PMID- 30680244
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220331
IS  - 2080-4806 (Print)
IS  - 2080-4873 (Electronic)
IS  - 2080-4806 (Linking)
VI  - 71
IP  - 4
DP  - 2018
TI  - Impact of leukocytospermia on sperm dynamic motility parameters, DNA and 
      chromosomal integrity.
PG  - 470-475
LID - 10.5173/ceju.2018.1724 [doi]
AB  - INTRODUCTION: To characterize sperm dynamic motility patterns and chromatin 
      integrity in infertile men with leukocytospermia. MATERIAL AND METHODS: Fifty 
      patients with primary infertility and oligoasthenoteratozoospermia included in 
      this prospective, controlled, blind study. All patients underwent clinical 
      evaluation, semen peroxidase stain, computer aided semen analysis (CASA), sperm 
      DNA integrity evaluation with acridine orange test (AOT) and fluorescence in situ 
      hybridization (FISH) analysis of 18, X and Y chromosomes. Pregnancy outcomes were 
      documented following antibiotic treatment of patients with leukocytospermia. 
      RESULTS: Infertile men with leukocytospermia had significantly lower progressive 
      and total sperm motility percentages compared to the control group. Sperm dynamic 
      motility parameters by CASA including curvilinear, straight line and average 
      pathway velocities, straightness and amplitude of lateral head displacement were 
      significantly lower in leukocytospermia. Sperm DNA fragmentation index was 
      significantly higher in leukocytospermia. Percentages of sperm with disomy XY and 
      18 were significantly higher. These changes in sperm motility parameters and DNA 
      integrity correlated with the number of peroxidase positive leukocytes. Follow-up 
      of 23 of the 25 patients with leukocytospermia after antibiotic treatment 
      revealed significantly higher pregnancy rates in cured patients than in those 
      with persistent leukocytospermia. CONCLUSIONS: Leukocytospermia has a significant 
      impact on sperm dynamic motility patterns, DNA and chromosomal integrity in 
      infertile men which can adversely affect the likelihood of a successful 
      pregnancy.
FAU - Moubasher, Alaa
AU  - Moubasher A
AD  - Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut 
      University, Egypt.
FAU - Sayed, Heba
AU  - Sayed H
AD  - Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut 
      University, Egypt.
FAU - Mosaad, Eman
AU  - Mosaad E
AD  - Department of Clinical Pathology - South Egypt Cancer Institute, Assiut 
      University, Egypt.
FAU - Mahmoud, Ahmed
AU  - Mahmoud A
AD  - Department of Endocrinology and Andrology, Ghent University Hospital, Ghent, 
      Belgium.
FAU - Farag, Fawzy
AU  - Farag F
AD  - Department of Urology, Sohag University Hospital, Egypt.
FAU - Taha, Emad A
AU  - Taha EA
AD  - Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Assiut 
      University, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20180927
PL  - Poland
TA  - Cent European J Urol
JT  - Central European journal of urology
JID - 101587101
PMC - PMC6338814
OTO - NOTNLM
OT  - FISH
OT  - computer aided semen analysis
OT  - leukocytospermia
OT  - sperm DNA
EDAT- 2019/01/27 06:00
MHDA- 2019/01/27 06:01
PMCR- 2018/01/01
CRDT- 2019/01/26 06:00
PHST- 2018/05/27 00:00 [received]
PHST- 2018/08/17 00:00 [revised]
PHST- 2018/09/02 00:00 [accepted]
PHST- 2019/01/26 06:00 [entrez]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/01/27 06:01 [medline]
PHST- 2018/01/01 00:00 [pmc-release]
AID - 1724 [pii]
AID - 10.5173/ceju.2018.1724 [doi]
PST - ppublish
SO  - Cent European J Urol. 2018;71(4):470-475. doi: 10.5173/ceju.2018.1724. Epub 2018 
      Sep 27.