DCOM- 20200526
LR  - 20221207
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 234
IP  - 8
DP  - 2019 Aug
TI  - The Smad2/3/4 complex binds miR-139 promoter to modulate TGFbeta-induced 
      proliferation and activation of human Tenon's capsule fibroblasts through the Wnt 
      pathway.
PG  - 13342-13352
LID - 10.1002/jcp.28011 [doi]
AB  - The activation and proliferation of human Tenon's fibroblasts (HTFs) play a vital 
      role in the fibrosis in the pathology of the scar formation after the glaucoma 
      filtration surgery. Transforming growth factor beta1 (TGFbeta1)/Smads signaling has 
      been reported to promote fibrosis. In our previous study, we revealed that 
      TGFbeta1-induced orbital fibroblast activation and proliferation through 
      Wnt/beta-catenin signaling. As microRNA (miR)-139 could target several factors in 
      Wnt signaling to modulate fibrosis, here, the effect and mechanism of miR-139 in 
      HTF activation and proliferation were investigated. miR-139 overexpression 
      significantly reversed the TGFbeta1-induced increase in collagen I and alpha-smooth 
      muscle actin contents and proliferation in HTFs. CTNNB1 and CTNND1 were direct 
      downstream of miR-139 and can significantly restore the suppressive effect of 
      miR-139 on the activation and proliferation in HTFs under TGFbeta1 stimulation. 
      Smad2/3/4 complex inhibits the transcription activity of miR-139, most possibly 
      by Smad4 binding to the miR-139 promoter. Taken together, we demonstrated a new 
      mechanism of HTF activation and proliferation from the perspective of miRNA 
      regulation, which may provide new strategies for improving the fibrosis after the 
      glaucoma filtration surgery.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Deng, Mi
AU  - Deng M
AD  - Department of Ophthalmology, Hunan Clinical Research Center of Ophthalmic 
      Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 
      China.
FAU - Hou, Shi-Ying
AU  - Hou SY
AD  - Department of Ophthalmology, Hunan Clinical Research Center of Ophthalmic 
      Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 
      China.
FAU - Tong, Bo-Ding
AU  - Tong BD
AD  - Department of Ophthalmology, Hunan Clinical Research Center of Ophthalmic 
      Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 
      China.
FAU - Yin, Jia-Yang
AU  - Yin JY
AD  - Department of Ophthalmology, Hunan Clinical Research Center of Ophthalmic 
      Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 
      China.
FAU - Xiong, Wei
AU  - Xiong W
AUID- ORCID: 0000-0002-3017-9895
AD  - Department of Ophthalmology, Hunan Clinical Research Center of Ophthalmic 
      Disease, The Second Xiangya Hospital, Central South University, Changsha, Hunan, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190115
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (Catenins)
RN  - 0 (MIRN139 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (SMAD2 protein, human)
RN  - 0 (SMAD3 protein, human)
RN  - 0 (SMAD4 protein, human)
RN  - 0 (Smad Proteins)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad3 Protein)
RN  - 0 (Smad4 Protein)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (beta Catenin)
RN  - 0 (Delta Catenin)
SB  - IM
MH  - Binding Sites/genetics
MH  - Catenins/antagonists & inhibitors/genetics/metabolism
MH  - Cell Proliferation
MH  - Cicatrix/etiology/metabolism/pathology
MH  - Fibroblasts/cytology/metabolism
MH  - Fibrosis
MH  - Glaucoma Drainage Implants/adverse effects
MH  - Humans
MH  - MicroRNAs/*genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - Smad Proteins/*metabolism
MH  - Smad2 Protein/metabolism
MH  - Smad3 Protein/metabolism
MH  - Smad4 Protein/metabolism
MH  - Tenon Capsule/*cytology/*metabolism
MH  - Transforming Growth Factor beta1/metabolism
MH  - Wnt Signaling Pathway
MH  - beta Catenin/antagonists & inhibitors/genetics/metabolism
MH  - Delta Catenin
OTO - NOTNLM
OT  - Smad2/3/4 complex
OT  - TGFbeta1
OT  - human Tenon's fibroblasts (HTFs)
OT  - miR-139
OT  - proliferation
EDAT- 2019/01/17 06:00
MHDA- 2020/05/27 06:00
CRDT- 2019/01/17 06:00
PHST- 2018/11/02 00:00 [received]
PHST- 2018/12/11 00:00 [accepted]
PHST- 2019/01/17 06:00 [pubmed]
PHST- 2020/05/27 06:00 [medline]
PHST- 2019/01/17 06:00 [entrez]
AID - 10.1002/jcp.28011 [doi]
PST - ppublish
SO  - J Cell Physiol. 2019 Aug;234(8):13342-13352. doi: 10.1002/jcp.28011. Epub 2019 
      Jan 15.