PMID- 30664157
OWN - NLM
STAT- MEDLINE
DCOM- 20190523
LR  - 20200225
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 19
IP  - 3
DP  - 2019 Mar
TI  - MicroRNA‑31 promotes chondrocyte proliferation by targeting C‑X‑C motif chemokine 
      ligand 12.
PG  - 2231-2237
LID - 10.3892/mmr.2019.9859 [doi]
AB  - The present study aimed to investigate the biological function and underlying 
      molecular mechanisms of miR-31 in osteoarthritis (OA). Reverse 
      transcription‑quantitative polymerase chain reaction was used to detect miR‑31 
      expression, and it was found that miR‑31 was downregulated in the cartilage 
      tissues of OA patients. microRNA.org was used to predict the gene targets of 
      miR‑31, and dual luciferase reporter assays were used to verify that C‑X‑C motif 
      chemokine ligand 12 (CXCL12) was a direct target of miR‑31. The human chondrocyte 
      cell line CHON‑001 was used to perform MTT and cell migration assays. Western 
      blotting was used to measure the protein expression of CXCL12, type I collagen 
      and aggrecan. The results suggested that CXCL12 was a target of miR‑31, and the 
      expression of CXCL12 was negatively regulated by miR‑31 in CHON‑001 cells. miR‑31 
      increased CHON‑001 cell viability and migration, as well as the expression of 
      type I collagen and aggrecan. Furthermore, the overexpression of CXCL12 
      eliminated the effects of miR‑31 mimics on CHON‑001 cells. In conclusion, the 
      data indicated that miR‑31 promoted chondrocyte viability and migration by 
      directly targeting CXCL12, which provided evidence for CXCL12 as a potential 
      target in OA therapy.
FAU - Dai, Yankun
AU  - Dai Y
AD  - Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical 
      University and The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223200, 
      P.R. China.
FAU - Liu, Shanglun
AU  - Liu S
AD  - Department of Orthopedics, Huai'an Hospital of Traditional Chinese Medicine, 
      Huai'an, Jiangsu 223200, P.R. China.
FAU - Xie, Xueguan
AU  - Xie X
AD  - Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical 
      University and The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223200, 
      P.R. China.
FAU - Ding, Mingsheng
AU  - Ding M
AD  - Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical 
      University and The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223200, 
      P.R. China.
FAU - Zhou, Quan
AU  - Zhou Q
AD  - Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical 
      University and The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223200, 
      P.R. China.
FAU - Zhou, Xiaoqing
AU  - Zhou X
AD  - Department of Orthopedics, The Affiliated Huai'an Hospital of Xuzhou Medical 
      University and The Second People's Hospital of Huai'an, Huai'an, Jiangsu 223200, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190115
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (CXCL12 protein, human)
RN  - 0 (Chemokine CXCL12)
RN  - 0 (MIRN31 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Aged
MH  - Cartilage/growth & development/metabolism
MH  - Cell Line
MH  - Cell Movement/genetics
MH  - Cell Proliferation/*genetics
MH  - Cell Survival/genetics
MH  - Chemokine CXCL12/*genetics
MH  - Chondrocytes/metabolism/pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Osteoarthritis/*genetics/physiopathology
PMC - PMC6390014
EDAT- 2019/01/22 06:00
MHDA- 2019/05/24 06:00
PMCR- 2019/01/15
CRDT- 2019/01/22 06:00
PHST- 2017/09/27 00:00 [received]
PHST- 2018/12/03 00:00 [accepted]
PHST- 2019/01/22 06:00 [pubmed]
PHST- 2019/05/24 06:00 [medline]
PHST- 2019/01/22 06:00 [entrez]
PHST- 2019/01/15 00:00 [pmc-release]
AID - mmr-19-03-2231 [pii]
AID - 10.3892/mmr.2019.9859 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Mar;19(3):2231-2237. doi: 10.3892/mmr.2019.9859. Epub 2019 Jan 
      15.