PMID- 30678142
OWN - NLM
STAT- MEDLINE
DCOM- 20190513
LR  - 20231006
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 3
DP  - 2019 Jan 22
TI  - Cold-Responsive Regions of Paradigm Cold-Shock and Non-Cold-Shock mRNAs 
      Responsible for Cold Shock Translational Bias.
LID - 10.3390/ijms20030457 [doi]
LID - 457
AB  - In Escherichia coli, the mRNA transcribed from the main cold-shock gene cspA is a 
      thermosensor, which at low temperature adopts a conformation particularly 
      suitable for translation in the cold. Unlike cspA, its paralogue cspD is 
      expressed only at 37  degrees C, is toxic so cannot be hyper-expressed in E. coli and is 
      poorly translated in vitro, especially at low temperature. In this work, chimeric 
      mRNAs consisting of different segments of cspA and cspD were constructed to 
      determine if parts of cspA could confer cold-responsive properties to cspD to 
      improve its expression. The activities of these chimeric mRNAs in translation and 
      in partial steps of translation initiation such as formation of 30S initiation 
      complexes and 50S subunits docking to 30S complexes to yield 70S initiation 
      complexes were analyzed. We show that the 5' untranslated region (5'UTR) of cspA 
      mRNA is sufficient to improve the translation of cspD mRNA at 37  degrees C whereas both 
      the 5'UTR and the region immediately downstream the cspA mRNA initiation triplet 
      are essential for translation at low temperature. Furthermore, the translational 
      apparatus of cold-stressed cells contains trans-active elements targeting both 
      5'UTR and downstream regions of cspA mRNA, thereby improving translation of 
      specific chimeric constructs at both 15 and 37  degrees C.
FAU - Giuliodori, Anna Maria
AU  - Giuliodori AM
AD  - Laboratory of Genetics, University of Camerino, 62032 Camerino, Italy. 
      annamaria.giuliodori@unicam.it.
FAU - Fabbretti, Attilio
AU  - Fabbretti A
AD  - Laboratory of Genetics, University of Camerino, 62032 Camerino, Italy. 
      attilio.fabbretti@unicam.it.
FAU - Gualerzi, Claudio
AU  - Gualerzi C
AD  - Laboratory of Genetics, University of Camerino, 62032 Camerino, Italy. 
      claudio.gualerzi@unicam.it.
LA  - eng
GR  - RBFR130VS5 001/MIUR/
PT  - Journal Article
DEP - 20190122
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Bacterial Proteins)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Bacterial Proteins/*genetics/metabolism
MH  - Cold-Shock Response/genetics/*physiology
MH  - Escherichia coli/genetics/physiology
MH  - Gene Expression Regulation, Bacterial/genetics/physiology
MH  - Protein Biosynthesis/genetics/physiology
MH  - RNA, Messenger/*metabolism
PMC - PMC6386945
OTO - NOTNLM
OT  - chimeric mRNAs
OT  - cold acclimation
OT  - protein synthesis
OT  - translation initiation
OT  - translational bias
COIS- The authors declare no conflict of interest.
EDAT- 2019/01/27 06:00
MHDA- 2019/05/14 06:00
PMCR- 2019/02/01
CRDT- 2019/01/26 06:00
PHST- 2018/12/04 00:00 [received]
PHST- 2019/01/12 00:00 [revised]
PHST- 2019/01/16 00:00 [accepted]
PHST- 2019/01/26 06:00 [entrez]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/05/14 06:00 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - ijms20030457 [pii]
AID - ijms-20-00457 [pii]
AID - 10.3390/ijms20030457 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 Jan 22;20(3):457. doi: 10.3390/ijms20030457.