PMID- 30684909
OWN - NLM
STAT- MEDLINE
DCOM- 20190515
LR  - 20190515
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 123
DP  - 2019 Apr
TI  - Protein and gene expression of relevant enzymes and nuclear receptor of hepatic 
      lipid metabolism in grazing dairy cattle during the transition period.
PG  - 223-231
LID - S0034-5288(18)31316-X [pii]
LID - 10.1016/j.rvsc.2019.01.020 [doi]
AB  - We aimed to study the protein and gene expression of some hepatic enzymes of 
      lipid metabolism along with plasma biomarkers in grazing dairy cattle during the 
      transition period. Blood and liver biopsies from a group of eight multiparous 
      cows were sampled at -28, -14, +4, +14, +28 and +56 days relative to parturition. 
      Peak concentrations of NEFA and beta-hydroxybutyric acid with high 
      triacylglycerol content in the liver were recorded on day 4 postpartum. 
      Consistent with blood biomarkers, the gene expression of carnitine 
      palmitoyltransferase 1A (CPT1A) and acyl-CoA oxidase 1 (ACOX1) increased, whereas 
      that of diacylglycerol O-acyltransferase 1 (DGAT1) decreased. Nevertheless, CPT1A 
      protein expression did not change during all the period evaluated and ACOX1 
      protein expression increased on day 56 postpartum. In addition, the protein 
      expression of peroxisome proliferator-activated receptor alpha (PPAR-alpha) 
      increased on day 28 postpartum. On the other hand, DGAT1 protein expression 
      decreased on day 14 postpartum. As expected, the expression of genes associated 
      with fatty acid oxidation increased on the first days postpartum but, notably, 
      protein expression was highest after transition. Since most infectious diseases 
      and metabolic disorders in dairy cattle occur particularly on the first days 
      postpartum, it is not so clear whether an increase in the oxidation capacity of 
      the liver at that time could help to prevent disease and improve dairy 
      production. The valuable results about protein expression of enzymes involved in 
      liver lipid metabolism could help to better characterize the metabolism of dairy 
      cattle during the transition period.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Angeli, E
AU  - Angeli E
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias del Litoral, 
      Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
FAU - Trionfini, V
AU  - Trionfini V
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina.
FAU - Gareis, N C
AU  - Gareis NC
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias del Litoral, 
      Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
FAU - Matiller, V
AU  - Matiller V
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias del Litoral, 
      Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
FAU - Huber, E
AU  - Huber E
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina.
FAU - Rey, F
AU  - Rey F
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias del Litoral, 
      Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
FAU - Salvetti, N R
AU  - Salvetti NR
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias del Litoral, 
      Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
FAU - Ortega, H H
AU  - Ortega HH
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina; Facultad de Ciencias Veterinarias del Litoral, 
      Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina.
FAU - Hein, G J
AU  - Hein GJ
AD  - Laboratorio de Biologia Celular y Molecular Aplicada, Instituto de Ciencias 
      Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral 
      (UNL)/Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), 
      Esperanza, Santa Fe, Argentina; Centro Universitario Galvez, Universidad Nacional 
      del Litoral (UNL), Galvez, Santa Fe, Argentina. Electronic address: 
      ghein@santafe-conicet.gov.ar.
LA  - eng
PT  - Journal Article
DEP - 20190121
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Lipids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Triglycerides)
RN  - TZP1275679 (3-Hydroxybutyric Acid)
SB  - IM
MH  - 3-Hydroxybutyric Acid/blood
MH  - Animal Feed
MH  - Animal Husbandry
MH  - Animals
MH  - Cattle/*physiology
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Lactation/*physiology
MH  - Lipid Metabolism/genetics/*physiology
MH  - Lipids
MH  - Liver/*metabolism
MH  - Postpartum Period/*metabolism
MH  - Pregnancy
MH  - RNA, Messenger/genetics
MH  - Receptors, Cytoplasmic and Nuclear/genetics
MH  - Triglycerides/blood
OTO - NOTNLM
OT  - Dairy cattle
OT  - Lipid metabolism
OT  - Liver
OT  - PPAR-alpha
OT  - Transition period
EDAT- 2019/01/27 06:00
MHDA- 2019/05/16 06:00
CRDT- 2019/01/27 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2019/01/15 00:00 [revised]
PHST- 2019/01/20 00:00 [accepted]
PHST- 2019/01/27 06:00 [pubmed]
PHST- 2019/05/16 06:00 [medline]
PHST- 2019/01/27 06:00 [entrez]
AID - S0034-5288(18)31316-X [pii]
AID - 10.1016/j.rvsc.2019.01.020 [doi]
PST - ppublish
SO  - Res Vet Sci. 2019 Apr;123:223-231. doi: 10.1016/j.rvsc.2019.01.020. Epub 2019 Jan 
      21.