PMID- 30666200
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 9
DP  - 2018
TI  - Correlation Between C-MYC, BCL-2, and BCL-6 Protein Expression and Gene 
      Translocation as Biomarkers in Diagnosis and Prognosis of Diffuse Large B-cell 
      Lymphoma.
PG  - 1497
LID - 10.3389/fphar.2018.01497 [doi]
LID - 1497
AB  - This study investigates the protein expression of C-MYC, BCL-2, and BCL-6 in 
      diffuse large B-cell lymphoma (DLBCL) and their relationship with genetic 
      abnormalities. A retrospective study of 42 cases on paraffin-embedded tissue 
      specimens diagnosed with DLBCL was performed using immunohistochemistry (IHC) and 
      fluorescence in situ hybridization (FISH). The expression of C-MYC, BCL-2, BCL-6 
      protein, and gene abnormalities in these tissue samples was analyzed. The 
      relationship in genetic abnormalities and Ki-67, Hans classification, gender, and 
      age was also evaluated. It was found that the positive rate of C-MYC expression 
      was 47.6% (20/42), the rate of C-MYC gene abnormality was 26.2% (11/42), in which 
      gene translocation accounted for 23.8% (10/42) and gene amplification 2.4% 
      (1/42); C-MYC protein expression was positively correlated with C-MYC gene 
      translocation (chi(2) = 11.813; P = 0.001); C-MYC gene translocation was mainly 
      found in germinal center B cell type (chi(2) = 4.029; P = 0.045). The positive rate 
      of BCL-2 protein expression was 85.71% (36/42), the positive rate of 
      translocation was 42.86% (18/42) and the amplification rate was 26.19% (11/42); 
      the overexpression of BCL-2 protein was correlated with the BCL-2 translocation 
      (chi(2) = 3.407; P = 0.029). The positive rate of BCL-6 protein expression was 
      45.24% (19/42), the positive rate of BCL-6 translocation was 14.29% (6/42) and 
      the positive rate of BCL-6 amplification was 7.14% (3/42); the overexpression of 
      BCL-6 protein was significantly correlated with BCL-6 translocation (chi(2) = 
      6.091; P = 0.014). The Ki-67 index was significantly higher in C-MYC 
      translocation cases than in non-C-MYC translocation cases (chi2 = 4.492; P = 
      0.034). Taken together, our results suggest that the protein expression of C-MYC, 
      BCL-2, and BCL-6 are positively correlated with their gene translocation. 
      Overexpression of C-MYC, BCL-2, BCL-6 protein suggests the possibility of 
      translocation. Therefore, immunohistochemical detection of C-MYC, BCL-2, and 
      BCL-6 are useful in diagnosis and prognosis of DLBCL.
FAU - Zhang, YunXiang
AU  - Zhang Y
AD  - Department of Pathology, Weifang People's Hospital, Weifang, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Pathology, Weifang People's Hospital, Weifang, China.
FAU - Ren, Cuiai
AU  - Ren C
AD  - Department of Pathology, Weifang People's Hospital, Weifang, China.
FAU - Yu, Hai
AU  - Yu H
AD  - Department of Pathology, Werfang Traditional Chinese Hospital, Weifang, China.
FAU - Fang, Wenjia
AU  - Fang W
AD  - Department of Clinical Medicine, Nanchang University Medical College, Nanchang, 
      China.
FAU - Zhang, Na
AU  - Zhang N
AD  - Department of Pathology, Weifang People's Hospital, Weifang, China.
FAU - Gao, Sumei
AU  - Gao S
AD  - Department of Pathology, Weifang People's Hospital, Weifang, China.
FAU - Hou, Qian
AU  - Hou Q
AD  - Department of Pathology, Weifang People's Hospital, Weifang, China.
LA  - eng
PT  - Journal Article
DEP - 20190107
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC6330311
OTO - NOTNLM
OT  - BCL-2
OT  - BCL-6
OT  - C-MYC
OT  - diffuse lange B-cell lymphoma
OT  - gene amplification
OT  - gene translocation
EDAT- 2019/01/23 06:00
MHDA- 2019/01/23 06:01
PMCR- 2019/01/07
CRDT- 2019/01/23 06:00
PHST- 2018/11/05 00:00 [received]
PHST- 2018/12/07 00:00 [accepted]
PHST- 2019/01/23 06:00 [entrez]
PHST- 2019/01/23 06:00 [pubmed]
PHST- 2019/01/23 06:01 [medline]
PHST- 2019/01/07 00:00 [pmc-release]
AID - 10.3389/fphar.2018.01497 [doi]
PST - epublish
SO  - Front Pharmacol. 2019 Jan 7;9:1497. doi: 10.3389/fphar.2018.01497. eCollection 
      2018.