PMID- 30669263
OWN - NLM
STAT- MEDLINE
DCOM- 20191127
LR  - 20200309
IS  - 2073-4409 (Print)
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 8
IP  - 1
DP  - 2019 Jan 18
TI  - Retinal Neuron Is More Sensitive to Blue Light-Induced Damage than Glia Cell Due 
      to DNA Double-Strand Breaks.
LID - 10.3390/cells8010068 [doi]
LID - 68
AB  - Blue light is a major component of visible light and digital displays. 
      Over-exposure to blue light could cause retinal damage. However, the mechanism of 
      its damage is not well defined. Here, we demonstrate that blue light (900 lux) 
      impairs cell viability and induces cell apoptosis in retinal neurocytes in vitro. 
      A DNA electrophoresis assay shows severe DNA damage in retinal neurocytes at 2 h 
      after blue light treatment. gamma-H2AX foci, a specific marker of DNA double-strand 
      breaks (DSBs), is mainly located in the Map2-posotive neuron other than the glia 
      cell. After assaying the expression level of proteins related to DNA repair, 
      Mre11, Ligase IV and Ku80, we find that Ku80 is up-regulated in retinal 
      neurocytes after blue light treatment. Interestingly, Ku80 is mainly expressed in 
      glia fibrillary acidic protein (GFAP)-positive glia cells. Moreover, following 
      blue light exposure in vivo, DNA DSBs are shown in the ganglion cell layer and 
      only observed in Map2-positive cells. Furthermore, long-term blue light exposure 
      significantly thinned the retina in vivo. Our findings demonstrate that blue 
      light induces DNA DSBs in retinal neurons, and the damage is more pronounced 
      compared to glia cells. Thus, this study provides new insights into the 
      mechanisms of the effect of blue light on the retina.
FAU - Chen, Pei
AU  - Chen P
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. peichen912@163.com.
FAU - Lai, Zhipeng
AU  - Lai Z
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. LaiZhp3@mail2.sysu.edu.cn.
FAU - Wu, Yihui
AU  - Wu Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. wuyh45@mail2.sysu.edu.cn.
FAU - Xu, Lijun
AU  - Xu L
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. xlj727@163.com.
FAU - Cai, Xiaoxiao
AU  - Cai X
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. caixx1987@126.com.
FAU - Qiu, Jin
AU  - Qiu J
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. qiujin19940916@163.com.
FAU - Yang, Panyang
AU  - Yang P
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. yangpanyang9@sina.com.
FAU - Yang, Meng
AU  - Yang M
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. 18819253882@163.com.
FAU - Zhou, Pan
AU  - Zhou P
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. zhoup23@mail2.sysu.edu.cn.
FAU - Zhuang, Jiejie
AU  - Zhuang J
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. zhuangjiejie@aliyun.com.
FAU - Ge, Jian
AU  - Ge J
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. gejian@mail.sysu.edu.cn.
FAU - Yu, Keming
AU  - Yu K
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. yukeming@mail.sysu.edu.cn.
FAU - Zhuang, Jing
AU  - Zhuang J
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen 
      University, Guangzhou 510060, China. zhuangj@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190118
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - EC 4.2.99.- (Ku Autoantigen)
SB  - IM
MH  - Animals
MH  - Apoptosis/radiation effects
MH  - Cell Survival/radiation effects
MH  - DNA Breaks, Double-Stranded/*radiation effects
MH  - Ku Autoantigen/metabolism
MH  - *Light
MH  - Neuroglia/*pathology/*radiation effects
MH  - Rats, Sprague-Dawley
MH  - Retinal Neurons/*pathology/*radiation effects
MH  - Up-Regulation/genetics/radiation effects
PMC - PMC6356720
OTO - NOTNLM
OT  - DNA double strand breaks
OT  - blue light
OT  - glia cell
OT  - retinal neuron
COIS- The authors declare no conflicts of interest.
EDAT- 2019/01/24 06:00
MHDA- 2019/01/24 06:01
PMCR- 2019/01/01
CRDT- 2019/01/24 06:00
PHST- 2018/12/01 00:00 [received]
PHST- 2019/01/06 00:00 [revised]
PHST- 2019/01/15 00:00 [accepted]
PHST- 2019/01/24 06:00 [entrez]
PHST- 2019/01/24 06:00 [pubmed]
PHST- 2019/01/24 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - cells8010068 [pii]
AID - cells-08-00068 [pii]
AID - 10.3390/cells8010068 [doi]
PST - epublish
SO  - Cells. 2019 Jan 18;8(1):68. doi: 10.3390/cells8010068.