PMID- 30681775
OWN - NLM
STAT- MEDLINE
DCOM- 20200511
LR  - 20200511
IS  - 1749-4486 (Electronic)
IS  - 1749-4478 (Linking)
VI  - 44
IP  - 3
DP  - 2019 May
TI  - Association study of cell cycle proteins and human papillomavirus in laryngeal 
      cancer in Chinese population.
PG  - 323-329
LID - 10.1111/coa.13296 [doi]
AB  - OBJECTIVE: To investigate the role of human papillomavirus (HPV) in laryngeal 
      squamous cell carcinoma (LSCC) and analyse the relationship between HPV and the 
      expression of cell cycle-related proteins. DESIGN: The study consisted of LSCC 
      between 2005 and 2011 in Tongren Hospital. Clinical data such as age, sex, 
      smoking/alcohol consumption, and TNM stage were collected. HPV DNA and cell 
      cycle-related proteins were assessed in terms of clinical features. SETTING: 
      Single-centre study. PARTICIPANTS: A total of 332 LSCC patients were included in 
      the study. MAIN OUTCOME MEASURES: The presence of genotype-specific HPV DNA was 
      evaluated using PCR-RDB in formalin-fixed paraffin-embedded tissues. All samples 
      were also evaluated for p16(INK4A) , p21(WAF1/CIP1) , P53, Cyclin D1, and Ki67 
      immunohistochemical staining by tissue microarray. RESULTS: HPV DNA was detected 
      in 45 of 332 (13.55%) patients with LSCC, with HPV-16 being the predominant 
      genotype. The presence of HPV-16 DNA was significantly associated with basaloid 
      squamous cell carcinoma and cystic lymph node metastasis (P < 0.05). Of the 332 
      patients, 36 (10.84%) were scored as p16(INK4A) positivity and they were more 
      likely to be female (P < 0.05). Cyclin D1-positivity and p21(WAF1/CIP1) 
      -positivity were observed in 60.24% (200/332) and 40.66% (135/332), respectively. 
      In 114 cases (34.33%), LSCCs had moderate- to -strong p53 accumulation, which was 
      correlated with TNM stage (P < 0.05). HPV-16 DNA was correlated with p16(INK4A) 
      and manifested a higher Ki-67 labelling index and p21(WAF1/CIP1) expression than 
      HPV-16-negative tumours (P < 0.05). No relationship was observed between Cyclin 
      D1or P53 expression and HPV-16 infection (P > 0.05). CONCLUSION: HPV DNA was 
      detected in 13.55% patients with LSCC, with HPV-16 being the predominant genotype 
      and it was correlated with p16(INK4A) and manifested a higher Ki-67 labelling 
      index and p21(WAF1/CIP1) expression than HPV-16-negative tumours.
CI  - (c) 2019 John Wiley & Sons Ltd.
FAU - Cui, Lifang
AU  - Cui L
AUID- ORCID: 0000-0002-1178-204X
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
FAU - Qu, Congling
AU  - Qu C
AD  - The Department of Pathology, Beijing Shijitan Hospital, Capital Medical 
      University, Beijing, China.
FAU - Liu, Honggang
AU  - Liu H
AD  - Department of Pathology, Beijing Key Laboratory of Head and Neck Molecular 
      Diagnostic Pathology, Beijing Tongren Hospital, Capital Medical University, 
      Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20190227
PL  - England
TA  - Clin Otolaryngol
JT  - Clinical otolaryngology : official journal of ENT-UK ; official journal of 
      Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery
JID - 101247023
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/metabolism
MH  - Biopsy
MH  - Carcinoma, Squamous Cell/epidemiology/*pathology/virology
MH  - Cell Cycle Proteins/*metabolism
MH  - China/epidemiology
MH  - DNA, Viral/*analysis
MH  - Female
MH  - Follow-Up Studies
MH  - Human papillomavirus 16/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - Incidence
MH  - Laryngeal Neoplasms/epidemiology/*pathology/virology
MH  - Male
MH  - Middle Aged
MH  - Papillomavirus Infections/epidemiology/*pathology/virology
MH  - Retrospective Studies
OTO - NOTNLM
OT  - HPV
OT  - cell cycle
OT  - laryngeal squamous cell carcinoma
OT  - p16INK4A
EDAT- 2019/01/27 06:00
MHDA- 2020/05/12 06:00
CRDT- 2019/01/26 06:00
PHST- 2018/05/17 00:00 [received]
PHST- 2019/01/19 00:00 [accepted]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2020/05/12 06:00 [medline]
PHST- 2019/01/26 06:00 [entrez]
AID - 10.1111/coa.13296 [doi]
PST - ppublish
SO  - Clin Otolaryngol. 2019 May;44(3):323-329. doi: 10.1111/coa.13296. Epub 2019 Feb 
      27.