PMID- 3068791
OWN - NLM
STAT- MEDLINE
DCOM- 19890407
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 28
IP  - 6
DP  - 1988 Dec
TI  - Bone marrow derived cells express human T-cell lymphotropic virus type I 
      (HTLV-I)-related antigens in patients with multiple sclerosis.
PG  - 801-6
AB  - Mononuclear cells in peripheral blood (PB) and cerebrospinal fluid (CSF) of seven 
      patients and lymph nodes of three patients with clinically definite multiple 
      sclerosis (MS) expressed antigens that reacted with monoclonal antibodies (MoAb) 
      specific for HTLV-I p19 and p24 gag proteins. The labelled cells were visualized 
      with immunoperoxidase staining and indirect immunofluorescence and identified at 
      the ultrastructural level with immunogold technique. The frequency of these cells 
      was low, ranging from 0.1% to less than 0.01% in blood. In CSF it was 
      approximately 10 times lower. Cells reacting with anti-p19 Ab were found in all 
      MS samples, whereas cells reacting with anti-p24 Ab were found in 3 out of 6 
      blood samples and in 3 out of 7 CSF samples. All lymph nodes (3/3) obtained from 
      MS patients contained cells that reacted with anti-HTLV-I. p19 and p24 Ab. Cells 
      reacting with the same AB were detected in blood of one out of 12 healthy 
      controls. Stained cells were irregular, distinctly larger than lymphocytes, and 
      had abundant cytoplasm, suggesting that they may be monocytes/macrophages. 
      Immunogold particles were located in vacuole-like structures in the cytoplasm. 
      The presence in MS patients of cells that react with HTLV-I Ab indicates that a 
      human retroviral genome is being expressed, and suggests that a virus may be 
      present. Our data support a role for a human retrovirus in multiple sclerosis.
FAU - Sandberg-Wollheim, M
AU  - Sandberg-Wollheim M
AD  - Department of Neurology, University Hospital, Lund, Sweden.
FAU - Alumets, J
AU  - Alumets J
FAU - Biorklund, A
AU  - Biorklund A
FAU - Gay, R
AU  - Gay R
FAU - Gay, S
AU  - Gay S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Gene Products, gag)
RN  - 0 (HTLV-I Antigens)
RN  - 0 (Retroviridae Proteins)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Bone Marrow/*immunology
MH  - Cytoplasm/analysis
MH  - Fluorescent Antibody Technique
MH  - Gene Products, gag
MH  - HTLV-I Antigens/*analysis
MH  - Humans
MH  - Leukocytes, Mononuclear/analysis/ultrastructure
MH  - Lymph Nodes/analysis/ultrastructure
MH  - Multiple Sclerosis/blood/cerebrospinal fluid/*pathology
MH  - Retroviridae Proteins/immunology
EDAT- 1988/12/01 00:00
MHDA- 1988/12/01 00:01
CRDT- 1988/12/01 00:00
PHST- 1988/12/01 00:00 [pubmed]
PHST- 1988/12/01 00:01 [medline]
PHST- 1988/12/01 00:00 [entrez]
AID - 10.1111/j.1365-3083.1988.tb01514.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1988 Dec;28(6):801-6. doi: 10.1111/j.1365-3083.1988.tb01514.x.