PMID- 30682870
OWN - NLM
STAT- MEDLINE
DCOM- 20191224
LR  - 20200309
IS  - 2072-6651 (Electronic)
IS  - 2072-6651 (Linking)
VI  - 11
IP  - 2
DP  - 2019 Jan 24
TI  - Transcriptomic Analysis of the Spider Venom Gland Reveals Venom Diversity and 
      Species Consanguinity.
LID - 10.3390/toxins11020068 [doi]
LID - 68
AB  - Selenocosmia jiafu (S. jiafu) has been recently identified as a new species of 
      spider in China. It lives in the same habitat as various other venomous spiders, 
      including Chilobrachys jingzhao (C. jingzhao), Selenocosmia huwena (S. huwena), 
      and Macrothele raveni (M. raveni). The venom from these different species of 
      spiders exhibits some similarities and some differences in terms of their 
      biochemical and electrophysiological properties. With the objective to illustrate 
      the diversity in venom peptide toxins and to establish the evolutionary 
      relationship between different spider species, we first performed transcriptomic 
      analysis on a cDNA library from the venom gland of S. jiafu. We identified 146 
      novel toxin-like sequences, which were classified into eighteen different 
      superfamilies. This transcriptome was then compared with that of C. jingzhao, 
      which revealed that the putative toxins from both spider venoms may have 
      originated from the same ancestor, although novel toxins evolved independently in 
      the two species. A BLAST search and pharmacological analysis revealed that the 
      two venoms have similar sodium channel modulation activity. This study provides 
      insights into the venom of two closely related species of spider, which will 
      prove useful towards understanding the structure and function of their toxins.
FAU - Hu, Zhaotun
AU  - Hu Z
AD  - Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of 
      Hunan Province, College of Biological and Food Engineering, Huaihua University, 
      Huaihua 418008, China. huzhaotun@hhtc.edu.cn.
AD  - The National and Local Joint Engineering Laboratory of Animal Peptide Drug 
      Development, College of Life Sciences, Hunan Normal University, Changsha 410081, 
      China. huzhaotun@hhtc.edu.cn.
FAU - Chen, Bo
AU  - Chen B
AD  - The National and Local Joint Engineering Laboratory of Animal Peptide Drug 
      Development, College of Life Sciences, Hunan Normal University, Changsha 410081, 
      China. hnnuchenboo@sina.com.
FAU - Xiao, Zhen
AU  - Xiao Z
AD  - The National and Local Joint Engineering Laboratory of Animal Peptide Drug 
      Development, College of Life Sciences, Hunan Normal University, Changsha 410081, 
      China. Zhen-xiao@outlook.com.
FAU - Zhou, Xi
AU  - Zhou X
AD  - The National and Local Joint Engineering Laboratory of Animal Peptide Drug 
      Development, College of Life Sciences, Hunan Normal University, Changsha 410081, 
      China. xizh@hunnu.edu.cn.
FAU - Liu, Zhonghua
AU  - Liu Z
AD  - The National and Local Joint Engineering Laboratory of Animal Peptide Drug 
      Development, College of Life Sciences, Hunan Normal University, Changsha 410081, 
      China. liuzh@hunnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190124
PL  - Switzerland
TA  - Toxins (Basel)
JT  - Toxins
JID - 101530765
RN  - 0 (Spider Venoms)
RN  - 0 (Voltage-Gated Sodium Channels)
SB  - IM
MH  - Exocrine Glands
MH  - Expressed Sequence Tags
MH  - Gene Expression Profiling
MH  - HEK293 Cells
MH  - Humans
MH  - Inbreeding
MH  - Phylogeny
MH  - Spider Venoms/*genetics/toxicity
MH  - Voltage-Gated Sodium Channels/physiology
PMC - PMC6409621
OTO - NOTNLM
OT  - Selenocosmia jiafu
OT  - cDNA library
OT  - diversity
OT  - spider
OT  - transcriptomic analysis
OT  - venom gland
COIS- The authors declare no conflict of interest.
EDAT- 2019/01/27 06:00
MHDA- 2019/12/25 06:00
PMCR- 2019/02/01
CRDT- 2019/01/27 06:00
PHST- 2018/12/25 00:00 [received]
PHST- 2019/01/09 00:00 [revised]
PHST- 2019/01/19 00:00 [accepted]
PHST- 2019/01/27 06:00 [entrez]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/12/25 06:00 [medline]
PHST- 2019/02/01 00:00 [pmc-release]
AID - toxins11020068 [pii]
AID - toxins-11-00068 [pii]
AID - 10.3390/toxins11020068 [doi]
PST - epublish
SO  - Toxins (Basel). 2019 Jan 24;11(2):68. doi: 10.3390/toxins11020068.