PMID- 30682715 OWN - NLM STAT- MEDLINE DCOM- 20190812 LR - 20190812 IS - 1879-1506 (Electronic) IS - 0003-9969 (Linking) VI - 99 DP - 2019 Mar TI - Oxytocin facilitates the proliferation, migration and osteogenic differentiation of human periodontal stem cells in vitro. PG - 126-133 LID - S0003-9969(18)30613-7 [pii] LID - 10.1016/j.archoralbio.2019.01.007 [doi] AB - OBJECTIVE: To explore the effect of oxytocin (OT) on the proliferation, migration, and osteogenic differentiation of periodontal ligament stem cells (PDLSCs) in vitro. DESIGN: PDLSCs were obtained by limiting dilution method. Immunofluorescence (IF), cell-counting kit-8 (CCK8), cell migration assay, Alizarin Red S staining, cetylpyridinium chloride (CPC) colorimetry, quantitative real-time polymerase chain reaction (qRT-PCR), and western blot analysis were used to examine the effect of OT on oxytocin receptor (OTR) expression, cell proliferation, migration and osteogenic differentiation of PDLSCs. RESULTS: Our study showed that PDLSCs expressed OTR. One hundred nanomolar OT exhibited the maximal effect on migration, while only 50 nM OT significantly promoted proliferation of PDLSCs, as well as mineralized nodule formation and calcium deposition (p < 0.05). Furthermore, 50 nM OT significantly up-regulated expression of osteogenesis-related genes-alkaline phosphatase (ALP), Collagen I (Col I), runt related transcription factor 2 (Runx 2), osteopontin (OPN) and osteocalcin (OCN)-at specific time points compared with osteogenic inductive medium (p < 0.05). In addition, western blot analysis demonstrated that 50 nM OT enhanced protein levels of ALP, Col I, and Runx 2 at day 7 and day 14 (p < 0.01), as well as activating the phosphorylation of extracellular-signal-regulated kinase (ERK) and protein kinase B (AKT) pathway; notably, 50 nM OT inhibited phosphorylation of the phosphatidylinositol 3-kinase (PI3K) pathway (p < 0.05). CONCLUSIONS: OT promoted proliferation, migration, and osteogenic differentiation of PDLSCs in vitro. Furthermore, the effect of OT on osteogenic differentiation was mediated through ERK and AKT pathway. Thus, OT may have potential for use in periodontal regeneration. CI - Copyright (c) 2019 Elsevier Ltd. All rights reserved. FAU - Ge, Bin AU - Ge B AD - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. FAU - Liu, Hongrui AU - Liu H AD - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. FAU - Liang, Qianyu AU - Liang Q AD - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. FAU - Shang, Lingling AU - Shang L AD - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. FAU - Wang, Ting AU - Wang T AD - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. FAU - Ge, Shaohua AU - Ge S AD - Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, Shandong Province, China; Department of Periodontology, School of Stomatology, Shandong University, Jinan, Shandong Province, China. Electronic address: shaohuage@sdu.edu.cn. LA - eng PT - Journal Article DEP - 20190119 PL - England TA - Arch Oral Biol JT - Archives of oral biology JID - 0116711 RN - 0 (Core Binding Factor Alpha 1 Subunit) RN - 0 (RUNX2 protein, human) RN - 0 (Receptors, Oxytocin) RN - 104982-03-8 (Osteocalcin) RN - 106441-73-0 (Osteopontin) RN - 50-56-6 (Oxytocin) RN - 9007-34-5 (Collagen) RN - EC 2.7.1.- (Phosphatidylinositol 3-Kinases) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases) RN - EC 3.1.3.1 (Alkaline Phosphatase) MH - Adolescent MH - Alkaline Phosphatase/genetics/metabolism MH - Cell Differentiation/*drug effects MH - Cell Movement/*drug effects MH - Cell Proliferation/*drug effects MH - Cells, Cultured MH - Child MH - Collagen/genetics/metabolism MH - Core Binding Factor Alpha 1 Subunit/genetics/metabolism MH - Extracellular Signal-Regulated MAP Kinases/metabolism MH - Gene Expression Regulation/drug effects MH - Humans MH - Osteocalcin/genetics/metabolism MH - Osteogenesis/*drug effects MH - Osteopontin/genetics/metabolism MH - Oxytocin/*pharmacology MH - Periodontal Ligament/cytology/*drug effects MH - Phosphatidylinositol 3-Kinases/metabolism MH - Proto-Oncogene Proteins c-akt/metabolism MH - Receptors, Oxytocin/metabolism MH - Signal Transduction MH - Stem Cells/cytology/*drug effects OTO - NOTNLM OT - Osteogenic differentiation OT - Oxytocin OT - Periodontal ligament stem cells EDAT- 2019/01/27 06:00 MHDA- 2019/08/14 06:00 CRDT- 2019/01/26 06:00 PHST- 2018/09/20 00:00 [received] PHST- 2018/12/12 00:00 [revised] PHST- 2019/01/15 00:00 [accepted] PHST- 2019/01/27 06:00 [pubmed] PHST- 2019/08/14 06:00 [medline] PHST- 2019/01/26 06:00 [entrez] AID - S0003-9969(18)30613-7 [pii] AID - 10.1016/j.archoralbio.2019.01.007 [doi] PST - ppublish SO - Arch Oral Biol. 2019 Mar;99:126-133. doi: 10.1016/j.archoralbio.2019.01.007. Epub 2019 Jan 19.