PMID- 30660454
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240331
IS  - 0975-9476 (Print)
IS  - 0976-2809 (Electronic)
IS  - 0975-9476 (Linking)
VI  - 12
IP  - 1
DP  - 2021 Jan-Mar
TI  - Scientific validation of anti-arthritic effect of Kashayams - A polyherbal 
      formulation in collagen induced arthritic rats.
PG  - 20-27
LID - S0975-9476(17)30742-8 [pii]
LID - 10.1016/j.jaim.2018.02.139 [doi]
AB  - BACKGROUND: Toll-like receptor-4 (TLR-4) mediates activation of nuclear factor 
      kappa-light-chain-enhancer of activated B cells (NF-kappaB) resulting in induction of 
      proinflammatory genes such as that encoding tumor necrosis factor-alpha (TNF-alpha) and 
      interleukin-1beta (IL-1beta) which played a significant role in cartilage destruction 
      of rheumatoid arthritis (RA). Low risk and better efficacy made herbal drugs more 
      reliable than nonsteroid anti-inflammatory drugs (NSAIDS) in RA treatment. 
      Gugguluthiktam Kashayam (GuK), Punarnavadi Kashayam (PuK) and Balaguluchiadi 
      Kashayam (BgK) are ayurvedic polyherbal formulations prescribed in classical 
      ayurvedic texts Sahasrayogam and Ashtangahridayam as medicines for the treatment 
      of RA. OBJECTIVE: The objective of the present study was to elucidate the 
      molecular mechanism of anti-arthritic effect of these Kashayams on TLR-4 signal 
      transduction pathway in collagen induced arthritic rats. MATERIAL AND METHODS: 
      The wistar rats grouped into group I - Normal, group II- Collagen induced 
      arthritis (CIA), group III- CIA + BgK, group IV- CIA + PuK, group V- CIA + GuK, 
      group VI - CIA + Indomethacin (3 mg/kg b.wt.). Treatment with Kashayam (2 ml/kg 
      b.wt) started after 14 days of primary immunization with type II collagen and 
      continued for a period of 45 days. RESULTS: Arthritis index, C-reactive protein 
      (CRP), rheumatoid factor (RF) and myeloperoxidase (MPO) in serum and protein 
      level of TLR-4, myeloid differentiation factor 88 (MYD88), NF-kappaB, TNF-alpha, IL-1beta, 
      inducible nitric oxide synthase (iNOS), cyclooxygenase-2 COX-2) and prostaglandin 
      E-2 (PGE-2) in cartilage were significantly elevated in CIA rats. Further, 
      treatment with Kashayams downregulated all these inflammatory mediators hitherto 
      TLR-4-NF-kB signal transduction pathway except IL-10, an anti-inflammatory 
      cytokine which showed a reverse effect. CONCLUSION: This molecular mechanism of 
      the investigation confirmed the clinical efficacy of Kashayams in preventing the 
      progression of RA and gave an intuition of the scientific validation of 
      Kashayams, an Ayurvedic classical medicine.
CI  - Copyright (c) 2018 Transdisciplinary University, Bangalore and World Ayurveda 
      Foundation. Published by Elsevier B.V. All rights reserved.
FAU - I S, Aswathy
AU  - I S A
AD  - Department of Biochemistry, University of Kerala, Kariavattom, 
      Thiruvananthapuram, 695581, Kerala, India.
FAU - Krishnan, Santhi
AU  - Krishnan S
AD  - Department of Biochemistry, University of Kerala, Kariavattom, 
      Thiruvananthapuram, 695581, Kerala, India.
FAU - Peter, Jasmine
AU  - Peter J
AD  - Department of Biochemistry, University of Kerala, Kariavattom, 
      Thiruvananthapuram, 695581, Kerala, India.
FAU - Sabu, Vidya
AU  - Sabu V
AD  - Department of Biochemistry, University of Kerala, Kariavattom, 
      Thiruvananthapuram, 695581, Kerala, India.
FAU - Helen, A
AU  - Helen A
AD  - Department of Biochemistry, University of Kerala, Kariavattom, 
      Thiruvananthapuram, 695581, Kerala, India. Electronic address: 
      helenbios@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20190117
PL  - United States
TA  - J Ayurveda Integr Med
JT  - Journal of Ayurveda and integrative medicine
JID - 101551404
PMC - PMC8039352
OTO - NOTNLM
OT  - Anti-Arthritic
OT  - Kashayams
OT  - Nuclear factor kappa-light-chain-enhancer of activated B cells
OT  - Rheumatoid arthritis
OT  - Toll-like receptor -4
OT  - Type II collagen
EDAT- 2019/01/21 06:00
MHDA- 2019/01/21 06:01
PMCR- 2019/01/17
CRDT- 2019/01/21 06:00
PHST- 2017/12/05 00:00 [received]
PHST- 2018/01/11 00:00 [revised]
PHST- 2018/02/02 00:00 [accepted]
PHST- 2019/01/21 06:00 [pubmed]
PHST- 2019/01/21 06:01 [medline]
PHST- 2019/01/21 06:00 [entrez]
PHST- 2019/01/17 00:00 [pmc-release]
AID - S0975-9476(17)30742-8 [pii]
AID - 10.1016/j.jaim.2018.02.139 [doi]
PST - ppublish
SO  - J Ayurveda Integr Med. 2021 Jan-Mar;12(1):20-27. doi: 10.1016/j.jaim.2018.02.139. 
      Epub 2019 Jan 17.