PMID- 30662660
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 10
IP  - 12
DP  - 2018
TI  - Effects of mammalian target of rapamycin on proliferation, apoptosis and 
      differentiation of myoblasts undergoing mechanical stress.
PG  - 4173-4182
AB  - Myoblasts characterize by the potential to transform into skeletal muscle, and 
      involve in processes of proliferation, differentiation and apoptosis. Mammalian 
      target of rapamycin (mTOR) is an important protein of PI3K signaling pathway in 
      muscle metabolism and physiology. This study aimed to investigate effects of mTOR 
      on proliferation, apoptosis and differentiation of myoblasts undergoing 
      mechanical stress. We paid much attention on mTOR function undergoing mechanical 
      stress in myoblasts. C2C12 myoblasts were cultured and mTOR gene was knocked down 
      by using Crisper/Cas9 method. Western blot assay and quantitative polymerase 
      chain reaction (Q-PCR) were used to test 4E-binding protein 1 (4EBP1) and p70 
      ribosomal protein S6 kinase (p70S6k) expression. Cell counting kit 8 (CCK-8) was 
      used to measure cell proliferation, and flow cytometry to used to detect cell 
      apoptosis. Differentiation was counted by using immunofluorescence staining. 
      Rresults showed that the knockdown of mTOR reduced the phosphorylation of 4EBP1 
      and p70S6k levels undergoing mechanical stress and decreased PI3K signaling 
      pathway proteins synthesis. In addition, proliferation of myoblasts was 
      decelerated by the mTOR knockdown. However, when mTOR knocked down cells treated 
      with mechanical stress, apoptosis rate increased significantly and the 
      differentiation speed was slow down. In conclusion, our study revealed the mTOR 
      function on regulating myoblast proliferation, apoptosis and differentiation 
      undergoing mechanical stress.
FAU - Wang, Mengjia
AU  - Wang M
AD  - Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing, 
      Jiangsu Province, P. R. China.
FAU - Mou, Yongbin
AU  - Mou Y
AD  - Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing, 
      Jiangsu Province, P. R. China.
FAU - Da, Yu
AU  - Da Y
AD  - Nanjing Tongren Hospital, School of Medicine, Southeast University Nanjing, 
      Jiangsu Province, P. R. China.
FAU - Yuan, Xiao
AU  - Yuan X
AD  - Qingdao Municipal Hospital Qingdao, Shandong Province, P. R. China.
FAU - Yan, Fuhua
AU  - Yan F
AD  - Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing, 
      Jiangsu Province, P. R. China.
FAU - Lan, Weidong
AU  - Lan W
AD  - Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing, 
      Jiangsu Province, P. R. China.
FAU - Zhang, Fang
AU  - Zhang F
AD  - Nanjing Stomatological Hospital, Medical School of Nanjing University Nanjing, 
      Jiangsu Province, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20181215
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC6325498
OTO - NOTNLM
OT  - Myoblast
OT  - mTOR
OT  - mechanical stress
OT  - proliferation
COIS- None.
EDAT- 2019/01/22 06:00
MHDA- 2019/01/22 06:01
PMCR- 2018/12/15
CRDT- 2019/01/22 06:00
PHST- 2018/07/11 00:00 [received]
PHST- 2018/10/18 00:00 [accepted]
PHST- 2019/01/22 06:00 [entrez]
PHST- 2019/01/22 06:00 [pubmed]
PHST- 2019/01/22 06:01 [medline]
PHST- 2018/12/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2018 Dec 15;10(12):4173-4182. eCollection 2018.