PMID- 30663928
OWN - NLM
STAT- MEDLINE
DCOM- 20191118
LR  - 20201213
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 10
IP  - 4
DP  - 2019
TI  - Bacteroides ovatus ATCC 8483 monotherapy is superior to traditional fecal 
      transplant and multi-strain bacteriotherapy in a murine colitis model.
PG  - 504-520
LID - 10.1080/19490976.2018.1560753 [doi]
AB  - Background and aims: Bacteriotherapy aimed at addressing dysbiosis may be 
      therapeutic for Inflammatory Bowel Diseases (IBDs). We sought to determine if 
      defined Bacteroides-based bacteriotherapy could be an effective and consistent 
      alternative to fecal microbiota transplantation (FMT) in a murine model of IBD. 
      Methods: We induced experimental colitis in 8- 12-week-old C57BL/6 mice using 
      2-3% dextran sodium sulfate. Mice were simultaneously treated by oral gavage with 
      a triple-Bacteroides cocktail, individual Bacteroides strains, FMT using stool 
      from healthy donor mice, or their own stool as a control. Survival, weight loss 
      and markers of inflammation (histology, serum amyloid A, cytokine production) 
      were correlated to 16S rRNA gene profiling of fecal and mucosal microbiomes. 
      Results: Triple-Bacteroides combination therapy was more protective against 
      weight loss and mortality than traditional FMT therapy. B. ovatus ATCC8483 was 
      more effective than any individual strain, or a combination of strains, in 
      preventing weight loss, decreasing histological damage, dampening inflammatory 
      response, and stimulating epithelial recovery. Irrespective of the treatment 
      group, overall Bacteroides abundance associated with treatment success and 
      decreased cytokine production while the presence of Akkermansia correlated with 
      treatment failure. However, the therapeutic benefit associated with high 
      Bacteroides abundance was negated in the presence of Streptococcus. Conclusions: 
      Bacteroides ovatus monotherapy was more consistent and effective than traditional 
      FMT at ameliorating colitis and stimulating epithelial recovery in a murine model 
      of IBD. Given the tolerability of Bacteroides ovatus ATCC 8483 in an active, 
      on-going human study, this therapy may be repurposed for the management of IBD in 
      a clinically expedient timeline.
FAU - Ihekweazu, Faith D
AU  - Ihekweazu FD
AUID- ORCID: 0000-0002-9800-8590
AD  - a Pediatric Gastroenterology, Hepatology and Nutrition , Baylor College of 
      Medicine, Texas Children's Hospital , Houston , TX , USA.
FAU - Fofanova, Tatiana Y
AU  - Fofanova TY
AD  - b Virology and Microbiology , Baylor College of Medicine , Houston , TX , USA.
FAU - Queliza, Karen
AU  - Queliza K
AUID- ORCID: 0000-0002-1165-1269
AD  - a Pediatric Gastroenterology, Hepatology and Nutrition , Baylor College of 
      Medicine, Texas Children's Hospital , Houston , TX , USA.
FAU - Nagy-Szakal, Dorottya
AU  - Nagy-Szakal D
AD  - c Center for Infection and Immunity , Columbia University Mailman School of 
      Public Health , New York , NY , USA.
FAU - Stewart, Christopher J
AU  - Stewart CJ
AUID- ORCID: 0000-0002-6033-338X
AD  - b Virology and Microbiology , Baylor College of Medicine , Houston , TX , USA.
AD  - d Institute of Cellular Medicine , Newcastle University , Newcastle upon Tyne , 
      UK.
FAU - Engevik, Melinda A
AU  - Engevik MA
AUID- ORCID: 0000-0002-9742-9932
AD  - e Pediatric Pathology and Immunology , Baylor College of Medicine , Houston , TX 
      , USA.
FAU - Hulten, Kristina G
AU  - Hulten KG
AUID- ORCID: 0000-0001-7446-157X
AD  - f Pediatric Infectious Disease , Baylor College of Medicine, Texas Children's 
      Hospital , Houston , TX , USA.
FAU - Tatevian, Nina
AU  - Tatevian N
AD  - g Pathology and Laboratory Medicine , The University of Texas Health Science 
      Center at Houston , Houston , TX , USA.
FAU - Graham, David Y
AU  - Graham DY
AD  - h Gastroenterology , Baylor College of Medicine , Houston , TX , USA.
FAU - Versalovic, James
AU  - Versalovic J
AD  - e Pediatric Pathology and Immunology , Baylor College of Medicine , Houston , TX 
      , USA.
FAU - Petrosino, Joseph F
AU  - Petrosino JF
AD  - b Virology and Microbiology , Baylor College of Medicine , Houston , TX , USA.
FAU - Kellermayer, Richard
AU  - Kellermayer R
AD  - a Pediatric Gastroenterology, Hepatology and Nutrition , Baylor College of 
      Medicine, Texas Children's Hospital , Houston , TX , USA.
LA  - eng
GR  - T32 DK007664/DK/NIDDK NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190121
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Animals
MH  - Bacteria/classification/growth & development
MH  - Bacteroides/classification/growth & development/*physiology
MH  - Colitis/chemically induced/pathology/*therapy
MH  - Dextran Sulfate/toxicity
MH  - Disease Models, Animal
MH  - *Fecal Microbiota Transplantation
MH  - Feces/microbiology
MH  - Gastrointestinal Tract/microbiology/pathology
MH  - Inflammation/prevention & control
MH  - Male
MH  - Mice, Inbred C57BL
MH  - RNA, Ribosomal, 16S/genetics
MH  - Survival Analysis
MH  - Treatment Outcome
PMC - PMC6748610
OTO - NOTNLM
OT  - Inflammatory bowel disease
OT  - bacteriotherapy
OT  - dysbiosis
OT  - fecal microbiota transplantation
OT  - microbiota
EDAT- 2019/01/22 06:00
MHDA- 2019/11/19 06:00
PMCR- 2019/01/21
CRDT- 2019/01/22 06:00
PHST- 2019/01/22 06:00 [pubmed]
PHST- 2019/11/19 06:00 [medline]
PHST- 2019/01/22 06:00 [entrez]
PHST- 2019/01/21 00:00 [pmc-release]
AID - 1560753 [pii]
AID - 10.1080/19490976.2018.1560753 [doi]
PST - ppublish
SO  - Gut Microbes. 2019;10(4):504-520. doi: 10.1080/19490976.2018.1560753. Epub 2019 
      Jan 21.