PMID- 30650519
OWN - NLM
STAT- MEDLINE
DCOM- 20190606
LR  - 20200309
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 11
IP  - 1
DP  - 2019 Jan 15
TI  - Differential Role of Anti-Viral Sensing Pathway for the Production of Type I 
      Interferon beta in Dendritic Cells and Macrophages Against Respiratory Syncytial 
      Virus A2 Strain Infection.
LID - 10.3390/v11010062 [doi]
LID - 62
AB  - Respiratory syncytial virus (RSV) is a major cause of respiratory infectious 
      disease in infants and young children. Dendritic cells (DCs) and macrophages 
      (MACs) are known to play important roles in RSV recognition, and in the 
      production of type I interferons (IFNs) and pro-inflammatory cytokine in RSV 
      infection. Toll-like receptor 7 (TLR7), myeloid differentiation primary response 
      88 (MyD88), and mitochondrial antiviral-signaling protein (MAVS) are known to be 
      important for the RSV sensing pathway in DCs and MACs. However, despite the 
      critical roles of type I IFNs in the anti-RSV immune response, the pattern 
      recognition receptors (PRRs) that are required for RSV sensing in DCs and MACs 
      remain unclear. Here, we investigate the pathway activated by RSV A2 strain 
      infection using an IFN-beta/YFP reporter mouse model to visualize IFN-beta-producing 
      cells and in vitro RSV infection in bone marrow-derived DCs (BM-DCs) and 
      macrophages (BM-DMs). We present our finding that MyD88, but not TLR7, are 
      important for RSV recognition and type I IFN and pro-inflammatory production in 
      DCs and MACs. MAVS-deficient BM-DCs and BM-DMs show impaired induction of IFN-beta 
      production upon RSV stimulation, and this effect is RSV replication-dependent. 
      Our study provides information on cell type-specific PRR requirements in innate 
      immune responses against RSV infection.
FAU - Oh, Dong Sun
AU  - Oh DS
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology (KAIST), Daejeon 34141, Republic of Korea. 
      dongsun.oh@kaist.ac.kr.
FAU - Kim, Tae Hoon
AU  - Kim TH
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology (KAIST), Daejeon 34141, Republic of Korea. 
      thkim21@cha.ac.kr.
AD  - Department of Internal Medicine, CHA Bundang Medical Center, CHA University, 
      Seongnam 13496, Republic of Korea. thkim21@cha.ac.kr.
FAU - Lee, Heung Kyu
AU  - Lee HK
AUID- ORCID: 0000-0002-3977-1510
AD  - Graduate School of Medical Science and Engineering, Korea Advanced Institute of 
      Science and Technology (KAIST), Daejeon 34141, Republic of Korea. 
      heungkyu.lee@kaist.ac.kr.
AD  - KAIST Institute for Health Science and Technology, KAIST, Daejeon 34141, Republic 
      of Korea. heungkyu.lee@kaist.ac.kr.
LA  - eng
GR  - NRF-2016R1A2B2015028/National Research Foundation of Korea/International
GR  - NRF-2018M3A9H3024611/National Research Foundation of Korea/International
GR  - 2018R1A6A3A01012960/National Research Foundation of Korea/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190115
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Pattern Recognition)
RN  - 0 (Tlr7 protein, mouse)
RN  - 0 (Toll-Like Receptor 7)
RN  - 77238-31-4 (Interferon-beta)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology/virology
MH  - *Immunity, Innate
MH  - Interferon-beta/*immunology
MH  - Macrophages/*immunology/virology
MH  - Membrane Glycoproteins/immunology
MH  - Mice
MH  - Myeloid Differentiation Factor 88/immunology
MH  - Receptors, Pattern Recognition
MH  - Respiratory Syncytial Virus, Human/*immunology
MH  - *Signal Transduction
MH  - Toll-Like Receptor 7/immunology
MH  - Virus Replication
PMC - PMC6356365
OTO - NOTNLM
OT  - Dendritic cell
OT  - MAVS
OT  - Macrophage
OT  - MyD88
OT  - Respiratory syncytial virus
OT  - TLR7
OT  - Type I interferon
COIS- The authors declare no conflicts of interest.
EDAT- 2019/01/18 06:00
MHDA- 2019/06/07 06:00
PMCR- 2019/01/01
CRDT- 2019/01/18 06:00
PHST- 2018/12/18 00:00 [received]
PHST- 2019/01/10 00:00 [revised]
PHST- 2019/01/12 00:00 [accepted]
PHST- 2019/01/18 06:00 [entrez]
PHST- 2019/01/18 06:00 [pubmed]
PHST- 2019/06/07 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - v11010062 [pii]
AID - viruses-11-00062 [pii]
AID - 10.3390/v11010062 [doi]
PST - epublish
SO  - Viruses. 2019 Jan 15;11(1):62. doi: 10.3390/v11010062.