PMID- 30682548
OWN - NLM
STAT- MEDLINE
DCOM- 20200115
LR  - 20200115
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 69
DP  - 2019 Apr
TI  - Sequestration inside the yeast vacuole may enhance Helicobacter pylori survival 
      against stressful condition.
PG  - 127-133
LID - S1567-1348(18)30748-2 [pii]
LID - 10.1016/j.meegid.2019.01.029 [doi]
AB  - Vacuole of eukaryotic cells, beyond intracellular digestion plays additional 
      roles such as storage of nutrients that provide favorable conditions for 
      bacterial survival. In this study, occurrence of H. pylori inside the vacuole of 
      Candida yeast was studied and the role of vacuolating cytotoxin A (VacA) in 
      constructing the vacuole was discussed. One gastric Candida yeast was used for 
      Live/Dead stain and fluorescence in situ hybridization (FISH) with universal 
      bacterial probe. Yeast total DNA was used for amplification of full-length 
      bacterial 16S rDNA as well as H. pylori-specific 16S rDNA and vacA alleles. 
      Vacuoles were isolated from yeast cells and stained with fluorescent yeast 
      vacuole membrane marker MDY-64. DNA extracted from vacuoles was used for 
      amplification of H. pylori-specific 16S rDNA. Fluorescent microscopy showed 
      occurrence of viable bacteria inside the vacuole of intact Candida yeast cells. 
      FISH showed intracellular bacteria as fluorescent spots inside the vacuole of 
      mother and daughter yeast cells, suggesting bacterial transmission to next 
      generations of yeast. Sequencing of amplified products of bacterial 16S rDNA and 
      amplification of H. pylori 16S rDNA and vacA confirmed the identity of 
      intracellular bacteria as H. pylori. Isolated vacuoles were stained with 
      membrane-specific marker and H. pylori 16S rDNA was amplified from their DNA 
      content. Results of this study suggest yeast vacuole as a specialized niche for 
      H. pylori. It appears that sequestration inside the vacuole may enhance bacterial 
      survival.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Siavoshi, Farideh
AU  - Siavoshi F
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      Tehran University, Tehran, Iran. Electronic address: siavoshi@khayam.ut.ac.ir.
FAU - Heydari, Samira
AU  - Heydari S
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      Tehran University, Tehran, Iran.
FAU - Shafiee, Mahsa
AU  - Shafiee M
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      Tehran University, Tehran, Iran.
FAU - Ahmadi, Somayeh
AU  - Ahmadi S
AD  - Department of Microbiology, School of Biology, University College of Sciences, 
      Tehran University, Tehran, Iran.
FAU - Saniee, Parastoo
AU  - Saniee P
AD  - Department of Microbiology and Microbial Biotechnology, Faculty of Life Science 
      and Biotechnology, Shahid Beheshti University G.C, Tehran, Iran.
FAU - Sarrafnejad, Abdolfattah
AU  - Sarrafnejad A
AD  - Department of Immunology, School of Public Health, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Kolahdoozan, Shadi
AU  - Kolahdoozan S
AD  - Digestive Disease Research Institute, Tehran University of Medical Sciences, 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20190123
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - DNA, Bacterial
MH  - DNA, Ribosomal/genetics
MH  - Helicobacter pylori/*physiology
MH  - Humans
MH  - *Microbial Viability
MH  - Microscopy, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Stress, Physiological
MH  - Vacuoles/*microbiology
MH  - Yeasts/*metabolism
OTO - NOTNLM
OT  - Helicobacter pylori
OT  - Intracellular bacteria
OT  - Sequestration
OT  - Vacuole
OT  - Yeast
EDAT- 2019/01/27 06:00
MHDA- 2020/01/16 06:00
CRDT- 2019/01/26 06:00
PHST- 2018/09/27 00:00 [received]
PHST- 2018/12/24 00:00 [revised]
PHST- 2019/01/22 00:00 [accepted]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2020/01/16 06:00 [medline]
PHST- 2019/01/26 06:00 [entrez]
AID - S1567-1348(18)30748-2 [pii]
AID - 10.1016/j.meegid.2019.01.029 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2019 Apr;69:127-133. doi: 10.1016/j.meegid.2019.01.029. Epub 
      2019 Jan 23.