PMID- 30672259
OWN - NLM
STAT- MEDLINE
DCOM- 20190730
LR  - 20190730
IS  - 0255-2930 (Print)
IS  - 0255-2930 (Linking)
VI  - 39
IP  - 1
DP  - 2019 Jan 12
TI  - [Effects of huayu tongluo moxibustion on learning and memory ability and the 
      hippocampal BDNF/TrkB expressions in the rats of vascular dementia].
PG  - 65-71
LID - 10.13703/j.0255-2930.2019.01.014 [doi]
AB  - OBJECTIVE: To explore the effects of huayu tongluo (resolving stasis, promoting 
      collateral circulation) moxibustion on learning and memory ability and the 
      expressions of brain derived neurotrophic factor (BDNF) and tyrosine kinase B 
      (TrkB) in the rats of vascular dementia (VD) in the microenvironment of 
      neurovascular niche. METHODS: Using 2-vessel occlusion (2-VO), the VD rat models 
      were duplicated. The neural stem cells (NSCs) labeled with lentiviral 
      vector-mediated enhanced green fluorescent protein (EGFP) were co-cultured with 
      endothelial progenitor cells (EPCs) to structure the NSCs + EPCs implant. The 
      implant was transplanted into the lateral ventricle of VD rats and the VD rat 
      models with neurovascular niche were established. In No.1 experiment, the 
      successful-modeled rats were divided into 3 groups, i.e. a NSCs + EPCs 
      moxibustion group, a NSCs + EPCs blank group and a model group, 12 rats in each 
      one. No any treatment was provided in the model group and the NSCs + EPCs blank 
      group. The huayu tongluo moxibustion therapy was adopted in the NSCs + EPCs 
      moxibustion group, in which, the suspending moxibustion technique was applied to 
      "Baihui" (GV 20), "Dazhui" (GV 14) and "Shenting" (GV 24), 20 min at each 
      acupoint. The treatment was given once every day and a 14-day treatment was as 
      one course. Totally, 3 courses of treatment were required. At the end of 
      treatment, Morris water maze experiment was adopted to determine the learning and 
      memory ability of the rats in each group. In the No.2 experiment, the model rats 
      were divided into 3 groups, a NSCs + EPCs moxibustion group, a NSCs + EPCs blank 
      group and a model group, 18 rats in each one. In each group, according to the 
      durations of treatment, 3 subgroups were divided and 6 rats in each one. The 
      intervention method was same as the No.1 experiment. Additionally, after 
      corresponding treatment course, using perfusion, the brains were collected in 
      each subgroup and the slices were frozen. BDNF/TrkB expressions were observed in 
      the immunofluorescence test. RESULTS: After treatment, in the NSCs + EPCs 
      moxibustion group, the escape incubation was reduced, the time of the first 
      running-cross platform was shortened and the frequency of running-cross platform 
      increased as compared with the model group and the NSCs + EPCs blank group 
      (P<0.01, P<0.05). The protein expressions were increased in tendency among the 3 
      courses of treatment in the NSCs + EPCs moxibustion group, indicating the 
      significant differences (all P<0.05), in which, the increase of the protein 
      expressions in the NSCs + EPCs moxibustion group was better than the NSCs + EPCs 
      blank group (P<0.05, P<0.01). CONCLUSION: The huayu tongluo moxibustion therapy 
      is the effective approach to VD in clinical treatment. This therapy up-regulates 
      the BDNF/TrkB protein expressions in the microenvironment of neurovascular niche, 
      co-modulates NSCs-EPCs coupling mechanism, promotes nerve neogenesis and repairs 
      the injured nerve.
FAU - Zhang, Yuan-Yuan
AU  - Zhang YY
AD  - the First Affiliated Hospital of Anhui University of TCM, Hefei 230031, China.
FAU - Zhang, Xiao-Jun
AU  - Zhang XJ
AD  - Anhui Integrated Chinese and Western Medicine Hospital.
FAU - Yang, Jun
AU  - Yang J
AD  - the First Affiliated Hospital of Anhui University of TCM, Hefei 230031, China.
FAU - Fan, Yin-Qiu
AU  - Fan YQ
AD  - Graduate School of Anhui University of TCM.
FAU - Cui, Jing-Cheng
AU  - Cui JC
AD  - Graduate School of Anhui University of TCM.
FAU - Wang, Pin
AU  - Wang P
AD  - School of Acupuncture, Moxibustion and Tuina, Anhui University of TCM, Hefei 
      230031.
FAU - Zhang, Qing-Ping
AU  - Zhang QP
AD  - School of Acupuncture, Moxibustion and Tuina, Anhui University of TCM, Hefei 
      230031.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhen Jiu
JT  - Zhongguo zhen jiu = Chinese acupuncture & moxibustion
JID - 8600658
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (tongluo)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 3.4.21.47 (Complement Factor B)
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Complement Factor B
MH  - *Dementia, Vascular/metabolism
MH  - Drugs, Chinese Herbal
MH  - Hippocampus
MH  - *Moxibustion
MH  - Protein-Tyrosine Kinases/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - BDNF
OT  - TrkB
OT  - huayu tongluo moxibustion therapy
OT  - neurovascular niche
OT  - vascular dementia
EDAT- 2019/01/24 06:00
MHDA- 2019/07/31 06:00
CRDT- 2019/01/24 06:00
PHST- 2019/01/24 06:00 [entrez]
PHST- 2019/01/24 06:00 [pubmed]
PHST- 2019/07/31 06:00 [medline]
AID - 10.13703/j.0255-2930.2019.01.014 [doi]
PST - ppublish
SO  - Zhongguo Zhen Jiu. 2019 Jan 12;39(1):65-71. doi: 
      10.13703/j.0255-2930.2019.01.014.