PMID- 30687272
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 9
DP  - 2018
TI  - Key Players and Individualists of Cyclic-di-GMP Signaling in Burkholderia 
      cenocepacia.
PG  - 3286
LID - 10.3389/fmicb.2018.03286 [doi]
LID - 3286
AB  - Burkholderia cenocepacia H111 is an opportunistic pathogen associated with 
      chronic lung infections in cystic fibrosis patients. Biofilm formation, motility 
      and virulence of B. cenocepacia are regulated by the second messenger cyclic 
      di-guanosine monophosphate (c-di-GMP). In the present study, we analyzed the role 
      of all 25 putative c-di-GMP metabolizing proteins of B. cenocepacia H111 with 
      respect to motility, colony morphology, pellicle formation, biofilm formation, 
      and virulence. We found that RpfR is a key regulator of c-di-GMP signaling in B. 
      cenocepacia, affecting a broad spectrum of phenotypes under various environmental 
      conditions. In addition, we identified Bcal2449 as a regulator of B. cenocepacia 
      virulence in Galleria mellonella larvae. While Bcal2449 consists of protein 
      domains that may catalyze both c-di-GMP synthesis and degradation, only the 
      latter was essential for larvae killing, suggesting that a decreased c-di-GMP 
      level mediated by the Bcal2449 protein is required for virulence of B. 
      cenocepacia. Finally, our work suggests that some individual proteins play a role 
      in regulating exclusively motility (CdpA), biofilm formation (Bcam1160) or both 
      (Bcam2836).
FAU - Richter, Anja M
AU  - Richter AM
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Fazli, Mustafa
AU  - Fazli M
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
FAU - Schmid, Nadine
AU  - Schmid N
AD  - Department of Microbiology, University of Zurich, Zurich, Switzerland.
FAU - Shilling, Rebecca
AU  - Shilling R
AD  - Department of Microbiology, University of Zurich, Zurich, Switzerland.
FAU - Suppiger, Angela
AU  - Suppiger A
AD  - Department of Microbiology, University of Zurich, Zurich, Switzerland.
FAU - Givskov, Michael
AU  - Givskov M
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
AD  - Singapore Centre for Environmental Life Sciences Engineering, Nanyang 
      Technological University, Singapore, Singapore.
FAU - Eberl, Leo
AU  - Eberl L
AD  - Department of Microbiology, University of Zurich, Zurich, Switzerland.
FAU - Tolker-Nielsen, Tim
AU  - Tolker-Nielsen T
AD  - Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of 
      Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
DEP - 20190110
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6335245
OTO - NOTNLM
OT  - Bcal2449
OT  - Burkholderia cenocepacia
OT  - Cyclic-di-GMP
OT  - GGDEF EAL domain proteins
OT  - RpfR
OT  - biofilm formation
OT  - motility
EDAT- 2019/01/29 06:00
MHDA- 2019/01/29 06:01
PMCR- 2019/01/10
CRDT- 2019/01/29 06:00
PHST- 2018/09/14 00:00 [received]
PHST- 2018/12/17 00:00 [accepted]
PHST- 2019/01/29 06:00 [entrez]
PHST- 2019/01/29 06:00 [pubmed]
PHST- 2019/01/29 06:01 [medline]
PHST- 2019/01/10 00:00 [pmc-release]
AID - 10.3389/fmicb.2018.03286 [doi]
PST - epublish
SO  - Front Microbiol. 2019 Jan 10;9:3286. doi: 10.3389/fmicb.2018.03286. eCollection 
      2018.