PMID- 30679723
OWN - NLM
STAT- MEDLINE
DCOM- 20200803
LR  - 20200803
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Jan 24
TI  - Spectral photoacoustic imaging to estimate in vivo placental oxygenation during 
      preeclampsia.
PG  - 558
LID - 10.1038/s41598-018-37310-2 [doi]
LID - 558
AB  - Preeclampsia is a pregnancy-related hypertensive disorder accounting for 14% of 
      global maternal deaths annually. Preeclampsia - maternal hypertension and 
      proteinuria - is promoted by placental ischemia resulting from reduced 
      uteroplacental perfusion. Here, we assess longitudinal changes in placental 
      oxygenation during preeclampsia using spectral photoacoustic imaging. Spectral 
      photoacoustic images were acquired of the placenta of normal pregnant (NP) and 
      preeclamptic reduced uterine perfusion pressure (RUPP) Sprague Dawley rats on 
      gestational days (GD) 14, 16, and 18, corresponding to mid- to late gestation 
      (n = 10 per cohort). Two days after implementation of the RUPP surgical model, 
      placental oxygen saturation decreased 12% in comparison with NP. Proteinuria was 
      determined from a 24-hour urine collection prior to imaging on GD18. Blood 
      pressure measurements were obtained on GD18 after imaging. Placental hypoxia in 
      the RUPP was confirmed with histological staining for hypoxia-inducible factor 
      (HIF)-1alpha, a cellular transcription regulator which responds to local oxygen 
      levels. Using in vivo, longitudinal imaging methods we determined that the 
      placenta in the reduced uterine perfusion pressure rat model of preeclampsia is 
      hypoxic, and that this hypoxia is maintained through late gestation. Future work 
      will utilize these methods to assess the impact of novel therapeutics on 
      placental ischemia and the progression of preeclampsia.
FAU - Lawrence, Dylan J
AU  - Lawrence DJ
AD  - Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, 
      New Orleans, LA, 70118, USA.
FAU - Escott, Megan E
AU  - Escott ME
AD  - Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, 
      New Orleans, LA, 70118, USA.
FAU - Myers, Leann
AU  - Myers L
AD  - School of Public Health and Tropical Medicine, Tulane University, 1440 Canal St 
      #2400, New Orleans, LA, 70112, USA.
FAU - Intapad, Suttira
AU  - Intapad S
AD  - School of Medicine, Tulane University, 1430 Tulane Ave, New Orleans, LA, 70112, 
      USA.
FAU - Lindsey, Sarah H
AU  - Lindsey SH
AD  - School of Medicine, Tulane University, 1430 Tulane Ave, New Orleans, LA, 70112, 
      USA.
FAU - Bayer, Carolyn L
AU  - Bayer CL
AUID- ORCID: 0000-0003-0947-6892
AD  - Department of Biomedical Engineering, Tulane University, 500 Lindy Boggs Center, 
      New Orleans, LA, 70118, USA. carolynb@tulane.edu.
LA  - eng
GR  - R01 HL133619/HL/NHLBI NIH HHS/United States
GR  - U54 GM104940/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190124
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - *Cell Hypoxia
MH  - Disease Models, Animal
MH  - Female
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Ischemia/physiopathology
MH  - Longitudinal Studies
MH  - Oxygen/metabolism
MH  - Photoacoustic Techniques/*methods
MH  - Placenta/*physiopathology
MH  - *Placental Circulation
MH  - Pre-Eclampsia/*physiopathology
MH  - Pregnancy
MH  - Proteinuria
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Uterus/physiopathology
PMC - PMC6345947
COIS- The authors declare no competing interests.
EDAT- 2019/01/27 06:00
MHDA- 2020/08/04 06:00
PMCR- 2019/01/24
CRDT- 2019/01/26 06:00
PHST- 2018/06/22 00:00 [received]
PHST- 2018/12/05 00:00 [accepted]
PHST- 2019/01/26 06:00 [entrez]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2020/08/04 06:00 [medline]
PHST- 2019/01/24 00:00 [pmc-release]
AID - 10.1038/s41598-018-37310-2 [pii]
AID - 37310 [pii]
AID - 10.1038/s41598-018-37310-2 [doi]
PST - epublish
SO  - Sci Rep. 2019 Jan 24;9(1):558. doi: 10.1038/s41598-018-37310-2.