PMID- 30679557
OWN - NLM
STAT- MEDLINE
DCOM- 20200810
LR  - 20211204
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Jan 24
TI  - Marine biogenics in sea spray aerosols interact with the mTOR signaling pathway.
PG  - 675
LID - 10.1038/s41598-018-36866-3 [doi]
LID - 675
AB  - Sea spray aerosols (SSAs) have profound effects on our climate and ecosystems. 
      They also contain microbiota and biogenic molecules which could affect human 
      health. Yet the exposure and effects of SSAs on human health remain poorly 
      studied. Here, we exposed human lung cancer cells to extracts of a natural sea 
      spray aerosol collected at the seashore in Belgium, a laboratory-generated SSA, 
      the marine algal toxin homoyessotoxin and a chemical inhibitor of the mammalian 
      target of rapamycin (mTOR) pathway. We observed significant increased expression 
      of genes related to the mTOR pathway and Proprotein convertase subtilisin/kexin 
      type 9 (PCSK9) after exposure to homoyessotoxin and the laboratory-generated SSA. 
      In contrast, we observed a significant decrease in gene expression in the mTOR 
      pathway and of PCSK9 after exposure to the natural SSA and the mTOR inhibitor, 
      suggesting induction of apoptosis. Our results indicate that marine biogenics in 
      SSAs interact with PCSK9 and the mTOR pathway and can be used in new potential 
      pharmaceutical applications. Overall, our results provide a substantial molecular 
      evidence base for potential beneficial health effects at environmentally relevant 
      concentrations of natural SSAs.
FAU - Asselman, Jana
AU  - Asselman J
AUID- ORCID: 0000-0003-0185-6516
AD  - Laboratory of Environmental Toxicology and Aquatic Ecology, Environmental 
      Toxicology Unit - GhEnToxLab, Ghent University, Campus Coupure, Coupure Links 
      653, Building F - 2nd Floor, Ghent, Belgium. Jana.Asselman@UGent.be.
FAU - Van Acker, Emmanuel
AU  - Van Acker E
AD  - Laboratory of Environmental Toxicology and Aquatic Ecology, Environmental 
      Toxicology Unit - GhEnToxLab, Ghent University, Campus Coupure, Coupure Links 
      653, Building F - 2nd Floor, Ghent, Belgium. Emmanuel.VanAcker@UGent.be.
FAU - De Rijcke, Maarten
AU  - De Rijcke M
AD  - Flanders Marine Institute (VLIZ), InnovOcean site, Wandelaarkaai 7, 8400, Ostend, 
      Belgium.
FAU - Tilleman, Laurentijn
AU  - Tilleman L
AUID- ORCID: 0000-0001-7584-1179
AD  - Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, 
      Ghent University, Campus UZ, Ottergemse Steenweg 460, 9000, Ghent, Belgium.
FAU - Van Nieuwerburgh, Filip
AU  - Van Nieuwerburgh F
AUID- ORCID: 0000-0001-8815-5485
AD  - Laboratory for Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, 
      Ghent University, Campus UZ, Ottergemse Steenweg 460, 9000, Ghent, Belgium.
FAU - Mees, Jan
AU  - Mees J
AD  - Flanders Marine Institute (VLIZ), InnovOcean site, Wandelaarkaai 7, 8400, Ostend, 
      Belgium.
FAU - De Schamphelaere, Karel A C
AU  - De Schamphelaere KAC
AD  - Laboratory of Environmental Toxicology and Aquatic Ecology, Environmental 
      Toxicology Unit - GhEnToxLab, Ghent University, Campus Coupure, Coupure Links 
      653, Building F - 2nd Floor, Ghent, Belgium.
FAU - Janssen, Colin R
AU  - Janssen CR
AD  - Laboratory of Environmental Toxicology and Aquatic Ecology, Environmental 
      Toxicology Unit - GhEnToxLab, Ghent University, Campus Coupure, Coupure Links 
      653, Building F - 2nd Floor, Ghent, Belgium.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190124
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (41a-homoyessotoxin)
RN  - 0 (Aerosols)
RN  - 0 (Oxocins)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 3.4.21.- (PCSK9 protein, human)
RN  - EC 3.4.21.- (Proprotein Convertase 9)
SB  - IM
MH  - A549 Cells
MH  - Aerosols/*chemistry/*pharmacology
MH  - Belgium
MH  - Dinoflagellida/chemistry
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Oceans and Seas
MH  - Oxocins/*pharmacology
MH  - Proprotein Convertase 9/genetics
MH  - Seawater/chemistry
MH  - TOR Serine-Threonine Kinases/*metabolism
PMC - PMC6345880
COIS- The authors declare no competing interests.
EDAT- 2019/01/27 06:00
MHDA- 2020/08/11 06:00
PMCR- 2019/01/24
CRDT- 2019/01/26 06:00
PHST- 2018/06/19 00:00 [received]
PHST- 2018/11/25 00:00 [accepted]
PHST- 2019/01/26 06:00 [entrez]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2020/08/11 06:00 [medline]
PHST- 2019/01/24 00:00 [pmc-release]
AID - 10.1038/s41598-018-36866-3 [pii]
AID - 36866 [pii]
AID - 10.1038/s41598-018-36866-3 [doi]
PST - epublish
SO  - Sci Rep. 2019 Jan 24;9(1):675. doi: 10.1038/s41598-018-36866-3.