PMID- 30656598
OWN - NLM
STAT- MEDLINE
DCOM- 20200317
LR  - 20200317
IS  - 1993-0402 (Electronic)
IS  - 1672-0415 (Linking)
VI  - 25
IP  - 9
DP  - 2019 Sep
TI  - Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-kappaB 
      Signaling Pathway.
PG  - 677-683
LID - 10.1007/s11655-018-2916-8 [doi]
AB  - OBJECTIVE: To illustrate the molecular mechanisms underlying the therapeutic 
      effects of electroacupuncture (EA) on knee osteoarthritis (OA). METHODS: 
      Twenty-seven six-month-old New Zealand white rabbits were allocated into three 
      groups in accordance with a random number table: normal group (no surgery-induced 
      OA; without treatment), model group (surgery-induced OA; without treatment) and 
      EA group [surgery-induced OA; received treatment with EA at acupoints Dubi (ST 
      35) and Neixiyan (EX-LE 5), 30 min twice a day]. After eight consecutive weeks of 
      treatment, the histopathological alterations in cartilage were observed using 
      optical microscopy and transmission electron microscopy, cartilage degeneration 
      was evaluated by modified Mankin's score principles, the synovial fluid 
      concentration of interleukin-1beta (IL-1beta), interleukin-6 (IL-6), tumor necrosis 
      factor-alpha (TNF-alpha) and matrix metalloproteinase-3 (MMP-3) were evaluated by 
      enzyme-linked immunosorbent assay, and the protein expression levels of IL-1beta, 
      IL-6, TNF-alpha, MMP-3, IkappaB kinase-beta (IKK-beta), nuclear factor of alpha light polypeptide 
      gene enhancer in B-cells inhibitor alpha (IkappaB-alpha) and nuclear factor-kappaB (NF-kappaB) p65 
      were quantified by Western blot analysis. RESULTS: EA treatment significantly 
      improved cartilage structure arrangement and reduced cellular degeneration. The 
      IL-1beta, IL-6, TNF-alpha and MMP-3 of synovial fluid in the EA-treated group were 
      significantly decreased compared with the model group (all P<0.01). Compared with 
      the model group, the IL-1beta, IL-6, TNF-alpha, MMP-3, IKK-beta and NF-kappaB p65 protein 
      expressions in cartilage of EA-treated group were significantly decreased (all 
      P<0.01), whereas IkappaB-alpha expression was significantly up-regulated (P<0.01). 
      CONCLUSION: EA treatment may delay cartilage degeneration by down-regulating 
      inflammatory factors through NF-kappaB signaling pathway, which may, in part, explain 
      its clinical efficacy in the treatment of knee OA.
FAU - Wu, Guang-Wen
AU  - Wu GW
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, 350122, China.
FAU - Chen, Jun
AU  - Chen J
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, 350122, China.
FAU - Huang, Yun-Mei
AU  - Huang YM
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, 350122, China.
FAU - Pan, Cai-Bin
AU  - Pan CB
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, 350122, China.
FAU - Chen, Wen-Lie
AU  - Chen WL
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, 350122, China.
FAU - Zhang, Shi-Mao
AU  - Zhang SM
AD  - Fujian Xiangxing Electronic Technology Co., Ltd., Ningde, Fujian Province, 
      352300, China.
FAU - Lin, Wei
AU  - Lin W
AD  - Fujian Provincial Key Laboratory of Integrative Medicine on Geriatrics, Fuzhou, 
      350122, China.
FAU - Liu, Xian-Xiang
AU  - Liu XX
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, 350122, China.
FAU - Wu, Ming-Xia
AU  - Wu MX
AD  - Department of Acupuncture, Second Affiliated People's Hospital of Fujian 
      University of Traditional Chinese Medicine, Fuzhou, 350003, China. 
      wumingxiafz@163.com.
LA  - eng
PT  - Journal Article
DEP - 20190117
PL  - China
TA  - Chin J Integr Med
JT  - Chinese journal of integrative medicine
JID - 101181180
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Transcription Factor RelA)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*pathology
MH  - Chondrocytes/pathology/ultrastructure
MH  - *Electroacupuncture
MH  - I-kappa B Kinase/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 3/metabolism
MH  - NF-KappaB Inhibitor alpha/metabolism
MH  - NF-kappa B/*metabolism
MH  - Rabbits
MH  - *Signal Transduction
MH  - Synovial Fluid/metabolism
MH  - Transcription Factor RelA/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - cartilage
OT  - cytokines
OT  - electroacupuncture
OT  - nuclear factor-kappaB signaling pathway
OT  - osteoarthritis
OT  - synovial fluid
EDAT- 2019/01/19 06:00
MHDA- 2020/03/18 06:00
CRDT- 2019/01/19 06:00
PHST- 2018/06/06 00:00 [accepted]
PHST- 2019/01/19 06:00 [pubmed]
PHST- 2020/03/18 06:00 [medline]
PHST- 2019/01/19 06:00 [entrez]
AID - 10.1007/s11655-018-2916-8 [pii]
AID - 10.1007/s11655-018-2916-8 [doi]
PST - ppublish
SO  - Chin J Integr Med. 2019 Sep;25(9):677-683. doi: 10.1007/s11655-018-2916-8. Epub 
      2019 Jan 17.