PMID- 30657547
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20200622
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 1
DP  - 2019 Jan
TI  - Expression of T follicular helper lymphocytes with different subsets and analysis 
      of serum IL-6, IL-17, TGF-beta and MMP-3 contents in patients with rheumatoid 
      arthritis.
PG  - 61-69
LID - 16748 [pii]
LID - 10.26355/eurrev_201901_16748 [doi]
AB  - OBJECTIVE: To investigate the expression levels of T follicular helper (Tfh) with 
      different subsets in patients with rheumatoid arthritis (RA) and their serum 
      interleukin-6 (IL-6), interleukin-17 (IL-17), transforming growth factor-beta 
      (TGF-beta) and matrix metalloproteinase 3 (MMP-3) contents. PATIENTS AND METHODS: 
      The medical records of 45 RA patients in the Department of Rheumatology and 
      Immunology in the First Affiliated Hospital of Chengdu Medical College from 
      January 2016 to April 2018 were retrospectively analyzed. They were divided into 
      the RA high activity group (24 cases, group A) (DAS28 score >/= 5.0) and RA low 
      activity group (21 cases, group B) (3.2 < DAS28 score < 5.0). At the same time, 
      20 healthy subjects were selected as a control group. Flow cytometry was used to 
      detect the expression levels of Tfh1, Tfh2 and Tfh17, enzyme-linked immunosorbent 
      assay to detect serum IL-6, IL-17, IL-21 and MMP-3 concentrations. The 
      correlation of Tfh cells with IL-6, IL-17, IL-21 and MMP-3 was analyzed. RESULTS: 
      Those of peripheral blood mononuclear cell (PBMC) Tfh2 and Tfh17 cells were 
      significantly higher in group A than those in group B (p < 0.05). Compared with 
      the control group, the concentrations of serum IL-6, IL-17 and MMP-3 
      significantly increased (p < 0.001), but that of serum TGF-beta markedly decreased 
      in group A and group B (p < 0.01). The concentrations of serum IL-6, IL-17 and 
      MMP-3 were remarkably higher in group A than those in group B (p < 0.001), but 
      that of serum TGF-beta was significantly lower in group A than that in group B (p < 
      0.001). The expression level of PBMC Tfh2 cells, PBMC Tfh17 cells was positively 
      correlated with serum IL-6, IL-17 and MMP-3. The expression levels of Tfh2 and 
      Tfh17 cells are positively correlated with serum IL-6, IL-17 and MMP-3 
      concentrations, negatively correlated with serum TGF-beta concentration. 
      CONCLUSIONS: Tfh2 and Tfh17 are expected to be new targets for immunotherapy in 
      RA patients.
FAU - Sun, W-K
AU  - Sun WK
AD  - School of Laboratory Medicine/Sichuan Provincial Engineering Laboratory for 
      Prevention and Control Technology of Veterinary Drug Residue in Animal-origin 
      Food, Chengdu Medical College, Chengdu, P.R. China. qgw2tb@163.com.
FAU - Bai, Y
AU  - Bai Y
FAU - Yi, M-M
AU  - Yi MM
FAU - Wu, L-J
AU  - Wu LJ
FAU - Chen, J-L
AU  - Chen JL
FAU - Wu, D-M
AU  - Wu DM
FAU - Wu, H-W
AU  - Wu HW
FAU - Wan, L
AU  - Wan L
FAU - Meng, Y
AU  - Meng Y
FAU - Zhang, Q-L
AU  - Zhang QL
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-6)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Arthritis, Rheumatoid/blood/*immunology
MH  - Case-Control Studies
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Interleukin-17/blood/immunology/metabolism
MH  - Interleukin-6/blood/immunology/metabolism
MH  - Lymphocyte Count
MH  - Male
MH  - Matrix Metalloproteinase 3/blood/immunology/metabolism
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Th17 Cells/*immunology/metabolism
MH  - Th2 Cells/*immunology/metabolism
MH  - Transforming Growth Factor beta/immunology/metabolism
EDAT- 2019/01/19 06:00
MHDA- 2020/06/23 06:00
CRDT- 2019/01/19 06:00
PHST- 2019/01/19 06:00 [entrez]
PHST- 2019/01/19 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
AID - 16748 [pii]
AID - 10.26355/eurrev_201901_16748 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Jan;23(1):61-69. doi: 
      10.26355/eurrev_201901_16748.