PMID- 30676001
OWN - NLM
STAT- MEDLINE
DCOM- 20200408
LR  - 20200408
IS  - 2157-6564 (Print)
IS  - 2157-6580 (Electronic)
IS  - 2157-6564 (Linking)
VI  - 8
IP  - 2
DP  - 2019 Feb
TI  - Challenges Toward the Identification of Predictive Markers for Human Mesenchymal 
      Stromal Cells Chondrogenic Potential.
PG  - 194-204
LID - 10.1002/sctm.18-0147 [doi]
AB  - Human bone marrow derived mesenchymal stromal cells (BMSCs) represent a putative 
      cell source candidate for tissue engineering-based strategies to repair cartilage 
      and bone. However, traditional isolation of BMSCs by adhesion to plastic leads to 
      very heterogeneous cell populations, accounting for high variability of 
      chondrogenic differentiation outcome, both across donors and across clonally 
      derived strains. Identification of putative surface markers able to select BMSC 
      subpopulations with higher chondrogenic capacity (CC) and reduced variance in 
      chondrogenic differentiation could aid the development of BMSC-based cartilage 
      and bone regeneration approaches. With the goal to identify predictive markers 
      for chondrogenic BMSC populations, we assessed the gene expression profile of 
      single cell-derived clones with high and low CC. While a clustering between high 
      and low CC clones was observed for one donor, donor-to-donor variability hampered 
      the possibility to achieve conclusive results when different donors were 
      considered. Nevertheless, increased NCAM1/CD56 expression correlated in clones 
      derived from one donor with higher CC, the same trend was observed for three 
      additional donors (even if no significance was achieved). Enriching multiclonal 
      BMSCs for CD56(+) expression led to an increase in CC, though still highly 
      affected by donor-to-donor variability. Our study finally suggests that 
      definition of predictive marker(s) for BMSCs chondrogenesis is challenged by the 
      large donor heterogeneity of these cells, and by the high complexity and 
      plasticity of the BMSCs system. Multiple pathways and external parameters may be 
      indeed involved in determining the chondrogenic potential of BMSCs, making the 
      identification of putative markers still an open issue. Stem Cells Translational 
      Medicine 2019;8:194&11.
CI  - (c) 2019 The Authors. Stem Cells Translational Medicine published by Wiley 
      Periodicals, Inc. on behalf of AlphaMed Press.
FAU - Studle, Chiara
AU  - Studle C
AD  - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 
      Switzerland.
FAU - Occhetta, Paola
AU  - Occhetta P
AD  - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 
      Switzerland.
FAU - Geier, Florian
AU  - Geier F
AD  - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 
      Switzerland.
FAU - Mehrkens, Arne
AU  - Mehrkens A
AD  - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 
      Switzerland.
FAU - Barbero, Andrea
AU  - Barbero A
AD  - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 
      Switzerland.
FAU - Martin, Ivan
AU  - Martin I
AUID- ORCID: 0000-0001-6493-0432
AD  - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, 
      Switzerland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Stem Cells Transl Med
JT  - Stem cells translational medicine
JID - 101578022
RN  - 0 (Biomarkers)
SB  - IM
MH  - Adult
MH  - Biomarkers/*metabolism
MH  - Bone Marrow/metabolism
MH  - Bone Marrow Cells/metabolism
MH  - Cartilage/metabolism
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Chondrocytes/metabolism
MH  - Chondrogenesis/*physiology
MH  - Female
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Stromal Cells/*metabolism
MH  - Tissue Engineering/methods
PMC - PMC6344903
OTO - NOTNLM
OT  - Chondrogenesis
OT  - Human bone marrow derived mesenchymal stromal cells
OT  - NCAM1/CD56
OT  - RNA sequencing
OT  - Selection markers
OT  - Tissue engineering
EDAT- 2019/01/25 06:00
MHDA- 2020/04/09 06:00
PMCR- 2019/01/24
CRDT- 2019/01/25 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2018/09/05 00:00 [revised]
PHST- 2018/09/21 00:00 [accepted]
PHST- 2019/01/25 06:00 [entrez]
PHST- 2019/01/25 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2019/01/24 00:00 [pmc-release]
AID - SCT312424 [pii]
AID - 10.1002/sctm.18-0147 [doi]
PST - ppublish
SO  - Stem Cells Transl Med. 2019 Feb;8(2):194-204. doi: 10.1002/sctm.18-0147.