PMID- 30648577
OWN - NLM
STAT- MEDLINE
DCOM- 20200323
LR  - 20220809
IS  - 2224-6614 (Electronic)
IS  - 1021-9498 (Print)
VI  - 27
IP  - 1
DP  - 2019 Jan
TI  - Identification of baicalin from Bofutsushosan and Daisaikoto as a potent inducer 
      of glucose uptake and modulator of insulin signaling-associated pathways.
PG  - 240-248
LID - 10.1016/j.jfda.2018.07.002 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by 
      hyperglycemia that can lead to long-term complications including heart diseases, 
      stroke, retinopathy, and renal failure. Treatment strategies include stimulating 
      glucose uptake and controlling blood glucose level. Bofutsushosan (BOF) and 
      Daisaikoto (DAI) are two herb-based kampo medicines that have been demonstrated 
      to improve metabolism-associated disorders including obesity, hyperlipidemia, and 
      nonalcoholic fatty liver. Given their bioactivities against metabolic syndromes, 
      we explored in this study the effect of BOF and DAI extracts on glucose 
      absorption and used them as source to identify phytochemical stimulator of 
      glucose absorption. Glucose uptake and mechanistic studies were evaluated in 
      differentiated C2C12 skeletal muscle cells, and HPLC analysis was used to 
      determine the molecular bioactive constituents. Our results indicated that the 
      ethanolic extracts of BOF and DAI (BOFEE and DAIEE, respectively) enhanced the 
      glucose uptake ratio in the differentiated C2C12 cells, and further analysis 
      identified the flavone baicalin as a major constituent capable of efficiently 
      stimulating glucose absorption. Mechanistic studies revealed that the effect from 
      baicalin involved the activation of IRS-1 and GLUT-4, and implicated the AMPK, 
      PI3K/Akt, and MAPK/ERK signaling cascades. Due to its potency, we suggest that 
      baicalin merit further evaluation as a potential candidate anti-hyperglycemic 
      agent for the treatment and management of T2DM.
CI  - Copyright (c) 2018. Published by Elsevier Taiwan LLC.
FAU - Kuo, Yu-Ting
AU  - Kuo YT
AD  - Department of Medical Imaging, Chi Mei Medical Center, No. 901 Zhonghua Road, 
      Yongkang District, Tainan, 710, Taiwan.
FAU - Lin, Chih-Chan
AU  - Lin CC
AD  - Department of Medical Research, Chi Mei Medical Center, No. 901 Zhonghua Road, 
      Yongkang District, Tainan, 710, Taiwan.
FAU - Kuo, Hsiao-Tzu
AU  - Kuo HT
AD  - Department of Nutritional Health, Chia-Nan University of Pharmacy and Science, 60 
      Erh-Jen 1st Road, Jen-Te Hsiang, Tainan, 717, Taiwan.
FAU - Hung, Jui-Hsiang
AU  - Hung JH
AD  - Department of Biotechnology, Chia-Nan University of Pharmacy and Science, 60 
      Erh-Jen 1st Road, Jen-Te Hsiang, Tainan, 717, Taiwan.
FAU - Liu, Ching-Hsuan
AU  - Liu CH
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, No. 250, Wu-Hsing Street, Taipei, 11031, Taiwan.
AD  - Department of Microbiology & Immunology, Dalhousie University, 5850 College 
      Street, Halifax, Nova Scotia, B3H 4R2, Canada.
FAU - Jassey, Alagie
AU  - Jassey A
AD  - International Ph.D. Program in Medicine, College of Medicine, Taipei Medical 
      University, No. 250, Wu-Hsing Street, Taipei, 11031, Taiwan.
FAU - Yen, Ming-Hong
AU  - Yen MH
AD  - Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical 
      University, No. 100 Shih-Chuan 1st Road, Kaohsiung, 807, Taiwan.
FAU - Wu, Shu-Jing
AU  - Wu SJ
AD  - Department of Nutritional Health, Chia-Nan University of Pharmacy and Science, 60 
      Erh-Jen 1st Road, Jen-Te Hsiang, Tainan, 717, Taiwan.
FAU - Lin, Liang-Tzung
AU  - Lin LT
AD  - Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical 
      University, No. 250, Wu-Hsing Street, Taipei, 11031, Taiwan.
AD  - Department of Microbiology and Immunology, School of Medicine, College of 
      Medicine, Taipei Medical University, No. 250, Wu-Hsing Street, Taipei, 11031, 
      Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180814
PL  - China (Republic : 1949- )
TA  - J Food Drug Anal
JT  - Journal of food and drug analysis
JID - 101630927
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Flavonoids)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 0 (bofu-tsusho-san)
RN  - 0 (daisaikoto)
RN  - 347Q89U4M5 (baicalin)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Drugs, Chinese Herbal/chemistry/*pharmacology
MH  - Flavonoids/analysis/*pharmacology
MH  - Glucose/*metabolism
MH  - Glucose Transporter Type 4/genetics/metabolism
MH  - Hypoglycemic Agents/chemistry/*pharmacology
MH  - Insulin/*metabolism
MH  - Mice
MH  - Phosphatidylinositol 3-Kinases/genetics/metabolism
MH  - Signal Transduction/drug effects
PMC - PMC9298638
OTO - NOTNLM
OT  - Anti-hyperglycemia
OT  - Baicalin
OT  - Bofutsushosan
OT  - Daisaikoto
OT  - Glucose uptake
COIS- Conflicts of interest The authors declared that no competing interests exist.
EDAT- 2019/01/17 06:00
MHDA- 2020/03/24 06:00
PMCR- 2018/08/14
CRDT- 2019/01/17 06:00
PHST- 2018/04/11 00:00 [received]
PHST- 2018/06/20 00:00 [revised]
PHST- 2018/07/19 00:00 [accepted]
PHST- 2019/01/17 06:00 [entrez]
PHST- 2019/01/17 06:00 [pubmed]
PHST- 2020/03/24 06:00 [medline]
PHST- 2018/08/14 00:00 [pmc-release]
AID - j.jfda.2018.07.002 [pii]
AID - jfda-27-01-240 [pii]
AID - 10.1016/j.jfda.2018.07.002 [doi]
PST - ppublish
SO  - J Food Drug Anal. 2019 Jan;27(1):240-248. doi: 10.1016/j.jfda.2018.07.002. Epub 
      2018 Aug 14.