PMID- 30679991
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 17
IP  - 2
DP  - 2019 Feb
TI  - LncRNA UCA1 promotes cell proliferation, invasion and migration of laryngeal 
      squamous cell carcinoma cells by activating Wnt/beta-catenin signaling pathway.
PG  - 1182-1189
LID - 10.3892/etm.2018.7097 [doi]
AB  - In view of the poor prognosis of laryngeal squamous cell carcinoma (LSCC) and the 
      functionality of long non-coding (lnc)RNA UCA1 in different types of cancer, the 
      present study aimed to investigate the role of UCA1 in the development and 
      progression of LSCC. A total of 90 patients with LSCC and 90 healthy subjects 
      were enrolled in the present study. Expression levels of UCA1 in tumor tissues 
      and adjacent healthy tissues, as well as serum of patients with LSCC and normal 
      controls were detected by reverse transcription-quantitative polymerase chain 
      reaction. Receiver operating characteristic curve analysis was performed to 
      evaluate the diagnostic value of serum UCA1 for LSCC. Survival curves were 
      plotted using the Kaplan-Meier method and employed to evaluate the prognosic 
      values of serum UCA1 for LSCC. Cell proliferation, migration and invasion were 
      detected using the cell proliferation assay, and Transwell migration and invasion 
      assays, respectively. Expression levels of Wnt/beta-catenin-associated proteins were 
      detected by western blot analysis. Results indicated that the expression levels 
      of UCA1 were significantly higher in tumor tissues compared with adjacent healthy 
      tissues in the majority of patients with LSCC. In addition, serum levels of UCA1 
      were significantly higher in patients with LSCC coapred with healthy controls. 
      UCA1 overexpression promoted, whereas UCA1 knockdown inhibited the proliferation, 
      migration and invasion of LSCC cells. UCA1 overexpression activated the 
      Wnt/beta-catenin signaling pathway in LSCC cells, whereas treatment with Wnt 
      inhibitor reduced the enhancing effects of UCA1 overexpression on the 
      proliferation, migration and invasion of LSCC cells. The present findings suggest 
      that UCA1 can promote cell proliferation, invasion and migration of LSCC cells by 
      activating the Wnt/beta-catenin signaling pathway.
FAU - Sun, Suguang
AU  - Sun S
AD  - Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of 
      Wuhan, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430014, P.R. China.
FAU - Gong, Cheng
AU  - Gong C
AD  - Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of 
      Wuhan, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430014, P.R. China.
FAU - Yuan, Kun
AU  - Yuan K
AD  - Department of Otolaryngology-Head and Neck Surgery, The Central Hospital of 
      Wuhan, Tongji Medical College, Huazhong University of Science and Technology, 
      Wuhan, Hubei 430014, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20181213
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6327537
OTO - NOTNLM
OT  - UCA1
OT  - Wnt/beta-catenin signaling pathway
OT  - invasion
OT  - laryngeal squamous cell carcinoma
OT  - migration
OT  - proliferation
EDAT- 2019/01/27 06:00
MHDA- 2019/01/27 06:01
PMCR- 2018/12/13
CRDT- 2019/01/26 06:00
PHST- 2018/02/01 00:00 [received]
PHST- 2018/07/31 00:00 [accepted]
PHST- 2019/01/26 06:00 [entrez]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/01/27 06:01 [medline]
PHST- 2018/12/13 00:00 [pmc-release]
AID - ETM-0-0-7097 [pii]
AID - 10.3892/etm.2018.7097 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Feb;17(2):1182-1189. doi: 10.3892/etm.2018.7097. Epub 2018 Dec 
      13.