PMID- 30652078
OWN - NLM
STAT- MEDLINE
DCOM- 20200219
LR  - 20200309
IS  - 2211-5463 (Print)
IS  - 2211-5463 (Electronic)
IS  - 2211-5463 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Jan
TI  - Heat induces myogenic transcription factors of myoblast cells via transient 
      receptor potential vanilloid 1 (Trpv1).
PG  - 101-113
LID - 10.1002/2211-5463.12550 [doi]
AB  - Exercise generates heat, blood flow, and metabolic changes, thereby inducing 
      hypertrophy of skeletal muscle cells. However, the mechanism by which heat 
      incudes hypertrophy in response to heat is not well known. Here, we hypothesized 
      that heat would induce differentiation of myoblast cells. We investigated the 
      underlying mechanism by which myoblast cells respond to heat. When mouse myoblast 
      cells were exposed to 42  degrees C for over 30 min, the phosphorylation level of protein 
      kinase C (PKC) and heat shock factor 1 (Hsf1) increased, and the mRNA and protein 
      expression level of heat shock protein 70 (Hsp70) increased. Inhibitors of 
      transient receptor potential vanilloid 1 (Trpv1), calmodulin, PKC, and Hsf1, and 
      the small interfering RNA-mediated knockdown of Trpv1 diminished those heat 
      responses. Heat exposure increased the phosphorylation levels of thymoma viral 
      proto-oncogene 1 (Akt), mammalian target of rapamycin (mTOR), eukaryotic 
      translation initiation factor 4E binding protein 1 (Eif4ebp1), and ribosomal 
      protein S6 kinase, polypeptide 1 (S6K1). The knockdown of Trpv1 decreased these 
      heat-induced responses. Antagonists of Hsp70 inhibited the phosphorylation level 
      of Akt. Finally, heat increased the protein expression level of skeletal muscle 
      markers such as myocyte enhancer factor 2D, myogenic factor 5, myogenic factor 6, 
      and myogenic differentiation 1. Heat also increased myotube formation. Knockdown 
      of Trpv1 diminished heat-induced increases of those proteins and myotube 
      formation. These results indicate that heat induces myogenic transcription 
      factors of myoblast cells through the Trpv1, calmodulin, PKC, Hsf1, Hsp70, Akt, 
      mTOR, Eif4ebp1, and S6K1 pathway. Moreover, heat increases myotube formation 
      through Trpv1.
FAU - Obi, Syotaro
AU  - Obi S
AD  - Research Support Center Dokkyo Medical University Tochigi Japan.
AD  - Department of Cardiovascular Medicine Dokkyo Medical University Tochigi Japan.
FAU - Nakajima, Toshiaki
AU  - Nakajima T
AD  - Department of Cardiovascular Medicine Dokkyo Medical University Tochigi Japan.
AD  - Heart Center Dokkyo Medical University Hospital Tochigi Japan.
FAU - Hasegawa, Takaaki
AU  - Hasegawa T
AD  - Department of Cardiovascular Medicine Dokkyo Medical University Tochigi Japan.
FAU - Nakamura, Fumitaka
AU  - Nakamura F
AD  - Third Department of Internal Medicine Teikyo University Chiba Medical Center 
      Japan.
FAU - Sakuma, Masashi
AU  - Sakuma M
AD  - Department of Cardiovascular Medicine Dokkyo Medical University Tochigi Japan.
FAU - Toyoda, Shigeru
AU  - Toyoda S
AD  - Department of Cardiovascular Medicine Dokkyo Medical University Tochigi Japan.
AD  - Heart Center Dokkyo Medical University Hospital Tochigi Japan.
FAU - Tei, Chuwa
AU  - Tei C
AD  - Department of Cardiovascular Medicine Dokkyo Medical University Tochigi Japan.
FAU - Inoue, Teruo
AU  - Inoue T
AD  - Research Support Center Dokkyo Medical University Tochigi Japan.
AD  - Department of Cardiovascular Medicine Dokkyo Medical University Tochigi Japan.
AD  - Heart Center Dokkyo Medical University Hospital Tochigi Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181203
PL  - England
TA  - FEBS Open Bio
JT  - FEBS open bio
JID - 101580716
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV1 protein, mouse)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *Hot Temperature
MH  - *Muscle Development
MH  - Muscle, Skeletal/metabolism
MH  - Myoblasts/cytology/*metabolism
MH  - Signal Transduction
MH  - TRPV Cation Channels/*metabolism
MH  - Transcription Factors/*metabolism
PMC - PMC6325605
OTO - NOTNLM
OT  - heat response signaling
OT  - skeletal muscle
OT  - translation
EDAT- 2019/01/18 06:00
MHDA- 2019/01/18 06:01
PMCR- 2018/12/03
CRDT- 2019/01/18 06:00
PHST- 2018/04/13 00:00 [received]
PHST- 2018/09/25 00:00 [revised]
PHST- 2018/10/30 00:00 [accepted]
PHST- 2019/01/18 06:00 [entrez]
PHST- 2019/01/18 06:00 [pubmed]
PHST- 2019/01/18 06:01 [medline]
PHST- 2018/12/03 00:00 [pmc-release]
AID - FEB412550 [pii]
AID - 10.1002/2211-5463.12550 [doi]
PST - epublish
SO  - FEBS Open Bio. 2018 Dec 3;9(1):101-113. doi: 10.1002/2211-5463.12550. eCollection 
      2019 Jan.