PMID- 30683673
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200225
IS  - 2046-6390 (Print)
IS  - 2046-6390 (Electronic)
IS  - 2046-6390 (Linking)
VI  - 8
IP  - 2
DP  - 2019 Feb 1
TI  - Tracking diphyodont development in miniature pigs in vitro and in vivo.
LID - 10.1242/bio.037036 [doi]
LID - bio037036
AB  - Abnormalities of tooth number in humans, such as agenesis and supernumerary tooth 
      formation, are closely related to diphyodont development. There is an increasing 
      demand to understand the molecular and cellular mechanisms behind diphyodont 
      development through the use of large animal models, since they are the most 
      similar to the mechanism of human tooth development. However, attempting to study 
      diphyodont development in large animals remains challenging due to large tooth 
      size, prolonged growth stage and embryo manipulation. Here, we characterized the 
      expression of possible genes for diphyodont development and odontogenesis of an 
      organoid bud from single cells of tooth germs in vitro using Wzhishan pig strain 
      (WZSP). Following this, we used a method of ectopic transplantation of tooth 
      germs at cap stage to dynamically track diphyodont development of tooth germs in 
      mouse subrenal capsules to overcome the restrictions in pig embryos. The results 
      showed that pig tooth germ at cap stage could restore diphyodont development and 
      maintain efficient long-term survival and growth in mouse subrenal capsules, 
      which is suitable for future manipulation of large mammalian tooth development. 
      Our pilot study provided an alternative for studying diphyodont development in 
      large mammals, which will further promote the use of pig as a diphyodont model 
      similar to humans for craniofacial development study.
CI  - (c) 2019. Published by The Company of Biologists Ltd.
FAU - Wang, Fu
AU  - Wang F
AUID- ORCID: 0000-0001-9615-1038
AD  - Molecular Laboratory for Gene Therapy & Tooth Regeneration, Beijing Key 
      Laboratory of Tooth Regeneration and Function Reconstruction, School of 
      Stomatology, Capital Medical University, Beijing 100050, China.
AD  - Department of Basic Oral Sciences, School of Stomatology, Dalian Medical 
      University, Dalian 116044, China.
FAU - Li, Guoqing
AU  - Li G
AUID- ORCID: 0000-0002-3463-6715
AD  - Molecular Laboratory for Gene Therapy & Tooth Regeneration, Beijing Key 
      Laboratory of Tooth Regeneration and Function Reconstruction, School of 
      Stomatology, Capital Medical University, Beijing 100050, China.
FAU - Wu, Zhifang
AU  - Wu Z
AUID- ORCID: 0000-0002-5370-4234
AD  - Molecular Laboratory for Gene Therapy & Tooth Regeneration, Beijing Key 
      Laboratory of Tooth Regeneration and Function Reconstruction, School of 
      Stomatology, Capital Medical University, Beijing 100050, China.
FAU - Fan, Zhipeng
AU  - Fan Z
AUID- ORCID: 0000-0003-0629-3476
AD  - Laboratory of Molecular Signaling and Stem Cells Therapy, Beijing Key Laboratory 
      of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital 
      Medical University, Beijing 100050, China.
FAU - Yang, Min
AU  - Yang M
AUID- ORCID: 0000-0002-6811-2709
AD  - Department of Basic Oral Sciences, School of Stomatology, Dalian Medical 
      University, Dalian 116044, China.
FAU - Wu, Tingting
AU  - Wu T
AUID- ORCID: 0000-0003-4375-6820
AD  - Molecular Laboratory for Gene Therapy & Tooth Regeneration, Beijing Key 
      Laboratory of Tooth Regeneration and Function Reconstruction, School of 
      Stomatology, Capital Medical University, Beijing 100050, China.
FAU - Wang, Jinsong
AU  - Wang J
AUID- ORCID: 0000-0002-7512-1582
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Capital Medical University, Beijing 100069, China.
FAU - Zhang, Chunmei
AU  - Zhang C
AUID- ORCID: 0000-0002-0122-348X
AD  - Molecular Laboratory for Gene Therapy & Tooth Regeneration, Beijing Key 
      Laboratory of Tooth Regeneration and Function Reconstruction, School of 
      Stomatology, Capital Medical University, Beijing 100050, China.
FAU - Wang, Songlin
AU  - Wang S
AUID- ORCID: 0000-0001-7572-3586
AD  - Molecular Laboratory for Gene Therapy & Tooth Regeneration, Beijing Key 
      Laboratory of Tooth Regeneration and Function Reconstruction, School of 
      Stomatology, Capital Medical University, Beijing 100050, China 
      slwang@ccmu.edu.cn.
AD  - Department of Biochemistry and Molecular Biology, School of Basic Medical 
      Sciences, Capital Medical University, Beijing 100069, China.
LA  - eng
PT  - Journal Article
DEP - 20190201
PL  - England
TA  - Biol Open
JT  - Biology open
JID - 101578018
PMC - PMC6398454
OTO - NOTNLM
OT  - Diphyodont development
OT  - Large animal
OT  - Successional tooth formation
OT  - Tooth replacement
OT  - Wuzhishan pig
COIS- Competing interestsThe authors declare no competing or financial interests.
EDAT- 2019/01/27 06:00
MHDA- 2019/01/27 06:01
PMCR- 2019/01/25
CRDT- 2019/01/27 06:00
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/01/27 06:01 [medline]
PHST- 2019/01/27 06:00 [entrez]
PHST- 2019/01/25 00:00 [pmc-release]
AID - bio.037036 [pii]
AID - BIO037036 [pii]
AID - 10.1242/bio.037036 [doi]
PST - epublish
SO  - Biol Open. 2019 Feb 1;8(2):bio037036. doi: 10.1242/bio.037036.