PMID- 30663448
OWN - NLM
STAT- MEDLINE
DCOM- 20200114
LR  - 20200114
IS  - 1532-4311 (Electronic)
IS  - 0882-0139 (Linking)
VI  - 48
IP  - 7
DP  - 2019 Oct
TI  - TNF May Negatively Regulate Phagocytosis of Devil Facial Tumour Disease Cells by 
      Activated Macrophages.
PG  - 691-703
LID - 10.1080/08820139.2018.1515222 [doi]
AB  - Introduction: Macrophage phagocytosis of pathogens and tumour cells is an 
      important early event in protection against infectious disease and cancer. As 
      tumour necrosis factor alpha (TNF) is an important cytokine in macrophage activation, 
      we investigated the involvement of TNF in macrophage phagocytosis of tumour 
      cells. Methods: We used Devil Facial Tumour Disease (DFTD) cancer cells as the 
      target tumour cells. The Tasmanian devil (Sarcophilus harrisii) population is 
      threatened by the transmissible DFTD. Using DFTD cells provided the opportunity 
      to determine if these cells can be phagocytosed and investigate requirement for 
      TNF. As effector cells, bone marrow derived macrophages (BMDMs), generated from 
      C57BL/6 wild type (B6.WT) and C57BL/6 TNF-/- (B6.TNF-/-) mice were used. 
      Phagocytosis of DFTD cells was investigated by confocal microscopy and flow 
      cytometry. Results: DFTD cells were consistently phagocytosed by B6.WT and 
      B6.TNF-/- BMDMs with similar efficiency in vitro. Consequently the DFTD cells are 
      not resistant to phagocytosis. Following activation by exposure to IFNgamma and LPS 
      or LPS alone, B6.TNF-/- BMDMs had higher phagocytic efficiency and lower nitric 
      oxide (NO) production compared to wild-type controls. In addition, NO seems to be 
      unlikely to be the involved in phagocytosis efficiency in IFNgamma and LPS activated 
      B6.TNF-/- macrophages and consequences thereof. Conclusion: Our results indicate 
      that TNF is not required for IFNgamma and LPS or LPS alone activation of macrophage 
      phagocytosis. TNF may negatively regulate macrophage phagocytosis of tumour 
      cells.
FAU - Li, Xinying
AU  - Li X
AD  - Menzies Institute for Medical Research, College of Health and Medicine, 
      University of Tasmania , Hobart , Tasmania , Australia.
AD  - School of Life Science, Anhui Medical University , Hefei , People's Republic of 
      China.
FAU - Darby, Jocelyn
AU  - Darby J
AD  - Menzies Institute for Medical Research, College of Health and Medicine, 
      University of Tasmania , Hobart , Tasmania , Australia.
AD  - School of Medicine, College of Health and Medicine, University of Tasmania , 
      Hobart , Tasmania , Australia.
FAU - Lyons, A Bruce
AU  - Lyons AB
AD  - School of Medicine, College of Health and Medicine, University of Tasmania , 
      Hobart , Tasmania , Australia.
FAU - Woods, Gregory M
AU  - Woods GM
AD  - Menzies Institute for Medical Research, College of Health and Medicine, 
      University of Tasmania , Hobart , Tasmania , Australia.
AD  - School of Medicine, College of Health and Medicine, University of Tasmania , 
      Hobart , Tasmania , Australia.
FAU - Korner, Heinrich
AU  - Korner H
AUID- ORCID: 0000-0003-4610-2814
AD  - Menzies Institute for Medical Research, College of Health and Medicine, 
      University of Tasmania , Hobart , Tasmania , Australia.
AD  - Key Laboratory of Anti-inflammatory and Immunopharmacology, Ministry of 
      Education, Engineering Technology Research Centre of Anti-inflammatory and 
      Immunodrugs in Anhui Province, Institute of Clinical Pharmacology, Anhui Medical 
      University , Hefei, Anhui , People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20190119
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tnf protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Facial Neoplasms/*immunology/pathology/*veterinary
MH  - Interferon-gamma/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/drug effects/*immunology/metabolism
MH  - Marsupialia
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Nitric Oxide/metabolism
MH  - Phagocytosis
MH  - Tumor Necrosis Factor-alpha/deficiency/*immunology
OTO - NOTNLM
OT  - Devil Facial Tumour disease
OT  - Macrophages
OT  - TNF
OT  - nitric oxide
OT  - phagocytosis
EDAT- 2019/01/22 06:00
MHDA- 2020/01/15 06:00
CRDT- 2019/01/22 06:00
PHST- 2019/01/22 06:00 [pubmed]
PHST- 2020/01/15 06:00 [medline]
PHST- 2019/01/22 06:00 [entrez]
AID - 10.1080/08820139.2018.1515222 [doi]
PST - ppublish
SO  - Immunol Invest. 2019 Oct;48(7):691-703. doi: 10.1080/08820139.2018.1515222. Epub 
      2019 Jan 19.