PMID- 30686191
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 100
IP  - 7
DP  - 2016 Jul
TI  - Survey, Molecular Detection, and Characterization of Geminiviruses Associated 
      with Cassava Mosaic Disease in Zambia.
PG  - 1379-1387
LID - 10.1094/PDIS-10-15-1170-RE [doi]
AB  - A survey was conducted from April to May 2014 in 214 farmers' fields located 
      across six major cassava-producing provinces (Western, Northwestern, Northern, 
      Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic 
      disease (CMD) and the species diversity of associated cassava mosaic 
      geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was 
      greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD 
      severity varied slightly between provinces, ranging from 2.78 in Eastern Province 
      to 3.00 in Northwestern Province. Polymerase chain reaction discrimination of 226 
      survey samples, coupled with complete DNA-A genome sequence analysis, revealed 
      the presence of African cassava mosaic virus (ACMV), East African cassava mosaic 
      virus (EACMV), and East African cassava mosaic Malawi virus (EACMMV) as single or 
      mixed infections of different proportions. Single-virus infections were 
      predominant, occurring in 62.8% (ACMV), 5.8% (EACMMV), and 2.2% (EACMV) of 
      samples relative to mixed-virus infections, which occurred in 19.5% (ACMV + 
      EACMMV), 0.4% (ACMV + EACMV), and 0.9% (ACMV + EACMV + EACMMV) of samples. 
      Phylogenetic analysis revealed the segregation of virus isolates from Zambia into 
      clades specific to ACMV, EACMV, and EACMMV, further confirming the presence of 
      all three viruses in Zambia. The results point to a greater diversity of CMG 
      across major cassava-growing provinces of Zambia and implicate contaminated 
      cassava cuttings in disease spread.
FAU - Mulenga, Rabson M
AU  - Mulenga RM
AD  - Zambia Agriculture Research Institute, Mount Makulu Central Research Station, 
      Chilanga, Lusaka, Zambia.
FAU - Legg, James P
AU  - Legg JP
AD  - International Institute of Tropical Agriculture, Dar es Salaam, Tanzania.
FAU - Ndunguru, Joseph
AU  - Ndunguru J
AD  - Mikocheni Agriculture Research Institute, Dar es Salaam, Tanzania.
FAU - Miano, Douglas W
AU  - Miano DW
AD  - University of Nairobi, College of Agriculture and Veterinary Sciences, Department 
      of Plant Sciences and Crop Protection, Kangemi, Nairobi, Kenya.
FAU - Mutitu, Eunice W
AU  - Mutitu EW
AD  - University of Nairobi, College of Agriculture and Veterinary Sciences, Department 
      of Plant Sciences and Crop Protection, Kangemi, Nairobi, Kenya.
FAU - Chikoti, Patrick C
AU  - Chikoti PC
AD  - Zambia Agriculture Research Institute, Mount Makulu Central Research Station.
FAU - Alabi, O J
AU  - Alabi OJ
AD  - Department of Plant Pathology & Microbiology, Texas A&M AgriLife Research and 
      Extension Center, Weslaco 78596.
LA  - eng
PT  - Journal Article
DEP - 20160408
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
EDAT- 2016/07/01 00:00
MHDA- 2016/07/01 00:01
CRDT- 2019/01/29 06:00
PHST- 2019/01/29 06:00 [entrez]
PHST- 2016/07/01 00:00 [pubmed]
PHST- 2016/07/01 00:01 [medline]
AID - 10.1094/PDIS-10-15-1170-RE [doi]
PST - ppublish
SO  - Plant Dis. 2016 Jul;100(7):1379-1387. doi: 10.1094/PDIS-10-15-1170-RE. Epub 2016 
      Apr 8.