PMID- 30686926 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 1313-2989 (Print) IS - 1313-2970 (Electronic) IS - 1313-2970 (Linking) IP - 817 DP - 2019 TI - Morphological and cytogenetic characteristics of Neobisium (Blothrus) slovacum Gulicka, 1977 (Pseudoscorpiones, Neobisiidae), the northernmost troglobitic species of the subgenus Blothrus in Europe. PG - 113-130 LID - 10.3897/zookeys.817.27189 [doi] AB - A redescription is provided of the adult, tritonymph and deutonymph life stages of the troglobitic Neobisium (Blothrus) slovacum Gulicka, 1977, which is known from Slovakia and Hungary. Material examined included 35 previously deposited museum specimens and 15 newly collected specimens. In addition, the karyotype and distribution of 18S rDNA clusters are described, using fluorescence in situ hybridization (FISH). The male karyotype of N.slovacum comprises 69 chromosomes, with a predominance of biarmed chromosomes, and an X0 sex chromosome system. Two pairs of signals for 18S rDNA on biarmed chromosomes (submetacentric and metacentric) of different sizes were identified. The present study provides the first information about the distribution of these clusters in the arachnid order Pseudoscorpiones. The geographic distribution of the species is summarized and mapped. Neobisiumslovacum is endemic to the Slovak and Aggtelek Karst area in southern Slovakia and north-western Hungary, where it has been recorded from 16 caves. One of these, Hacavska cave (in Slovakia), is the northernmost locality known for any species of the subgenus Blothrus. FAU - Cervena, Martina AU - Cervena M AUID- ORCID: 0000-0003-2634-8702 AD - Department of Zoology, Faculty of Natural Sciences, Comenius University, Mlynska dolina, Ilkovicova 6, SK-842 15 Bratislava, Slovakia Comenius University Bratislava Slovakia. FAU - Stahlavsky, Frantisek AU - Stahlavsky F AD - Department of Zoology, Faculty of Science, Charles University in Prague, Vinicna 7, CZ-128 44, Prague 2, Czech Republic Charles University Prague Czech Republic. FAU - Papac, Vladimir AU - Papac V AD - Slovak Caves Administration, Zeleznicna 31, SK-979 01 Rimavska Sobota, Slovakia Slovak Caves Administration Rimavska Sobota Slovakia. FAU - Kovac, Lubomir AU - Kovac L AUID- ORCID: 0000-0001-8194-2128 AD - Department of Zoology, Institute of Biology and Ecology, Faculty of Science, P.J. Safarik University, Srobarova 2, SK-041 54 Kosice, Slovakia Safarik University Kosice Slovakia. FAU - Jana, Christophoryova AU - Jana C AD - Department of Zoology, Faculty of Natural Sciences, Comenius University, Mlynska dolina, Ilkovicova 6, SK-842 15 Bratislava, Slovakia Comenius University Bratislava Slovakia. LA - eng PT - Journal Article DEP - 20190115 PL - Bulgaria TA - Zookeys JT - ZooKeys JID - 101497933 PMC - PMC6342903 OTO - NOTNLM OT - 18S rDNA OT - Distribution OT - FISH OT - Slovak Karst OT - endemic OT - sex chromosome OT - troglobitic EDAT- 2019/01/29 06:00 MHDA- 2019/01/29 06:01 PMCR- 2019/01/15 CRDT- 2019/01/29 06:00 PHST- 2018/06/04 00:00 [received] PHST- 2018/12/03 00:00 [accepted] PHST- 2019/01/29 06:00 [entrez] PHST- 2019/01/29 06:00 [pubmed] PHST- 2019/01/29 06:01 [medline] PHST- 2019/01/15 00:00 [pmc-release] AID - 10.3897/zookeys.817.27189 [doi] PST - epublish SO - Zookeys. 2019 Jan 15;(817):113-130. doi: 10.3897/zookeys.817.27189. eCollection 2019.