PMID- 30670774
OWN - NLM
STAT- MEDLINE
DCOM- 20200616
LR  - 20220419
IS  - 1530-0447 (Electronic)
IS  - 0031-3998 (Linking)
VI  - 85
IP  - 4
DP  - 2019 Mar
TI  - Passive smoking induces pediatric asthma by affecting the balance of Treg/Th17 
      cells.
PG  - 469-476
LID - 10.1038/s41390-019-0276-0 [doi]
AB  - BACKGROUND: We aimed to explore the effects of passive smoking on the severity of 
      pediatric asthma and associated molecular mechanisms. METHODS: A total of 378 
      children with asthma were assigned into four groups according to asthma severity 
      (from grades I to IV). Univariate and multivariate regression analyses were used 
      to analyze possible factors associated with asthma severity in children. 
      Environmental tobacco smoke (ETS) exposure was measured via cotinine 
      concentration in urine. Serum levels of immunoglobulin E (IgE) and cytokines were 
      measured using allergen diagnostic and ELISA (enzyme-linked immunosorbent assay) 
      kits. The percentage of T-regulatory (Treg) and T-helper type 17 (Th17) cells in 
      peripheral blood mononuclear cells (PMBCs) were measured by flow cytometry. Treg- 
      and Th17-associated transcription factors from PMBCs were measured by using ELISA 
      kits. RESULTS: The levels of ETS and serum IgE, and the duration and amounts of 
      passive smoking were closely associated with asthma severity. Passive smoking 
      significantly reduced the levels of FoxP3 (Forkhead/winged helix transcription 
      factor) and tumor growth factor-beta, which were associated with Treg cells, and 
      increased the levels of interleukin-17A and interleukin-23, which were associated 
      with Th17 cells. Meanwhile, passive smoking significantly reduced the ratio of 
      Treg/Th17 cells (P < 0.05). CONCLUSIONS: Passive smoking was closely associated 
      with the severity of childhood asthma by affecting the balance of Treg/Th17 
      cells.
FAU - Jing, Wei
AU  - Jing W
AD  - Department of Pediatrics, The Affiliated Hospital of Changchun University of 
      Traditional Chinese Medicine, 130021, Changchun, China. jingwei20166@126.com.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Pediatrics, The Affiliated Hospital of Changchun University of 
      Traditional Chinese Medicine, 130021, Changchun, China.
FAU - Liu, Qingbin
AU  - Liu Q
AD  - Department of Pediatrics, The Affiliated Hospital of Changchun University of 
      Traditional Chinese Medicine, 130021, Changchun, China.
LA  - eng
PT  - Journal Article
DEP - 20190116
PL  - United States
TA  - Pediatr Res
JT  - Pediatric research
JID - 0100714
RN  - 0 (Cytokines)
RN  - 0 (Tobacco Smoke Pollution)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Air Pollution
MH  - Asthma/*immunology/physiopathology
MH  - Child
MH  - Child, Preschool
MH  - Cytokines/blood
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Respiratory Hypersensitivity
MH  - Severity of Illness Index
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Th17 Cells/*immunology
MH  - *Tobacco Smoke Pollution
EDAT- 2019/01/24 06:00
MHDA- 2020/06/17 06:00
CRDT- 2019/01/24 06:00
PHST- 2018/05/15 00:00 [received]
PHST- 2018/12/09 00:00 [accepted]
PHST- 2018/11/04 00:00 [revised]
PHST- 2019/01/24 06:00 [pubmed]
PHST- 2020/06/17 06:00 [medline]
PHST- 2019/01/24 06:00 [entrez]
AID - 10.1038/s41390-019-0276-0 [pii]
AID - 10.1038/s41390-019-0276-0 [doi]
PST - ppublish
SO  - Pediatr Res. 2019 Mar;85(4):469-476. doi: 10.1038/s41390-019-0276-0. Epub 2019 
      Jan 16.