PMID- 30683830
OWN - NLM
STAT- MEDLINE
DCOM- 20190218
LR  - 20190219
IS  - 1899-1505 (Electronic)
IS  - 0867-5910 (Linking)
VI  - 69
IP  - 5
DP  - 2018 Oct
TI  - Characterization and biological effects of Hypericum extracts on 
      experimentally-induced - anxiety, oxidative stress and inflammation in rats.
LID - 10.26402/jpp.2018.5.13 [doi]
AB  - Anxiety disorders can associate with oxidative stress and immune system 
      alterations. Our study aimed to chemically analyze Hypericum maculatum (HM) and 
      Hypericum perforatum (HP) dry extracts and to evaluate their effects along with 
      quercetin (Q), on brain oxidative stress biomarkers: malondialdehyde (MDA), 
      catalase (CAT) and superoxide dismutase (SOD), inflammatory cytokines: 
      interleukin-1alpha, (IL-1alpha), IL-1beta, regulated on activation normal T cell expressed 
      and secreted (RANTES), interferon (IFN), monocyte chemoattractant protein-1 
      (MCP1), macrophage inflammatory protein (MIP) and serum corticosterone levels. 
      Nuclear transcription factor kappaB (NFkappaB) signaling pathway in the hippocampus and 
      frontal lobe in rats with N-methyl-9H-pyrido[5,4-b]indole-3-carboxamide (FG-7142) 
      experimental-induced anxiety were also investigated. The chemical analyses of 
      total hypericins were performed by spectrophotometric analysis and hypericin, 
      hyperforin and polyphenols derivatives were quantified by chromatographic 
      methods. The animals were divided in 6 groups: carboxymethylcellulose 2% (CMC); 
      CMC + FG; alprazolam (APZ) + FG; Q + FG; HM + FG; HP + FG. APZ (0.08 mg/kg b.w.), 
      Q (30 mg/kg b.w.), HM and HP (350 mg/kg b.w.) were orally administered for 21 
      days. FG (7.5 mg/kg b.w.) was intraperitoneally (i.p.) injected in a single dose. 
      Q and hypericum extracts (HpE) exerted anti-inflammatory (decreased IL-1alpha, IL-1beta, 
      MCP1, IFN and MIP mainly in hippocampus) and antioxidant effects (decreased MDA 
      levels, increased CAT and SOD activity), enhanced NFkappaB and pNFkappaB expressions in 
      the brain and reduced serum corticosterone levels. Our findings suggest that HpE 
      may improve anxiety-like behavior, offer brain protection by modulation of 
      oxidative stress and inflammation, and can contribute to overall biological 
      activity of natural compounds-rich diet.
FAU - Sevastre-Berghian, A C
AU  - Sevastre-Berghian AC
AD  - Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 
      Cluj-Napoca, Romania.
FAU - Toma, V A
AU  - Toma VA
AD  - Department Molecular Biology and Biotechnology, Faculty of Biology and Geology, 
      Babes-Bolyai University, Cluj-Napoca, Romania.
AD  - Institute of Biological Research, Department of Biochemistry and Experimental 
      Biology, branch of NIRDBS Bucharest, Cluj-Napoca, Romania.
AD  - NIRD for Isotopic and Molecular Technologies, Department of Molecular and 
      Biomolecular Physics, Cluj-Napoca, Romania.
FAU - Sevastre, B
AU  - Sevastre B
AD  - Department of Pathophysiology, Faculty of Veterinary Medicine, University of 
      Agricultural Science and Veterinary Medicine, Cluj-Napoca, Romania.
FAU - Hanganu, D
AU  - Hanganu D
AD  - Department of Pharmacognosy, Faculty of Pharmacy, "Iuliu Hatieganu" University of 
      Medicine and Pharmacy, Cluj-Napoca, Romania.
FAU - Vlase, L
AU  - Vlase L
AD  - Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of 
      Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, 
      Romania.
FAU - Benedec, D
AU  - Benedec D
AD  - Department of Pharmacognosy, Faculty of Pharmacy, "Iuliu Hatieganu" University of 
      Medicine and Pharmacy, Cluj-Napoca, Romania.
FAU - Oniga, I
AU  - Oniga I
AD  - Department of Pharmacognosy, Faculty of Pharmacy, "Iuliu Hatieganu" University of 
      Medicine and Pharmacy, Cluj-Napoca, Romania.
FAU - Baldea, I
AU  - Baldea I
AD  - Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 
      Cluj-Napoca, Romania.
FAU - Olteanu, D
AU  - Olteanu D
AD  - Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 
      Cluj-Napoca, Romania.
FAU - Moldovan, R
AU  - Moldovan R
AD  - Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 
      Cluj-Napoca, Romania.
FAU - Decea, N
AU  - Decea N
AD  - Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 
      Cluj-Napoca, Romania.
FAU - Filip, G A
AU  - Filip GA
AD  - Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 
      Cluj-Napoca, Romania. adrianafilip33@yahoo.com; gabriela.filip@umfcluj.ro.
FAU - Clichici, S V
AU  - Clichici SV
AD  - Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 
      Cluj-Napoca, Romania.
LA  - eng
PT  - Journal Article
DEP - 20190121
PL  - Poland
TA  - J Physiol Pharmacol
JT  - Journal of physiology and pharmacology : an official journal of the Polish 
      Physiological Society
JID - 9114501
RN  - 0 (Anti-Anxiety Agents)
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Cytokines)
RN  - 0 (Plant Extracts)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Animals
MH  - Anti-Anxiety Agents/*therapeutic use
MH  - Anti-Inflammatory Agents/*therapeutic use
MH  - Antioxidants/*therapeutic use
MH  - Anxiety/*drug therapy/metabolism
MH  - Brain/drug effects/metabolism
MH  - Corticosterone/blood
MH  - Cytokines/metabolism
MH  - *Hypericum
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Plant Extracts/*therapeutic use
MH  - Rats, Wistar
EDAT- 2019/01/27 06:00
MHDA- 2019/02/20 06:00
CRDT- 2019/01/27 06:00
PHST- 2018/09/09 00:00 [received]
PHST- 2018/10/30 00:00 [accepted]
PHST- 2019/01/27 06:00 [entrez]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/02/20 06:00 [medline]
AID - 10.26402/jpp.2018.5.13 [doi]
PST - ppublish
SO  - J Physiol Pharmacol. 2018 Oct;69(5). doi: 10.26402/jpp.2018.5.13. Epub 2019 Jan 
      21.