PMID- 30664222
OWN - NLM
STAT- MEDLINE
DCOM- 20190524
LR  - 20211204
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 19
IP  - 3
DP  - 2019 Mar
TI  - Mechanical force promotes the proliferation and extracellular matrix synthesis of 
      human gingival fibroblasts cultured on 3D PLGA scaffolds via TGF‑beta expression.
PG  - 2107-2114
LID - 10.3892/mmr.2019.9882 [doi]
AB  - Human gingival fibroblasts (HGFs) are responsible for connective tissue repair 
      and scarring, and are exposed to mechanical forces under physiological and 
      pathological conditions. The exact mechanisms underlying gingival tissue 
      reconstruction under mechanical forces remain unclear. The present study aimfed 
      to investigate the effects of mechanical forces on the proliferation and 
      extracellular matrix synthesis in HGFs by establishing a 3‑dimensional (3D) HGF 
      culture model using poly(lactide‑co‑glycolide) (PLGA) scaffolds. HGFs were 
      cultured in 3D PLGA scaffolds and a mechanical force of 0, 5, 15, 25 or 35 g/cm2 
      was applied to HGFs for 24 h. A mechanical force of 25 g/cm2 induced the highest 
      proliferation rate, and thus was selected for subsequent experiments. Cell 
      viability was determined using the MTT assay at 0, 24, 48 and 72 h. The 
      expression levels of type I collagen (COL‑1) and matrix metallopeptidase (MMP)‑1 
      were examined by reverse transcription‑quantitative polymerase chain reaction and 
      ELISA, and transforming growth factor (TGF)‑beta expression was evaluated by ELISA. 
      The application of mechanical force on HGFs cultured on the 3D PLGA scaffolds 
      resulted in a significant increase in cell proliferation and COL‑1 expression, as 
      well as a decrease in MMP‑1 expression. A TGF‑beta1 inhibitor was also applied, 
      which attenuated the effects of mechanical force on HGF proliferation, and COL‑1 
      and MMP‑1 expression, thus suggesting that TGF‑beta signaling pathways may mediate 
      the mechanical force‑induced alterations observed in HGFs. In conclusion, these 
      findings helped to clarify the mechanisms underlying mechanical force‑induced HGF 
      proliferation and ECM synthesis, which may promote the development of targeted 
      therapeutics to treat various diseases, including gingival atrophy caused by 
      orthodontic treatment.
FAU - Nan, Lan
AU  - Nan L
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Zheng, Yi
AU  - Zheng Y
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Liao, Ni
AU  - Liao N
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Li, Songze
AU  - Li S
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Wang, Yao
AU  - Wang Y
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Chen, Zhixing
AU  - Chen Z
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Wei, Liying
AU  - Wei L
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Zhao, Shuang
AU  - Zhao S
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
FAU - Mo, Shuixue
AU  - Mo S
AD  - Department of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190121
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type I, alpha 1 Chain)
RN  - 0 (TGFB1 protein, human)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - Collagen Type I/*genetics
MH  - Collagen Type I, alpha 1 Chain
MH  - Connective Tissue/growth & development/metabolism
MH  - Extracellular Matrix/*genetics/metabolism
MH  - Fibroblasts/metabolism
MH  - Gene Expression Regulation, Developmental
MH  - Gingiva/growth & development/metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 1/*genetics
MH  - Polylactic Acid-Polyglycolic Acid Copolymer/pharmacology
MH  - Signal Transduction/genetics
MH  - *Stress, Mechanical
MH  - Tissue Scaffolds
MH  - Transforming Growth Factor beta1/antagonists & inhibitors/*genetics
PMC - PMC6390077
EDAT- 2019/01/22 06:00
MHDA- 2019/05/28 06:00
PMCR- 2019/01/21
CRDT- 2019/01/22 06:00
PHST- 2018/01/27 00:00 [received]
PHST- 2018/12/06 00:00 [accepted]
PHST- 2019/01/22 06:00 [pubmed]
PHST- 2019/05/28 06:00 [medline]
PHST- 2019/01/22 06:00 [entrez]
PHST- 2019/01/21 00:00 [pmc-release]
AID - mmr-19-03-2107 [pii]
AID - 10.3892/mmr.2019.9882 [doi]
PST - ppublish
SO  - Mol Med Rep. 2019 Mar;19(3):2107-2114. doi: 10.3892/mmr.2019.9882. Epub 2019 Jan 
      21.