PMID- 30651353
OWN - NLM
STAT- MEDLINE
DCOM- 20190530
LR  - 20231006
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 294
IP  - 11
DP  - 2019 Mar 15
TI  - The kinetic mechanisms of fast-decay red-fluorescent genetically encoded calcium 
      indicators.
PG  - 3934-3946
LID - 10.1074/jbc.RA118.004543 [doi]
AB  - Genetically encoded calcium indicators (GECIs) are useful reporters of 
      cell-signaling, neuronal, and network activities. We have generated novel fast 
      variants and investigated the kinetic mechanisms of two recently developed 
      red-fluorescent GECIs (RGECIs), mApple-based jRGECO1a and mRuby-based jRCaMP1a. 
      In the formation of fluorescent jRGECO1a and jRCaMP1a complexes, calcium binding 
      is followed by rate-limiting isomerization. However, fluorescence decay of 
      calcium-bound jRGECO1a follows a different pathway from its formation: 
      dissociation of calcium occurs first, followed by the peptide, similarly to 
      GCaMP-s. In contrast, fluorescence decay of calcium-bound jRCaMP1a occurs by the 
      reversal of the on-pathway: peptide dissociation is followed by calcium. The 
      mechanistic differences explain the generally slower off-kinetics of 
      jRCaMP1a-type indicators compared with GCaMP-s and jRGECO1a-type GECI: the 
      fluorescence decay rate of f-RCaMP1 was 21 s(-1), compared with 109 s(-1) for 
      f-RGECO1 and f-RGECO2 (37  degrees C). Thus, the CaM-peptide interface is an important 
      determinant of the kinetic responses of GECIs; however, the topology of the 
      structural link to the fluorescent protein demonstrably affects the internal 
      dynamics of the CaM-peptide complex. In the dendrites of hippocampal CA3 neurons, 
      f-RGECO1 indicates calcium elevation in response to a 100 action potential train 
      in a linear fashion, making the probe particularly useful for monitoring 
      large-amplitude, fast signals, e.g. those in dendrites, muscle cells, and immune 
      cells.
CI  - (c) 2019 Kerruth et al.
FAU - Kerruth, Silke
AU  - Kerruth S
AD  - From the Molecular and Clinical Sciences Research Institute, St. George's, 
      University of London, London SW17 0RE, United Kingdom and.
FAU - Coates, Catherine
AU  - Coates C
AD  - From the Molecular and Clinical Sciences Research Institute, St. George's, 
      University of London, London SW17 0RE, United Kingdom and.
FAU - Durst, Celine D
AU  - Durst CD
AD  - the Institute for Synaptic Physiology, Center for Molecular Neurobiology Hamburg, 
      20251 Hamburg, Germany.
FAU - Oertner, Thomas G
AU  - Oertner TG
AD  - the Institute for Synaptic Physiology, Center for Molecular Neurobiology Hamburg, 
      20251 Hamburg, Germany.
FAU - Torok, Katalin
AU  - Torok K
AUID- ORCID: 0000-0002-3107-4534
AD  - From the Molecular and Clinical Sciences Research Institute, St. George's, 
      University of London, London SW17 0RE, United Kingdom and k.torok@sgul.ac.uk.
LA  - eng
SI  - PDB/3U0K
SI  - PDB/4I2Y
SI  - PDB/3WLD
GR  - BB/M02556X/1/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190116
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Anthraquinones)
RN  - 0 (nuclear fast red)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Anthraquinones/chemistry/*metabolism
MH  - Calcium/*analysis/*metabolism
MH  - *Calcium Signaling
MH  - HEK293 Cells
MH  - Humans
MH  - Kinetics
MH  - Models, Molecular
PMC - PMC6422079
OTO - NOTNLM
OT  - biosensor
OT  - calcium
OT  - calcium imaging
OT  - fluorescence
OT  - kinetics
COIS- The authors declare that they have no conflicts of interest with the contents of 
      this article
EDAT- 2019/01/18 06:00
MHDA- 2019/05/31 06:00
PMCR- 2019/01/16
CRDT- 2019/01/18 06:00
PHST- 2018/06/20 00:00 [received]
PHST- 2019/01/14 00:00 [revised]
PHST- 2019/01/18 06:00 [pubmed]
PHST- 2019/05/31 06:00 [medline]
PHST- 2019/01/18 06:00 [entrez]
PHST- 2019/01/16 00:00 [pmc-release]
AID - S0021-9258(20)41807-1 [pii]
AID - RA118.004543 [pii]
AID - 10.1074/jbc.RA118.004543 [doi]
PST - ppublish
SO  - J Biol Chem. 2019 Mar 15;294(11):3934-3946. doi: 10.1074/jbc.RA118.004543. Epub 
      2019 Jan 16.