PMID- 30683757
OWN - NLM
STAT- MEDLINE
DCOM- 20190328
LR  - 20200301
IS  - 1943-2631 (Electronic)
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 211
IP  - 3
DP  - 2019 Mar
TI  - The Role of miRNAs in Drosophila melanogaster Male Courtship Behavior.
PG  - 925-942
LID - 10.1534/genetics.118.301901 [doi]
AB  - Drosophila melanogaster courtship, although stereotypical, continually changes 
      based on cues received from the courtship subject. Such adaptive responses are 
      mediated via rapid and widespread transcriptomic reprogramming, a characteristic 
      now widely attributed to microRNAs (miRNAs), along with other players. Here, we 
      conducted a large-scale miRNA knockout screen to identify miRNAs that affect 
      various parameters of male courtship behavior. Apart from identifying miRNAs that 
      impact male-female courtship, we observed that miR-957 mutants performed 
      significantly increased male-male courtship and "chaining" behavior, whereby 
      groups of males court one another. We tested the effect of miR-957 reduction in 
      specific neuronal cell clusters, identifying miR-957 activity in Doublesex 
      (DSX)-expressing and mushroom body clusters as an important regulator of 
      male-male courtship interactions. We further characterized the behavior of 
      miR-957 mutants and found that these males court male subjects vigorously, but do 
      not elicit courtship. Moreover, they fail to lower courtship efforts toward 
      females with higher levels of antiaphrodisiac pheromones. At the level of 
      individual pheromones, miR-957 males show a reduced inhibitory response to both 
      7-Tricosene (7-T) and cis-vaccenyl acetate, with the effect being more pronounced 
      in the case of 7-T. Overall, our results indicate that a single miRNA can 
      contribute to the regulation of complex behaviors, including detection or 
      processing of chemicals that control important survival strategies such as 
      chemical mate-guarding, and the maintenance of sex- and species-specific 
      courtship barriers.
CI  - Copyright (c) 2019 by the Genetics Society of America.
FAU - Iftikhar, Hina
AU  - Iftikhar H
AD  - Department of Biology, Texas A&M University, College Station, Texas 77843.
FAU - Johnson, Nicholas L
AU  - Johnson NL
AD  - Department of Biology, Texas A&M University, College Station, Texas 77843.
FAU - Marlatt, Matthew L
AU  - Marlatt ML
AD  - Department of Biology, Texas A&M University, College Station, Texas 77843.
FAU - Carney, Ginger E
AU  - Carney GE
AUID- ORCID: 0000-0002-3517-8728
AD  - Department of Biology, Texas A&M University, College Station, Texas 77843 
      gingercarney@uidaho.edu.
LA  - eng
SI  - figshare/10.25386/genetics.7522490
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190125
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (DSX protein, Drosophila)
RN  - 0 (Drosophila Proteins)
RN  - 0 (MIRN957 microRNA, Drosophila)
RN  - 0 (MicroRNAs)
RN  - 0 (Pheromones)
SB  - IM
MH  - Animals
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Drosophila Proteins/genetics/metabolism
MH  - Drosophila melanogaster
MH  - Female
MH  - Male
MH  - *Mating Preference, Animal
MH  - MicroRNAs/*genetics
MH  - Mushroom Bodies/metabolism
MH  - Mutation
MH  - Neurons/metabolism
MH  - Pheromones/metabolism
PMC - PMC6404249
OTO - NOTNLM
OT  - 7-T
OT  - Genetics of Sex
OT  - behavior
OT  - cVA
OT  - male courtship
OT  - miRNA
OT  - pheromones
EDAT- 2019/01/27 06:00
MHDA- 2019/03/29 06:00
PMCR- 2020/03/01
CRDT- 2019/01/27 06:00
PHST- 2018/12/26 00:00 [received]
PHST- 2019/01/19 00:00 [accepted]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/03/29 06:00 [medline]
PHST- 2019/01/27 06:00 [entrez]
PHST- 2020/03/01 00:00 [pmc-release]
AID - genetics.118.301901 [pii]
AID - 301901 [pii]
AID - 10.1534/genetics.118.301901 [doi]
PST - ppublish
SO  - Genetics. 2019 Mar;211(3):925-942. doi: 10.1534/genetics.118.301901. Epub 2019 
      Jan 25.