PMID- 30660042
OWN - NLM
STAT- MEDLINE
DCOM- 20190904
LR  - 20190904
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1108
DP  - 2019 Feb 15
TI  - Dual liquid-liquid extraction followed by LC-MS/MS method for the simultaneous 
      quantification of melatonin, cortisol, triiodothyronine, thyroxine and 
      testosterone levels in serum: Applications to a photoperiod study in rats.
PG  - 11-16
LID - S1570-0232(18)30708-6 [pii]
LID - 10.1016/j.jchromb.2019.01.002 [doi]
AB  - Hypothalamic Pituitary (PH) axes directly affects the functionality of the 
      thyroid gland, the adrenal gland, and the gonads and their alteration has been 
      related to several pathologies. Therefore, the global analysis of representative 
      hormones from each axis, together with melatonin, would be a very good strategy 
      for therapeutic diagnosis. Hence, an accurate, economic and effective analytical 
      method has been developed and validated for the simultaneous measurement of the 
      melatonin, cortisol, triiodothyronine (T3), thyroxine (T4) and testosterone 
      levels in serum. The protocol consists in two liquid-liquid extractions followed 
      by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis 
      with electrospray ionization in positive mode. The isotopically labelled internal 
      standards melatonin-D(4), cortisol-D(4), l-thyroxine-(13)C(6) and 
      testosterone-(13)C(3) were added to serum samples. Multiple reaction monitoring 
      (MRM) mode was performed to target fragment ions for the hormones and internal 
      standards. Excellent linearity (r(2) >/= 0.993) of this method was observed within 
      the concentration range of 0.004-0.5 ng/mL for melatonin and 0.4-50 ng/mL for 
      cortisol, T3, T4 and testosterone in rat sera. The mean recovery of all compounds 
      ranged from 51.3% to 76.7%. The relative standard deviations (RSDs) of intra-day 
      and inter-day precision were within the acceptable limits of +/-15% at all of the 
      concentrations tested. The method developed here has been successfully applied to 
      study the changes of these hormones induced by the duration of light exposure in 
      rat serum, as a physiological model of HP axes modulation. The results obtained 
      from rat sera showed the suitability of this analytical strategy.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Domenech-Coca, Cristina
AU  - Domenech-Coca C
AD  - Universitat Rovira i Virgili, Department of Biochemistry and Biotechnology, 
      Nutrigenomics Research Group, Tarragona, Spain.
FAU - Marine-Casado, Roger
AU  - Marine-Casado R
AD  - Eurecat, Technology Centre of Catalonia, Technological Unit of Nutrition and 
      Health, Reus, Spain.
FAU - Caimari, Antoni
AU  - Caimari A
AD  - Eurecat, Technology Centre of Catalonia, Technological Unit of Nutrition and 
      Health, Reus, Spain.
FAU - Arola, Lluis
AU  - Arola L
AD  - Universitat Rovira i Virgili, Department of Biochemistry and Biotechnology, 
      Nutrigenomics Research Group, Tarragona, Spain; Eurecat, Technology Centre of 
      Catalonia, Technological Unit of Nutrition and Health, Reus, Spain.
FAU - Del Bas, Josep Maria
AU  - Del Bas JM
AD  - Eurecat, Technology Centre of Catalonia, Technological Unit of Nutrition and 
      Health, Reus, Spain.
FAU - Blade, Cinta
AU  - Blade C
AD  - Universitat Rovira i Virgili, Department of Biochemistry and Biotechnology, 
      Nutrigenomics Research Group, Tarragona, Spain. Electronic address: 
      mariacinta.blade@urv.cat.
FAU - Rodriguez-Naranjo, Maria Isabel
AU  - Rodriguez-Naranjo MI
AD  - Education, Science and Sport Department, Castilla-La Mancha, Spain.
LA  - eng
PT  - Journal Article
DEP - 20190103
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 3XMK78S47O (Testosterone)
RN  - JL5DK93RCL (Melatonin)
RN  - Q51BO43MG4 (Thyroxine)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/methods
MH  - Hydrocortisone/*blood
MH  - Limit of Detection
MH  - Liquid-Liquid Extraction/methods
MH  - Male
MH  - Melatonin/*blood
MH  - Photoperiod
MH  - Rats
MH  - Tandem Mass Spectrometry/methods
MH  - Testosterone/*blood
MH  - Thyroxine/*blood
MH  - Triiodothyronine/*blood
OTO - NOTNLM
OT  - Cortisol
OT  - LC-MS/MS
OT  - Melatonin
OT  - Testosterone
OT  - Thyroxine
OT  - Triiodothyronine
EDAT- 2019/01/20 06:00
MHDA- 2019/09/05 06:00
CRDT- 2019/01/20 06:00
PHST- 2018/05/02 00:00 [received]
PHST- 2018/12/29 00:00 [revised]
PHST- 2019/01/02 00:00 [accepted]
PHST- 2019/01/20 06:00 [pubmed]
PHST- 2019/09/05 06:00 [medline]
PHST- 2019/01/20 06:00 [entrez]
AID - S1570-0232(18)30708-6 [pii]
AID - 10.1016/j.jchromb.2019.01.002 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Feb 15;1108:11-16. doi: 
      10.1016/j.jchromb.2019.01.002. Epub 2019 Jan 3.