PMID- 30673440
OWN - NLM
STAT- MEDLINE
DCOM- 20190225
LR  - 20190225
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 102
IP  - 6
DP  - 2018 Jun
TI  - Two Novel Fungal Symbionts Fusarium kuroshium sp. nov. and Graphium kuroshium sp. 
      nov. of Kuroshio Shot Hole Borer (Euwallacea sp. nr. fornicatus) Cause Fusarium 
      Dieback on Woody Host Species in California.
PG  - 1154-1164
LID - 10.1094/PDIS-07-17-1042-RE [doi]
AB  - Shot hole borer (SHB)-Fusarium dieback (FD) is a new pest-disease complex 
      affecting numerous tree species in California and is vectored by two distinct, 
      but related ambrosia beetles (Euwallacea sp. nr. fornicatus) called polyphagous 
      shot hole borer (PSHB) and Kuroshio shot hole borer (KSHB). These pest-disease 
      complexes cause branch dieback and tree mortality on numerous wildland and 
      landscape tree species, as well as agricultural tree species, primarily avocado. 
      The recent discovery of KSHB in California initiated an investigation of fungal 
      symbionts associated with the KSHB vector. Ten isolates of Fusarium sp. and 
      Graphium sp., respectively, were recovered from the mycangia of adult KSHB 
      females captured in three different locations within San Diego County and 
      compared with the known symbiotic fungi of PSHB. Multigene phylogenetic analyses 
      of the internal transcribed spacer region (ITS), translation elongation factor-1 
      alpha (TEF1-alpha), and RNA polymerase II subunit (RPB1, RPB2) regions as well as 
      morphological comparisons revealed that two novel fungal associates Fusarium 
      kuroshium sp. nov. and Graphium kuroshium sp. nov. obtained from KSHB were 
      related to, but distinct from the fungal symbionts F. euwallaceae and G. 
      euwallaceae associated with PSHB in California. Pathogenicity tests on healthy, 
      young avocado plants revealed F. kuroshium and G. kuroshium to be pathogenic. 
      Lesion lengths from inoculation of F. kuroshium were found to be significantly 
      shorter compared with those caused by F. euwallaceae, while no difference in 
      symptom severity was detected between Graphium spp. associated with KSHB and 
      PSHB. These findings highlight the pest disease complexes of KSHB-FD and PSHB-FD 
      as distinct, but collective threats adversely impacting woody hosts throughout 
      California.
FAU - Na, Francis
AU  - Na F
AD  - Department of Plant Pathology and Microbiology, University of California, 
      Riverside, 92521.
FAU - Carrillo, Joseph D
AU  - Carrillo JD
AD  - Department of Plant Pathology and Microbiology, University of California, 
      Riverside, 92521.
FAU - Mayorquin, Joey S
AU  - Mayorquin JS
AD  - Department of Plant Pathology and Microbiology, University of California, 
      Riverside, 92521.
FAU - Ndinga-Muniania, Cedric
AU  - Ndinga-Muniania C
AD  - Department of Plant Pathology and Microbiology, University of California, 
      Riverside, 92521.
FAU - Stajich, Jason E
AU  - Stajich JE
AD  - Department of Plant Pathology and Microbiology, University of California, 
      Riverside, 92521.
FAU - Stouthamer, Richard
AU  - Stouthamer R
AD  - Department of Entomology, University of California, Riverside, 92521.
FAU - Huang, Yin-Tse
AU  - Huang YT
AD  - Department of Plant Pathology, National Chung Hsing University, Taichung 402, 
      Taiwan, ROC, and School of Forest Resources and Conservation, Institute of Food 
      and Agricultural Sciences, University of Florida, Gainesville.
FAU - Lin, Yu-Ting
AU  - Lin YT
AD  - Department of Plant Pathology, National Chung Hsing University, Taichung 402, 
      Taiwan, ROC.
FAU - Chen, Chi-Yu
AU  - Chen CY
AD  - Department of Plant Pathology, National Chung Hsing University, Taichung 402, 
      Taiwan, ROC.
FAU - Eskalen, Akif
AU  - Eskalen A
AD  - Department of Plant Pathology and Microbiology, University of California, 
      Riverside, 92521.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20180402
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - Animals
MH  - Ascomycota/*genetics/physiology
MH  - California
MH  - Coleoptera/*microbiology/physiology
MH  - DNA, Fungal/genetics/isolation & purification
MH  - Fusarium/*genetics/physiology
MH  - Persea/microbiology
MH  - Phylogeny
MH  - Plant Diseases/*microbiology
MH  - *Symbiosis
EDAT- 2019/01/24 06:00
MHDA- 2019/02/26 06:00
CRDT- 2019/01/24 06:00
PHST- 2019/01/24 06:00 [entrez]
PHST- 2019/01/24 06:00 [pubmed]
PHST- 2019/02/26 06:00 [medline]
AID - 10.1094/PDIS-07-17-1042-RE [doi]
PST - ppublish
SO  - Plant Dis. 2018 Jun;102(6):1154-1164. doi: 10.1094/PDIS-07-17-1042-RE. Epub 2018 
      Apr 2.